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Eagle s minimum essential medium

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Eagle's minimum essential medium is a cell culture medium used for the growth and maintenance of various cell lines. It provides the essential nutrients and amino acids required for cell proliferation and survival.

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Lab products found in correlation

Fetal bovine serum (fbs), by Euroclone (2 mentions) Penicillin, by Euroclone (2 mentions) Streptomycin, by Euroclone (2 mentions) L glutamine, by Euroclone (2 mentions) Penicillin streptomycin, by Euroclone (1 mentions) Rpmi 1640 medium, by Euroclone (1 mentions) Dulbecco s modified eagle s medium, by Euroclone (1 mentions) Dulbecco s modified eagle medium, by Euroclone (1 mentions) Squalene, by Merck Group (1 mentions) Fetal calf serum (fcs), by Euroclone (1 mentions) Nonessential amino acid solution, by Euroclone (1 mentions) Trypsin edta, by Euroclone (1 mentions) Petri dishes, by Euroclone (1 mentions) Sodium pyruvate, by Euroclone (1 mentions)

4 protocols using eagle s minimum essential medium

1

Cell Line Authentication and Propagation

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Cell lines were tested and authenticated following the manufacturer’s instructions: DSMZ for NB4 and ATCC for all the others. HCT-116 and HT-29 (colon cancer), MCF7 (breast cancer), A549 (lung cancer), MiaPaCa (pancreas carcinoma), and A2058 (melanoma) cells were propagated in Dulbecco’s modified Eagle’s medium (Euroclone, Milan, Italy) with 10% fetal bovine serum (FBS) (Euroclone), 2 mM L-glutamine (Euroclone) and antibiotics (100 U/ml penicillin, 100 mg/ml streptomycin; Euroclone). NB4 (acute promyelocytic leukemia) and K562 (chronic myelogenous leukemia) cells were propagated in RPMI-1640 medium containing 4.5 g/L glucose (Euroclone) supplemented with 10% FBS (Euroclone), 100 U/mL penicillin–streptomycin (Euroclone) and 2 mM L-glutamine (Euroclone). MRC5 (normal human lung) cells were propagated in Eagle’s minimum essential medium (Euroclone) supplemented with 10% FBS (Euroclone), and 10 μg/ml gentamicin solution (Euroclone).
Sarno F., Papulino C., Franci G., Andersen J.H., Cautain B., Melardo C., Altucci L, & Nebbioso A. (2018). 3-Chloro-N′-(2-hydroxybenzylidene) benzohydrazide: An LSD1-Selective Inhibitor and Iron-Chelating Agent for Anticancer Therapy. Frontiers in Pharmacology, 9, 1006.
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2

Culturing Skin Fibroblasts from PD Patients

Cited 1 time
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Human skin biopsies from PD patients harboring the PINK1 or PARKIN mutations or healthy controls were obtained after signing an informed consent approved by the ethics committee of the IRCCS Foundation Neurological Institute ‘C. Besta’, Italy. Skin fibroblasts grown following the protocol described by us previously (Zanellati et al., 2015 (link)). Briefly, cells were grown in Ham’s F14 medium (Euroclone) supplemented with 10% FBS, 4% L-glutamine, 2% penicillin/streptomycin, 1.5% glucose, 1% insulin, 0.1% fibroblast growth factor, and 0.1% epidermal growth factor. Prior to the experiment, the growth medium was changed to Eagle’s minimum essential medium (Euroclone) supplemented with 10% FBS, 4% L-glutamine, and 2% penicillin/streptomycin. Human skin fibroblast cells used in our experiments were at passages 8–12. Phenotypic or genotypic data of the PD patients (with PINK1 and PARKIN mutations) and healthy controls are summarized in Table S6 and Key Resources Table.
Malty R.H., Aoki H., Kumar A., Phanse S., Amin S., Zhang Q., Minic Z., Goebels F., Musso G., Wu Z., Abou-tok H., Meyer M., Deineko V., Kassir S., Sidhu V., Jessulat M., Scott N.E., Xiong X., Vlasblom J., Prasad B., Foster L.J., Alberio T., Garavaglia B., Yu H., Bader G.D., Nakamura K., Parkinson J, & Babu M. (2017). A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals Molecular Determinants of Redox Homeostasis and NF-κB Signaling. Cell systems, 5(6), 564-577.e12.
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3

Immortalized Hepatic Stellate Cell Lines

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LX2 (a human immortalized hepatic stellate cell line), HSC-T6 (a rat immortalized hepatic stellate cell line), and HeLa (a human cervical carcinoma cell line) cells were cultured at 37°C in an atmosphere of 5% CO2 in Dulbecco's Modified Eagle Medium (Euroclone - Milan, Italy) supplemented with 10% Fetal Bovine Serum (Euroclone), 2 mM L-Glutamine and antibiotics. HepG2 cells (a human hepatocarcinoma cell line) were cultured at 37°C in an atmosphere of 5% CO2 in Eagle's Minimum Essential Medium (Euroclone) containing 10% Fetal Bovine Serum, 2 mM L-Glutamine and antibiotics.
D'Amore C., Orso G., Fusi F., Pagano M.A., Miotto G., Forgiarini A., De Martin S., Castellani G., Ribaudo G., Rennison D., Brimble M.A., Hopkins B., Ferrarese A, & Bova S. (2016). An NBD Derivative of the Selective Rat Toxicant Norbormide as a New Probe for Living Cell Imaging. Frontiers in Pharmacology, 7, 315.
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4

Investigating MK-7 and Simvastatin Effects

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Eagle’s minimum essential medium (MEM), trypsin-EDTA, penicillin, streptomycin, sodium pyruvate, L-glutamine, nonessential amino acid solution, fetal calf serum (FCS), plates, and Petri dishes were purchased from EuroClone. Squalene was purchased from Sigma-Aldrich (St Louis, MO, USA). RenaTris® capsules (500 mg) and MK-7 powder was supplied by PharmaNutra (Pisa, Italy), the contents of which were dissolved in 1.5 mL ethanol; the insoluble portion was separated by centrifugation. The supernatant was then evaporated under nitrogen flux and redissolved in 36 µL of ethanol in order to obtain a final concentration of 1.5 × 10−3M of MK-7. The final concentration of MK-7 was added to the cultured media was 1.8 µM. Simvastatin was dissolved in a physiological solution at 50 mM. Inorganic phosphate (Pi) solution was prepared by titrating 100 mM Na2HPO4 solution with 100 mM NaH2PO4 solution up to a pH of 7.4. All of the stock solutions were filtered through a 0.22 µM filter and stored at −20 °C. The Pi solution was stored at 4 °C.
Lupo M.G., Biancorosso N., Brilli E., Tarantino G., Adorni M.P., Vivian G., Salvalaio M., Dall’Acqua S., Sut S., Neutel C., Chen H., Bressan A., Faggin E., Rattazzi M, & Ferri N. (2020). Cholesterol-Lowering Action of a Novel Nutraceutical Combination in Uremic Rats: Insights into the Molecular Mechanism in a Hepatoma Cell Line. Nutrients, 12(2), 436.
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