Dimethyl sulfoxide (dmso)

Manufactured by Santa Cruz Biotechnology

Sourced in United States, Germany

DMSO (Dimethyl Sulfoxide) is a colorless, odorless liquid that is widely used as a solvent in chemical, biological, and pharmaceutical applications. It has a high boiling point and is miscible with water and many organic solvents. DMSO is commonly used to dissolve and stabilize a variety of compounds, including proteins, peptides, and small molecules.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Tissue culture grade dimethyl sulfoxide (DMSO, (2 citations)

67 protocols using dimethyl sulfoxide (dmso)

Measuring Mitophagy in SH-SY5Y Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

mitoSRAI POE SH-SY5Y cells were seeded in a 96-well plate (PhenoPlate 96-well microplate, PerkinElmer, 6055302), pre-treated with MG149 or NU9056 for 3 h according to desired concentrations, and subsequently with DMSO (Santa Cruz, sc-202581) or O/A for 3 h.
Equal volumes of DMSO (Santa Cruz, sc-202581) were used as no treatment controls.
After fixation, nuclei were counterstained with DRAQ5 as described above, and then imaged on the Opera Phenix (PerkinElmer) with 63X water objective.
Images were then analysed using the Columbus 2.8 analysis system (PerkinElmer) to measure the SRAI mitophagy index, which is the ratio between the area of lysosomal mitochondria (corresponding to the total YPet negative TOLLES area) and the total mitochondrial area (corresponding to total TOLLES area) per well.

de Talhouët C., Esteras N., Soutar M.P., O’Callaghan B, & Plun-Favreau H. (2024). KAT8 compound inhibition inhibits the initial steps of PINK1-dependant mitophagy. Scientific Reports, 14, 11721.

+ Open protocol

+ Expand

Targeting KAT5 and KAT8 with MG149 and NU9056

Check if the same lab product or an alternative is used in the 5 most similar protocols

MG149 targets KAT5 and KAT8 with IC₅₀ values of 74 µM and 47 µM respectively^{24 (link)}. NU9056 targets KAT5 with an IC₅₀ value of 2 µM. Both small molecules were reconstituted in DMSO (Santa Cruz, sc-202581) before treatments.
For immunofluorescence, both compounds were diluted in DMEM (Gibco, 11995-065) so that 25 uL of each diluted compound was added per well of a 96-well plate (PhenoPlate 96-well microplate, PerkinElmer, 6055302) to make up desired concentrations.
For immunoblotting, concentrations of 10 µM for NU9056 and 100 µM for MG149 were added to corresponding wells.
Equal volumes of DMSO (Santa Cruz, sc-202581) were used as no treatment controls.

de Talhouët C., Esteras N., Soutar M.P., O’Callaghan B, & Plun-Favreau H. (2024). KAT8 compound inhibition inhibits the initial steps of PINK1-dependant mitophagy. Scientific Reports, 14, 11721.

+ Open protocol

+ Expand

Cardenolide Preparation for Drosophila Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cardenolides ouabain (≥ 95% purity), digoxin (≥ 95% purity), and digitoxin (≥ 92% purity) - listed in decreasing order of polarity (Frick and Wink, 1995 (link)) - were obtained from Sigma-Aldrich (St Louis, MO, USA). The cardenolides were dissolved in the solvent dimethyl sulfoxide (DMSO; ≥ 99% purity; Santa Cruz Biotechnology, Dallas, TX, USA), which was subsequently diluted to a final concentration of 0.1% DMSO with a 5% sucrose (weight/volume) fly food solution. The concentration of 0.1% DMSO was chosen to be below the experimentally determined “no observed adverse effect level” of 0.3% for Drosophila (Nazir et al., 2003 ). The cardenolide concentrations of the solutions were confirmed with high-performance liquid chromatography (HPLC) as described by Züst and Agrawal (2016) . Information on the LD50 of the three cardenolides was found in the NIH TOXNET ChemIDplus database (visited: 09/09/2016; oubain: http://chem.sis.nlm.nih.gov/chemidplus/rn/630-60-4; digoxin: http://chem.sis.nlm.nih.gov/chemidplus/rn/20830-75-5; digitoxin: http://chem.sis.nlm.nih.gov/chemidplus/rn/71-63-6).

