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Mk 8353

Manufactured by Selleck Chemicals
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Sourced in China, United States

The MK-8353 is a high-precision laboratory balance designed for accurate measurement of small quantities of materials. It features a digital display and a compact, space-saving design suitable for use in various laboratory settings.

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Lab products found in correlation

Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (1 mentions) Nocodazole, by Merck Group (1 mentions) Bapta am, by Merck Group (1 mentions) Ly294002, by Bio-Techne (1 mentions) U0126, by Bio-Techne (1 mentions) Ruxolitinib, by Novartis (1 mentions) Del 22379, by Selleck Chemicals (1 mentions) Sch772984, by Selleck Chemicals (1 mentions) α amanitin, by Merck Group (1 mentions) Bouin s solution, by Applygen (1 mentions) Bevacizumab, by Genentech (1 mentions)

4 protocols using mk 8353

1

Protein Expression and Localization

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Primary antibodies used in this study are listed the reagent table. Most of the primary antibodies were used for immunofluorescence at the dilution of 1:50 to 1:100 except VEcad (1:400) and α-Tub (1:500). For Western blotting, the primary antibody dilution was 1:500 for all, except Cx37 (1:250). For immunofluorescence, the species-specific secondary antibodies used in this study were conjugated with Alexa Fluor 405, 488, 546, 594, and 647 (1:500; Thermo Fisher Scientific) or with horseradish peroxidase-conjugated secondary antibodies (1:2,500/5,000; Cell Signaling Technologies) for Western blotting.
Cells were treated with DAPT (5 μM for 12 h, Cat#D5942; Sigma-Aldrich), LY294002 (10 μM for 1 h, or 2.5 μM for 12 h; Cat# 1130; Tocris), U0126 (10 μM for 1 h, or 2.5 μM for 12 h; Cat# 9903; Cell Signaling Technology), MK8353 (2.5 μM for 12 h; Cat# SCH900353; SelleckChem), nocodazole (10 μM for 30 min; Cat#M1404; Sigma-Aldrich), and BAPTA-AM (10 μM for 2 h, Cat#A1076; Sigma-Aldrich).
Acharya B.R., Fang J.S., Jeffery E.D., Chavkin N.W., Genet G., Vasavada H., Nelson E.A., Sheynkman G.M., Humphries M.J, & Hirschi K.K. (2023). Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest. Life Science Alliance, 6(8), e202201685.
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2

Potent ERK1/2 and JAK Inhibitors

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The ERK1/2 inhibitor LTT462 and ruxolitinib were provided by Novartis via Material Transfer Agreement, MK-8353, SCH772984 and DEL-22379 purchased from Sellekchem. LTT462 was administered by gavage at 75 mg/kg qd, ruxolitinib at 60 mg/kg bid and MK-8353 at 30 or 40 mg/kg bid. Inhibitors for in vitro use were stored at −20 °C in DMSO.
Brkic S., Stivala S., Santopolo A., Szybinski J., Jungius S., Passweg J.R., Tsakiris D., Dirnhofer S., Hutter G., Leonards K., Lischer H.E., Dettmer M.S., Neel B.G., Levine R.L, & Meyer S.C. (2021). Dual targeting of JAK2 and ERK interferes with the myeloproliferative neoplasm clone and enhances therapeutic efficacy. Leukemia, 35(10), 2875-2884.
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3

Antibody Identification and Enzyme Inhibitor Sourcing

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The anti-actin, anti-Dicer, anti-Drosha, anti-ERK and anti-p-ERK antibodies were from CST (Boston, MA, USA). The anti-p27 antibodies were purchased from Qianxun Biological (Guangzhou, China). Anti-rabbit IgG and -mouse IgG antibodies were from Proteintech (Chicago, IL, USA). The ERK2 inhibitor MK-8353 was purchased from Selleck (Shanghai, China). The α-amanitin was purchased through Sigma–Aldrich (St. Louis, MO, USA).
Yan Y., Chen S., Liao L., Gao S., Pang Y., Zhang X., Zhang H, & Xie Q. (2022). ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6. Viruses, 14(4), 805.
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4

Experimental Metastasis and Tumor Growth Assays

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The Committee on the Use of Live Animals of Bengbu Medical College approved all animal experiments (LDKPZ2021-182). For the tumor metastasis assay, six-week-old female NOD/SCID mice (Hfkbio, China) were injected with 1 × 106 KYSE70 cells via the tail vein (n = 6 per group). The mice were sacrificed after 6 weeks. Metastastic nodules in lung were fixed in Bouin’s solution (Applygen, China), and the number of metastases was determined. The tumor samples were embedded in paraffin, cut into 5-μm sections, and stained with hematoxylin and eosin (H&E). To evaluate tumor growth, six-week-old male BALB/c mice purchased from Hfkbio were subcutaneously injected with 1 × 106 differently treated KYSE70 cells. To inhibit tumor growth, five-week-old female BALB/c mice were given a subcutaneous injection of 1 × 106 KYSE70 cells. After one week, mice were equally divided into two groups according to the mean tumor volume, which was injected intraperitoneally with PBS (vehicle control) or drug regimen in PBS containing MK-8353 (10 mg/kg, three times per week, Selleck) and bevacizumab (15 mg/kg, three times per week, Genentech, USA) in PBS. Tumor size (length × width2 × 0.5) was measured at the indicated time points, and the mice were sacrificed after the tumor volume reached 1.5 cm3. The weight and volume of the xenografts were measured and imaged.
Pan Y., Liu J., Gao Y., Guo Y., Wang C., Liang Z., Wu M., Qian Y., Li Y., Shen J., Lu C, & Ma S. (2023). FBXW7 loss of function promotes esophageal squamous cell carcinoma progression via elevating MAP4 and ERK phosphorylation. Journal of Experimental & Clinical Cancer Research : CR, 42, 75.
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