Hsc 1

Manufactured by Japanese Collection of Research Bioresources Cell Bank

Sourced in Japan

The HSC-1 is a laboratory equipment designed for the culture and maintenance of human stem cells. It provides a controlled environment for the growth and propagation of these specialized cells. The core function of the HSC-1 is to facilitate the in vitro culture of human stem cells, enabling researchers to study their properties and potential applications.

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Lab products found in correlation

Hsc 5, by Japanese Collection of Research Bioresources Cell Bank (3 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Phospho jnk antibody, by Santa Cruz Biotechnology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Phospho s6k, by Cell Signaling Technology (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) A431 cells, by American Type Culture Collection (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Hacat, by CLS Cell Lines Service (1 mentions) Qiaamp dna mini kit, by Qiagen (1 mentions) Isogen, by Nippon Gene (1 mentions) Hek293t, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Pcdna3, by Addgene (1 mentions) Hacat, by Cytion (1 mentions) Si timp3, by GenePharma (1 mentions) Penicillin, by Sangon (1 mentions) Streptomycin, by Sangon (1 mentions)

7 protocols using hsc 1

Artocarpin Modulates Cell Signaling Pathways

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The human cutaneous SCC cell line HSC-1 was obtained from Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) and was cultured in Dulbecco's Modified Eagle's Medium with 20% FBS. Cells were incubated at 37°C with humidified air containing 5% CO₂.
Artocarpin was purchased from Pulin Biotech Company Limited (Taipei, Taiwan). The purity of artocarpin was determined to be greater than 98% by high performance liquid chromatography (HPLC) analysis. Phospho-ERK, phospho-p38, and phospho-S6K antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA); phospho-JNK antibody was purchased from Santa Cruz Biotechnology (Dallas, TX); phospho-Akt antibody was from Millipore (Billerica, MA, USA); phospho-mTOR and GAPDH antibodies were from Epitomics (Burlingame, CA, USA).

Hu S.C., Lin C.L., Cheng H.M., Chen G.S., Lee C.W, & Yen F.L. (2015). Artocarpin Induces Apoptosis in Human Cutaneous Squamous Cell Carcinoma HSC-1 Cells and Its Cytotoxic Activity Is Dependent on Protein-Nutrient Concentration. Evidence-based Complementary and Alternative Medicine : eCAM, 2015, 236159.

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C3a Stimulation and Inhibition in cSCC

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The cSCC cell lines HSC‐1 and HSC‐5 were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). A431 cells were obtained from the American Type Culture Collection (Manassas, VA, USA). SCC13 cells were kindly provided by Prof. Paolo Dotto of the Cutaneous Biology Research Center at Massachusetts General Hospital in Charlestown, MA, USA. Tca8113 cells were purchased from the China Center for Type Culture Collection (Wuhan, China). Cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL; Invitrogen, Carlsbad, CA, USA). Cells were grown under a humidified atmosphere of 5% CO₂ at 37°C.
A431 and SCC13 cells were exposed to a human C3a peptide agonist, as described in a previous study.22 A431 and SCC13 cells were treated with 0.1 and 0.2 μmol/L of C3a respectively, for 24 hours. To block C3aR activity, A431 and SCC13 cells were pretreated with the C3aR antagonist SB290157 (0.2 μmol/L) for 24 hours. The control group was treated with an equivalent volume of saline.

Fan Z., Qin J., Wang D, & Geng S. (2019). Complement C3a promotes proliferation, migration and stemness in cutaneous squamous cell carcinoma. Journal of Cellular and Molecular Medicine, 23(5), 3097-3107.

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Culturing Human SCC Cell Lines

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Human SCC cell lines (HSC-1, HSC-5) (25 (link),26 (link)) were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan), and the immortalized human keratinocyte cell line, HaCaT, was purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany). HSC-1 and HSC-5 cells were cultured in RPMI-1640 (Gibco, Grand island, NY, USA) medium supplemented with 10% heat-inactivated fetal bovine serum (FBS; Nichirei Biosciences Inc., Tokyo Japan), and HaCaT cells were cultivated in DMEM medium supplemented with 10% FBS, at 37°C under a humidified 5% CO₂ atmosphere.

KANZAKI A., KUDO M., ANSAI S.I., PENG W.X., ISHINO K., YAMAMOTO T., WADA R., FUJII T., TEDUKA K., KAWAHARA K., KAWAMOTO Y., KITAMURA T., KAWANA S., SAEKI H, & NAITO Z. (2016). Insulin-like growth factor 2 mRNA-binding protein-3 as a marker for distinguishing between cutaneous squamous cell carcinoma and keratoacanthoma. International Journal of Oncology, 48(3), 1007-1015.