Groen S.C., LaPlante E.R., Alexandre N.M., Agrawal A.A., Dobler S, & Whiteman N.K. (2016). Multidrug transporters and organic anion transporting polypeptides protect insects against the toxic effects of cardenolides. Insect biochemistry and molecular biology, 81, 51-61.

+ Open protocol

+ Expand

Circulating RNA and Apoptosis Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

circRNA_001937, miRNA-597-3p and FOSL2 mRNA primer sequences were purchased from Kangcheng Co., Ltd. Anti-FOSL2 primary antibody (1:500; cat. no. H00116173-B01P) and anti-GAPDH primary antibody (1:1,000; cat. no. R2655) were purchased from Sigma-Aldrich (Merck KGaA). RPMI-1640 medium, fetal bovine serum (FBS), crystal violet, Annexin V fluorescein isothiocyanate (FITC)/propidium iodide (PI) Cell Apoptosis Detection kit (cat. no. sc-4252 AK), MTT assays, Transwell plates, Matrigel and dimethyl sulfoxide were purchased from Santa Cruz Biotechnology, Inc.

Gao L., Jin H.J., Zhang D, & Lin Q. (2020). Silencing circRNA_001937 may inhibit cutaneous squamous cell carcinoma proliferation and induce apoptosis by preventing the sponging of the miRNA-597-3p/FOSL2 pathway. International Journal of Molecular Medicine, 46(5), 1653-1660.

+ Open protocol

+ Expand

MTT Assay for Organoid Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following treatment, organoids were washed in PBS twice and incubated in fresh media in the presence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (0.5 mg/mL, Millipore Sigma) for 1 h at 37 °C. The media was removed, and cells were lysed in 500 μL dimethyl sulfoxide (Santa Cruz, Dallas, TX, USA). Samples were measured at 595 nm in triplicate using a microplate reader (Spark 10M, Tecan, Männedorf, Switzerland). Measurements were normalized to the organoid weight.

Zhang I., Lépine P., Han C., Lacalle-Aurioles M., Chen C.X., Haag R., Durcan T.M, & Maysinger D. (2020). Nanotherapeutic Modulation of Human Neural Cells and Glioblastoma in Organoids and Monocultures. Cells, 9(11), 2434.

+ Open protocol

+ Expand

Rat Model of DDS-Induced Poisoning

Check if the same lab product or an alternative is used in the 5 most similar protocols

Forty-five drug-naïve male Sprague-Dawley rats weighing 250 to 300 g were used. The animals were housed in a controlled environment for 3 to 7 days and were allowed free access to food and water. All animals were fasted for 8 hours before the experiment, but had free access to water. The rats were anesthetized by inhalation of 3% isoflurane using an anesthetizing box for 60 to 90 seconds. The tail vein was cannulated using a 24-gauge catheter (BD Insyte Autoguard; Becton-Dickinson, Franklin Lake, NJ, USA) to administer the medication. DDS (40 mg/kg; Santa Cruz Biotechnology, Santa Cruz, CA, USA) in dimethyl sulfoxide (1 mg/kg, Santa Cruz Biotechnology) was administered to the rats via oral gavage to induce poisoning.

Kang C., Kim D.H., Kim T., Lee S.H., Jeong J.H., Lee S.B., Kim J.H., Jung M.H., Lee K.W, & Park I.S. (2018). Therapeutic effect of ascorbic acid on dapsone-induced methemoglobinemia in rats. Clinical and Experimental Emergency Medicine, 5(3), 192-198.

+ Open protocol

+ Expand

Gastric Cancer Cell Line Cultivation and Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human gastric epithelial cell line GES‐1, and human gastric cancer cell lines, such as SGC7901, MGC803 and HGC27 cells, were obtained from the Cell Culture Centre, Beijing Institute of Basic Medical Science of the Chinese Academy of Medical Science (Beijing, China). The cells were cultured in RPMI Medium 1640 (Gibco, Grand Island, NY, USA) containing 10% foetal bovine serum (FBS, Kangyuan Biology, China). JS‐K was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and dissolved in Dimethyl Sulfoxide (DMSO). Z‐VAD‐FMK (50 μmol/L), Z‐LEHD‐FMK (50 μmol/L) and Z‐DEVD‐FMK (50 μmol/L) were purchased from Medchem Express (Beijing, China). 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide (MTT), carboxy‐PTIO (100 μmol/L) and NAC (500 μmol/L) were purchased from Sigma‐Aldrich (St. Louis, MO, USA).

