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Chromatographic grade methanol and acetonitrile

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Chromatographic grade methanol and acetonitrile are high-purity solvents used in various analytical techniques, particularly in liquid chromatography. These solvents are formulated to meet the stringent requirements of chromatographic applications, ensuring consistent performance and reliable results.

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Lab products found in correlation

Acrylamide, by Thermo Fisher Scientific (1 mentions) Notoginsenoside r1, by Yuanye Bio-Technology (1 mentions) Milli q academic water purification system, by Merck Group (1 mentions)

Close spelling variants of this product

Chromatographic grade acetonitrile Chromatographic grade methanol Acetonitrile and methanol Chromatographic acetonitrile Methanol acetonitrile Acetonitrile Methanol

3 protocols using chromatographic grade methanol and acetonitrile

1

Quantitative Analysis of 4-MEI in Soy Sauce

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Some chemical reagents were purchased from Reagent Company: 4-MEI from Alfa Aesar (Tianjin, China); Acrylamide (AM), styrene (St), ethylene glycol dimethAcrylamide (EGDMA), and 2,2′-azobisissobutyronitrile (AIBN) from Alfa Aesar (Tianjin, China); both Ferric chloride (FeCl3·6H2O) and ferrous chloride (FeCl2·4H2O) from Fuchen Chemical Reagents Factory (Tianjin, China); Chromatographic grade methanol and acetonitrile from Merck Co. (Darmstadt, Germany); Dimethyl sulfoxide (DMSO), polyethylene glycol (PEG-6000), acetic acid, ammonium hydroxide and the other chemicals from Tianjin Chemical Reagent Co. (Tianjin, China). Ultrapure water was prepared by an ultra purification water system. Two soy sauce samples were obtained from a supermarket in Xi’an, named Shengchou (light color; Batch No: 4710; Zhengzhou Jiajia Flavor Industry Co., Ltd., Zhengzhou, China) and Laochou (dark color; Batch No: 20130820H; Jiangsu Hengshun Vinegar Industry Co., Ltd., Zhenjiang, China). All HPLC solutions were filtered through a 0.45 μm filter before use.
Feng Z., Lu Y., Zhao Y, & Ye H. (2017). Fast Extraction and Detection of 4-Methylimidazole in Soy Sauce Using Magnetic Molecularly Imprinted Polymer by HPLC. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(11), 1885.
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2

Osteoporosis Therapy Evaluation Protocol

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Chemicals and reagents used in this study included chromatographic-grade methanol and acetonitrile (Merck, Darmstadt, Germany); standard chemicals of catalpol, echinacoside, and acteoside (HPLC ≥98%, Shanghai Yuan-ye Bio-Technology Co., Ltd., Shanghai, China); and DEX (Dalian Meilun Biotech Co., Ltd., Dalian, China); ALN (MSD Pharmaceutical Co., Ltd., Hangzhou, China); Ca, P, ALP, and TRAP assay kits (Nanjing Jian Cheng Bioengineering Institute, Nanjing, China); enzyme-linked immunosorbent assay (ELISA) kits for BGP, DPD, and CTX-I (Xin Yu Biological Engineering Co., Ltd., Shanghai, China); antibodies specific for OPN, CYP17A1, CYP19A1, HSD11B1, and HSD3B2 (Abcom, Cambridge, UK); antibodies specific for RUNX2, anti-rabbit IgG, anti-mouse IgG, and GAPDH (CST, Danvers, MA, USA).
Xia T., Dong X., Jiang Y., Lin L., Dong Z., Shen Y., Xin H., Zhang Q, & Qin L. (2019). Metabolomics Profiling Reveals Rehmanniae Radix Preparata Extract Protects against Glucocorticoid-Induced Osteoporosis Mainly via Intervening Steroid Hormone Biosynthesis. Molecules, 24(2), 253.
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3

HPLC Analysis of Ginseng Constituents

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PG was collected from Jilin Province in China and purchased from Zhejiang Yingte Pharmaceutical Co., Ltd. (Hangzhou, China). PN was collected from Yunnan Province in China and purchased from KPC Pharmaceuticals, Inc. (Kunming, China). Both root plants were identified by Xingchu Gong, an associate professor at the College of Pharmaceutical Sciences at Zhejiang University, according to the Chinese Pharmacopoeia. Reference ginsenosides Rg1, Re, Rf, Rb1, Rc, Ro, Rb2, Rb3, Rd, and notoginsenoside R1 were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Analytical grade ethanol was purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. (Shanghai, China). Chromatographic-grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany) and that of phosphoric acid was purchased from the Tedia Company (Fairfield, IA, USA). Deionized water was obtained using a Milli-Q academic water purification system (Millipore, Milford, MA, USA).
Cheng J., Chen J., Liao J., Wang T., Shao X., Long J., Yang P., Li A., Wang Z., Lu X, & Fan X. (2023). High-throughput transcriptional profiling of perturbations by Panax ginseng saponins and Panax notoginseng saponins using TCM-seq. Journal of Pharmaceutical Analysis, 13(4), 376-387.
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