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Sourcing and Preparation of SCC and Normal Skin Samples

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SCC cell lines HSC-1 and HSC-5 were provided from the Japanese Collection of Research Bioresources (Tokyo, Japan). SCC cell lines A431 and DJM-1 were provided from the Riken BioResources Center (Tsukuba, Japan). SCC cell line A388 was purchased from the American Type Culture Collection (Manassas, VA). Two normal human epidermal keratinocytes (NHEKs) derived from an adult and a neonate were obtained from ScienCell Research Laboratories (Carlsbad, CA). Eighteen paraffin-embedded SCC samples and 21 paraffin-embedded KA samples were obtained from each patient (Tables 1 and 2). Seven normal skin samples were obtained by shaving the margin of the excised epidermal cysts (Table 3). The TNM classification of SCC was evaluated according to the Union for International Cancer Control TNM Classification of Malignant Tumours (7^th edition). The diagnoses of SCC, KA, and normal skin were made histopathologically by at least two experienced board-certified pathologists. To extract DNA from paraffin-embedded samples, the samples were sliced into 4–10 μm–thick sections, deparaffinized, and then dissected with a fine needle. Genomic DNA was extracted by using the QIAamp DNA mini kit (Qiagen, Valencia, CA). Total RNA was isolated by using ISOGEN (Nippon Gene, Tokyo, Japan).

Nobeyama Y, & Nakagawa H. (2016). Aberrant DNA Methylation in Keratoacanthoma. PLoS ONE, 11(10), e0165370.

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HSC-1 Human Squamous Cell Carcinoma Culture

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Human skin cell line HSC-1, a derivative of squamous cell carcinoma, was obtained from the JCRB Cell Bank. HSC-1 cells were cultured in DMEM high glucose (Hyclone, Grand island, NY, USA) supplemented with 20% fetal bovine serum and 1% penicillin/streptomycin. Cells were maintained in a humidified incubator at 37 °C with 5% CO₂.

León D., Buchegger K., Silva R., Riquelme I., Viscarra T., Mora-Lagos B., Zanella L., Schafer F., Kurachi C., Roa J.C., Ili C, & Brebi P. (2020). Epigallocatechin Gallate Enhances MAL-PDT Cytotoxic Effect on PDT-Resistant Skin Cancer Squamous Cells. International Journal of Molecular Sciences, 21(9), 3327.

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Cell Line Cultivation and Transfection

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CSCC cell lines HSC-1 (JCRB Cell Bank, Osaka, Japan) and A431 (ATCC, VA, USA), human immortalized keratinocytes HaCaT (Cell Lines Service, Eppelheim, Germany) and human embryo kidney epithelial cell HEK293T (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS), 100 µg/mL streptomycin and 100 units/mL penicillin (Sangon, Shanghai, China). MiR-21 inhibitor, si-TIMP3 and the corresponding negative controls (NCs) were synthesized by Genepharma (Shanghai, China). pcDNA 3.1 (Addgene, Cambridge, MA, USA) was adopted to construct low expression vectors. Lipofectamine 3000 was mixed with DNA at 1:1 in Opti-MEM and added into cells for transfection. At 2 days post-transfection, the cells were analyzed.

Yin S, & Lin X. (2021). MicroRNA-21 Contributes to Cutaneous Squamous Cell Carcinoma Progression via Mediating TIMP3/PI3K/AKT Signaling Axis. International Journal of General Medicine, 14, 27-39.

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Establishment of MAGEA12 Knockdown cSCC Cell Lines

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Two human cSCC cell lines, HSC-1 and A431, were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) and the Korean Cell Line Bank (Seoul, Korea), respectively, for use in this study. Cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco Biosciences, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen, Waltham, MA, USA). pGFP-C-shMAGEA12 lentiviral particle (OriGene, Rockville, MD, USA) was used to establish HSC-1 and A431 cells with stable MAGEA12 knockdown (HSC-1-MAGEA12 Δ and A431-MAGEA12 Δ , respectively). Silencing of MAGEA12 was confirmed with immunocytochemistry. Lentiviral particles for pGFP-C-shLenti vector weve used to establish control stable cell lines (HSC-1-Mock and A431-Mock).

Zhao G., Bae J.Y., Zheng Z., Park H.S., Chung K.Y., Roh M.R, & Jin Z. (2019). Overexpression and Implications of Melanoma-associated Antigen A12 in Pathogenesis of Human Cutaneous Squamous Cell Carcinoma. Anticancer research, 39(4).

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