Zhao X., Cai A., Peng Z., Liang W., Xi H., Li P., Chen G., Yu J, & Chen L. (2019). JS‐K induces reactive oxygen species‐dependent anti‐cancer effects by targeting mitochondria respiratory chain complexes in gastric cancer. Journal of Cellular and Molecular Medicine, 23(4), 2489-2504.

+ Open protocol

+ Expand

Hypoxia Mimicry in Compound Treatments

Check if the same lab product or an alternative is used in the 5 most similar protocols

Telaprevir (TPV) and staurosporine (STS) were obtained from AdooQ Biosciences and asunaprevir (ASV) was obtained by custom synthesis (Acme Bioscience). ASV is also commercially available as a research reagent. AP20187 (AP) was purchased from ApexBio. Stocks of each compound were made at 1000× of the final concentration in dimethysulfoxide (DMSO, Santa Cruz Biotechnology) and stored at −20 °C. For experiments with transfected plasmids, ASV and TPV were applied to cells directly prior to transfection, or along with transfection reagents, unless otherwise indicated. Cobalt(II) chloride hexahydrate (CoCl2, Sigma Aldrich) was dissolved in water to make a 400-mM (500×) stock. In applicable experiments, cells were incubated in 800 μM CoCl2 for the final 7 h before harvest, in order to simulate hypoxia.

Jacobs C.L., Badiee R.K, & Lin M.Z. (2018). StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins. Nature methods, 15(7), 523-526.

+ Open protocol

+ Expand

Biochemical and behavioral analysis of neuropathic pain

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following reagents and instruments were used: 5% TNBS (Sigma, St Louis, MO, United States); phospho (p)MEK antibody (Cell Signaling Technology, Danvers, MA, United States); pERK antibody (Cell Signaling Technology); pCREB antibody (Cell Signaling Technology); MEK inhibitor U0126 (Calbiochem, Darmstadt, Germany); DMSO (Santa Cruz Biotechnology, Dallas, TX, United States); horseradish peroxidase goat anti-rabbit IgG (Beyotime, Shanghai, China); Von Frey filaments (Stoelting Co, Wood Dale, IL, United States); BME2410A thermal stimulator (Institute of Medical Biology, Chinese Academy of Medical Sciences, Beijing, China); electrophoresis system: Mini-PROTEAN 3 cell (Bio-Rad, Hercules, CA, United States); imaging system: ChemiDoc^TM (Bio-Rad, Hercules, CA, United States); PCR primers ABI 7300 (Thermo Fisher Scientific, Waltham, MA, United States).

Li Z.Y., Huang Y., Yang Y.T., Zhang D., Zhao Y., Hong J., Liu J., Wu L.J., Zhang C.H., Wu H.G., Zhang J, & Ma X.P. (2017). Moxibustion eases chronic inflammatory visceral pain through regulating MEK, ERK and CREB in rats. World Journal of Gastroenterology, 23(34), 6220-6230.

+ Open protocol

+ Expand

Osteoprotegerin Regulation of Angiogenesis and Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human osteoprotegerin (OPG) was purchased from BioSource International (Camarillo, CA). 1,25-dihydroxyvitamin D₃ (1,25D₃) was from BIOMOL International (Plymouth Meeting, PA). Dimethylsulfoxide (DMSO), PGE₂, and indomethacin were obtained from Sigma Chemical Co. (St. Louis, MO). NS-398, a selective COX-2 inhibitor, was purchased from Cayman Chemical Co. (Ann Arbor, MI). For in vitro use, indomethacin and NS-398 were reconstituted in DMSO and diluted with culture media. The final concentration of DMSO in the culture media was less than 0.1%.
Polyclonal rabbit anti-vascular endothelial growth factor-A (VEGF-A) and polyclonal rabbit anti-proliferation cell nuclear antigen (PCNA) were from Santa Cruz Biotechnology (Santa Cruz, CA). Polyclonal rabbit anti-COX-2 and polyclonal rabbit anti-factor VIII were from Zymed Laboratories, Inc. (South San Francisco, CA).

Tsutsumimoto T., Williams P, & Yoneda T. (2014). The SK-N-AS human neuroblastoma cell line develops osteolytic bone metastases with increased angiogenesis and COX-2 expression. Journal of Bone Oncology, 3(3-4), 67-76.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!