Whole‐cell lysates were generated using Laemmli sample buffer (Bio‐Rad) and diluted in SDS‐PAGE sample buffer. Cell lysates were separated in 10% or 15% SDS‐PAGE and then transferred onto nitrocellulose membranes. The membranes were blocked with 5% milk and probed with the following antibodies: rabbit anti‐lamin‐B1 (1:5,000; Abcam), mouse anti‐lamin‐A/C (1:5,000; Active Motif), rabbit anti‐TNF‐α (1:1,000; Cell Signaling), rabbit anti‐IL‐1β (1:1,000; Cell Signaling), rabbit anti‐IL‐6 (1:1,000; Novus biological), rabbit anti‐IL‐1α (1:2000, Santa Cruz), and mouse anti‐β‐actin (1:4,000; Sigma, AC‐15). Antibodies were detected with HRP‐conjugated anti‐mouse (1:10,000) or anti‐rabbit (1:10,000) antibodies and West Pico Substrate (Thermo Scientific).
Mouse anti lamin a c
Manufactured by Active Motif
Mouse anti-lamin-A/C is a primary antibody that specifically binds to the lamin A and lamin C proteins, which are structural components of the cell nucleus. This antibody can be used to detect and localize these proteins in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti lamin b1, by Abcam (2 mentions)
Rabbit anti tnf α, by Cell Signaling Technology (1 mentions)
Rabbit anti il 1β, by Cell Signaling Technology (1 mentions)
West pico substrate, by Thermo Fisher Scientific (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Laemmli sample buffer, by Bio-Rad (1 mentions)
Streptavidin hrp, by LI COR (1 mentions)
Odyssey clx machine, by LI COR (1 mentions)
Odyssey fc machine, by LI COR (1 mentions)
Streptavidin hrp, by GE Healthcare (1 mentions)
Rabbit anti emerin, by Santa Cruz Biotechnology (1 mentions)
Mouse anti sc35, by Merck Group (1 mentions)
2 protocols using mouse anti lamin a c
1
Western Blot Analysis of Inflammatory Markers
2
Biotinylated Protein Identification via Immunoblotting
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The APEX2 reaction was performed as described above and lysed in RIPA. Aliquots were taken for input, post-streptavidin flow-through, and StrePD. Proteins were separated on an SDS-PAGE gel and transferred to nitrocellulose for Western blotting with the indicated reagents/antibodies. Detection reagents used were streptavidin-HRP (1:1,000; GE Healthcare; RPN1231V), rabbit anti-lamin-B1 (1:10,000; Abcam; ab16048), mouse anti-lamin-A/C (1:5,000; Active Motif; 39287), rabbit anti-emerin (1:5,000; Santa Cruz; sc-15378), mouse anti-SC-35 (1:2,500, Sigma-Aldrich; S4045), rabbit anti-HNRNPA1 (1:2,500; ProteinTech; 11176-1-AP), and rabbit anti-SRSF1 (1:2,500; ProteinTech; 12929-2-AP). Imaging for streptavidin-HRP was done on a Licor Odyssey Fc machine. All other Western blots were imaged with a Licor Odyssey CLx machine.
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