Anti phospho myosin light chain

Mouse monoclonal anti-Scribble (sc -55,543), anti-β-Actin (sc -69,879), anti-α E-catenin (sc-9988) (for immuno-staining) and anti-E-cadherin (sc-8426) (for immunoprecipitation) were from Santa Cruz Biotechnology. Antibodies specific for the N- and C-terminal region of Lgl1 were generated in rabbits [30 (link)]. Recombinant GFP antibodies were generated in rabbits [41 (link)]. Antibody specific for the C-terminal region of human NMII-A was generated in rabbits according to the method of [42 (link)]. Mouse monoclonal anti-E-cadherin (ab1416), anti-His (ab18184) and IgG (ab46540) were obtained from Abcam. Rabbit polyclonal anti-α E-catenin (C2081) was from Sigma. Rabbit polyclonal anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) (3674), anti-Lgl1 (D2B5A), anti-E-Cadherin (24E10) (for immuno-staining), anti-β-catenin (D10A8), and anti-Vimentin (D21H3) were obtained from Cell Signaling Technology. Anti-MBP (E8032) was from New England BioLAbs.
Horseradish peroxidase-conjugated goat anti-Rabbit secondary antibody, donkey anti-Goat conjugated to Alexa Fluor 488 and goat anti-Rabbit conjugated to Cy5 were obtained from Jackson ImmunoResearch Laboratories. Horseradish peroxidase-conjugated goat anti-Mouse (ab6789) and donkey anti-Mouse IgG H&L (Alexa Fluor 555) (ab150110) were obtained from Abcam. All antibodies were diluted 1:1000 for Western blot and 1:100 for immunofluorescence.

The following primary antibodies were used (dilution is indicated for immunofluorescence unless stated otherwise): anti-α-actinin (ab18061, Abcam, 1:100 dilution), anti-HA (3F10, Sigma-Aldrich, 1:200 dilution), anti-vinculin (#V9131, Sigma-Aldrich, 1:400 dilution), anti-zyxin (sc-6437, Santa Cruz, 1:40 dilution), anti-talin (#T3287, Sigma-Aldrich, 1:100 dilution), anti-β-actin (#MCA5775GA, Bio-Rad Laboratories, 1:200 dilution, 1:2000 for western blot), anti-γ-actin (#MCA5776GA, Bio-Rad Laboratories, 1:200 dilution, 1:2000 for western blot), anti-actin (#A2066, Sigma-Aldrich, 1:5000 dilution for western blot), anti-myosin IIA (#909802, BioLegend, 1:100 dilution), and anti-phospho-myosin light chain (#3671, Cell Signalling Technology, 1:100 dilution). Secondary antibodies conjugated to Alexa647, Alexa555, or Alexa568 were used (Life Technologies, 1:400 dilution). Actin was stained with Alexa488/Alexa633-conjugated phalloidin (Life Technologies, 1:200 dilution). Blebbistatin was purchased from Sigma-Aldrich (#B0560, 50 μM for 60 min).