Hair follicle stem cells were obtained from the bulge of rat whisker pads hair follicles as previously described.38 Briefly, the hair follicles were mechanically dissected from rat whisker pads and the bulge region was excised and explanted onto collagen (1 mg/mL, Roche; #11179179001) coated 4‐well plates. The explanted bulges were fed with alpha‐modified minimum essential medium (α‐MEM, Bio Idea, # BI‐1010‐05) that was supplemented with 1% penicillin/streptomycin (Bio Idea, # BI‐1203), 1% L‐Glu (Bio Idea, # BI‐1202) and either 10% fetal bovine serum (FBS, Bio Idea, # BI‐1201) (experimental group 1, i), 20% FBS (experimental group 2, ii) or 10% FBS plus 10% CEE (experimental group 3, iii). The bulges were incubated in a cell culture incubator at 37°C, 5% CO2. Following stem cell migration, cells were subcultured at nearly 80% confluency, using 0.25% Trypsin/EDTA (Bio‐Idea, # BI‐1602). In this study, the images of explanted bulges of different experimental groups at different days of in vitro culture (DIV: 3, 5, 7 and 11) were captured with a ZOE Fluorescent Cell Imager (Bio‐Rad). To compare the number of migrated stem cells between groups, cells were counted following first subculture, using trypan blue staining.
Fetal bovine serum (fbs)
Manufactured by Bioidea
Sourced in United States
FBS (Fetal Bovine Serum) is a common cell culture supplement derived from the blood of bovine fetuses. It provides a diverse array of proteins, growth factors, hormones, and other essential nutrients required for the growth and maintenance of cell lines in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Bioidea (11 mentions)
Penicillin, by Bioidea (8 mentions)
Streptomycin, by Bioidea (7 mentions)
Penicillin streptomycin, by Bioidea (6 mentions)
Rpmi 1640, by Bioidea (5 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
Penicillin streptomycin solution, by Merck Group (3 mentions)
Trypsin, by Merck Group (3 mentions)
Trypsin edta, by Bioidea (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Trypsin, by Bioidea (2 mentions)
Glycerol, by Merck Group (2 mentions)
L glutamine, by Bioidea (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Pen strep, by Bioidea (2 mentions)
Dulbecco s modified eagle s medium dmem, by Bioidea (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Saponin from quillaja bark, by Merck Group (1 mentions)
H 800 tem, by Hitachi (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
44 protocols using fetal bovine serum (fbs)
1
Optimizing Rat Whisker Follicle Stem Cell Culture
2
Saponin-Derived Nanostructure Characterization
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Periodontal ligament fibroblasts (PDLs) were purchased from the Sivan (Shiraz, Iran) company. The OSCC, was purchased from (Shayan Pars Cell Bank, Shiraz, Iran). DMEM culture medium, trypsin, fetal bovine serum (FBS), phosphate buffer solution, and MTT solutions were all purchased from BIO-IDEA (Iran). The commercial saponin used in this article was “Saponin from Quillaja bark” purchased from Sigma Aldrich (Darmstadt, Germany, CAS number: 8047-15-2). The characterization of the nanostructures were investigated using different analytical techniques. The FTIR tensor II Bruker (Berlin, Germany), AlFu and its derivatives were registered in the present case. Mira 3 Tescan (Kohoutovice, Czech Republic) and Hitachi H-800 TEM (Tokyo, Japan) were used for field emission scanning electron microscopy (EDX and FESEM) to evaluate the morphology and average particle size, respectively. Surface SA-3100 was used in conjunction with a pore analyzer (Beckman Coulter, Brea, CA, USA) to determine the specific surface area of the adsorbent developed and the average pore size.
3
Cytotoxicity of Carbon-Based Nanomaterials on A549 Cells
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A549 cells were purchased from the cell bank of Pasteur Institute. Using Dulbecco’s Modified Eagle’s medium (DMEM) (BIO-IDEA, Iran) containing 10% fetal bovine serum (BIO-IDEA, Iran), 100 μg/mL penicillin, and 100μg/mL streptomycin, the cells were cultured in an incubator. Afterwards, the cell culture process was completed and the cells were added to a 96-well culture plate (1×104 cells/mL). During 24 hours, the cells were allowed to get adhered to the floor of the wells. The cells were exposed to ten different concentrations of CBNs (0.1, 1, 10, 50, 100, 200, 300, 500, 600, and 1000 μg/mL) for 24, 48, and 72 hours. In order to increase the accuracy and reduce the error, we have separately repeated the tests three times. Also, the cells containing DMEM without CBNs were selected as the control group (Samples size: 10 (concentration)×3(Control)×3(Time)×3(repeat)=270).
4
Cultivation of MCF-7 Breast Cancer Cells
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MCF-7 cell line of human breast cancer (acquired from the University of Isfahan Cell Bank) was cultivated in COM medium made of Dulbecco’s modified Eagle’s minimum media (DMEM; BioIdea), with 10% fetal bovine serum (BioIdea) and 1% penicillin–streptomycin solution (Sigma, USA)45 .
