Log In Sign up
The largest database of trusted experimental protocols

Mitoxpess probe solution

Manufactured by Cayman Chemical
Visit Supplier

The MitoXpress probe solution is a fluorescent reagent used to measure oxygen levels in cell culture and biological samples. It functions as a sensitive, ratiometric probe that reports on the oxygen concentration in the environment.

Automatically generated - may contain errors

Lab products found in correlation

Spectramax m2e microplate reader, by Molecular Devices (2 mentions)

2 protocols using mitoxpess probe solution

1

Mitochondrial Respiration Assay in RCC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
RCC4 or RCC10 cells ectopically expressing vector or FBP1 were seeded into black opaque 96-well plate at a density of 70, 000 cells per well. After attachment, culture medium within each well was replaced with 150 µL oxygenated fresh medium, supplemented with 10 µL MitoXpess probe solution (Cayman Chemical). Antimycin A was used as negative control. 100 µL mineral oil was dispensed to overlay each well, and the whole plate was monitored using SpectraMax m2e microplate reader (Molecular Devices) at 37 °C. Wavelengths are set to 380 nm for excitation and 650 nm for emission.
Li B., Qiu B., Lee D.S., Walton Z.E., Ochocki J.D., Mathew L.K., Mancuso A., Gade T.P., Keith B., Nissim I, & Simon M.C. (2014). Fructose-1, 6-bisphosphatase opposes renal carcinoma progression. Nature, 513(7517), 251-255.
+ Open protocol
+ Expand
2

Mitochondrial Respiration Assay in RCC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
RCC4 or RCC10 cells ectopically expressing vector or FBP1 were seeded into black opaque 96-well plate at a density of 70, 000 cells per well. After attachment, culture medium within each well was replaced with 150 µL oxygenated fresh medium, supplemented with 10 µL MitoXpess probe solution (Cayman Chemical). Antimycin A was used as negative control. 100 µL mineral oil was dispensed to overlay each well, and the whole plate was monitored using SpectraMax m2e microplate reader (Molecular Devices) at 37 °C. Wavelengths are set to 380 nm for excitation and 650 nm for emission.
Li B., Qiu B., Lee D.S., Walton Z.E., Ochocki J.D., Mathew L.K., Mancuso A., Gade T.P., Keith B., Nissim I, & Simon M.C. (2014). Fructose-1, 6-bisphosphatase opposes renal carcinoma progression. Nature, 513(7517), 251-255.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!