5
Pectin, POS, and Galacturonic Acid Effects on MCF-7 Cells
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Human breast cancer MCF-7 cells (from the University of Isfahan Cell Line Bank) were cultivated in Dulbecco’s modified Eagle’s minimum media (DMEM; BioIdea), with 10% fetal bovine serum (BioIdea) and 1% penicillin–streptomycin solution (Sigma, USA). The cells were harvested onto six-well plates at a density of 1 × 104 cells per well and incubated at 37˚C in 5% CO2 and 95% humidity for 24 h before the experiment49 . The cells were then washed with phosphate-buffered saline (PBS, pH 7), and treated with fresh media containing 20 mg/mL of pectin, POS, and mono-galacturonic acid for 24. MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method was used to analyze the cell viability according to the Mosmann method50 (link). To determine the viability percentages in POS treated cancer cells, the cells were harvested again in the fresh medium and treated with 6 and 20 mg/mL of POS. Henceforth, propidium iodide (PI, Sigma) was used for the detection of the immortalized cells. The cells were analyzed using Flow Cytometer (Becton Dickinson FACS Calibure) and the distribution of cells was determined using CellQuest software51 (link).
6
Sebacic Acid-Glycerol-PLA Scaffold Development
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In this study, Sebacic acid (99%, Merck, Germany), Glycerol (99%, Merck, Germany), Poly Lactic Acid (PLA) (Mw = 200 kDa, Sigma-Aldrich), chloroform (Sigma-Aldrich), Dimethylformamide (DMF) (Merck, Germany), dimethyl thiazole diphenyltetrazolium bromide (MTT, Sigma-Aldrich), sodium citrate (Vacuette, Switzerland), Lipopolysaccharide (LPS, Sigma-Aldrich), dimethyl sulfoxide (DMSO) (Merck, Germany), Dulbecco’s Modified Eagle Medium (DMEM-high and F12-DMEM), fetal bovine serum (FBS), streptomycin, penicillin (Bioidea, Iran), and phosphate buffered saline tablets (PBS, Sigma-Aldrich) were used.
7
Cytocompatibility of Scaffolds on MG-63 Cells
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MG-63 cells from the National Cell Bank of Iran at the Pasteur Institute (NCBI, C555) were used to study the cytocompatibility of scaffolds. The scaffolds were sterilized via 30 min soaking in ethanol and consequent 20 min UV exposure. The cells (104 cells/mL) were seeded on the scaffolds and cultivated for 1 and 7 days in a complete culture medium based on Dulbecco’s Modified Eagle Medium (DMEM, BIO-IDEA, Iran) supplemented with 1% (v/v) streptomycin/penicillin (BIO-IDEA) and 10% (v/v) fetal bovine serum (Bioidea).
8
Evaluating 5-FU and Ketorolac against HT-29 Cells
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The human colorectal adenocarcinoma cell line HT-29 was obtained from the Pasteur Institute Cell Bank (Iran, Tehran) and was maintained in accordance with the instructions provided by the American Culture Collection. Culture medium RPMI 1640, fetal bovine serum (FBS), and penicillin-streptomycin (pen-strep) were purchased from BIO-IDEA (Iran). Thiazolyl blue tetrazolium was provided from Life Biolab (Germany). Trypsin and DMSO were purchased from Merck (Germany). 5-FU and ketorolac were obtained from Korea United Pharm (South Korea) and Caspian Tamin (Iran), respectively.
9
Characterization of Virulent E. coli
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Human colorectal adenocarcinoma (Caco-2) cells (code NCBI-C10094, Iran) and the murine macrophage-like cell line J774A.1 were prepared by the National Cell Bank of Iran (code NCBI-C483, Iran). Caco-2 cells were used to determine the adhesion and invasion ability of the E. coli isolates. The murine macrophage-like cell line J774A.1 was utilized to investigate the survival and replication inside macrophage cells. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (Bio-idea, Tehran, Iran) and 1% antibiotics, then incubated at 37°C in a humidified environment with a humidity of 95% and a CO2 concentration of 5% (INC246; Memmert, Schwabach, Germany). Further studies were conducted using logarithmically active cells.
10
Mycoplasma-free Gastric Cancer Cell Culture
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The gastric cancer cell-line AGS (as case cell-line) and mouse fibroblasts normal cellline (as the control normal cell-line) which were kind gifts from department of microbiology of Golestan university of medical sciences, were cultured in DMEM and RPMI-1640 media (Bio-idea, IR Iran, lot numbers: BI-1004 and BI-1007) respectively supplemented with heat-inactivated fetal bovine serum (10%) (Bio-idea, IR Iran, lot number: BI-1201) 100 U/ml penicillin, 100 µg/ml streptomycin (Bio-idea, IR Iran, lot number: BI-1203) and incubated under humidified environment at 37°C and 5% CO 2 as previously described 39 . The mycoplasma-free conditions of the celllines were proven with a Myco-Probe Mycoplasma Detection Kit (R&D Systems, Minneapolis, MN).
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