Log In Sign up
The largest database of trusted experimental protocols

Tranist tko transfection reagent

Manufactured by Mirus Bio
Visit Supplier

Tranist-TKO is a transfection reagent designed for the efficient delivery of genetic material, such as plasmids, siRNA, and mRNA, into a variety of cell types. It facilitates the uptake of these molecules by the target cells, enabling various applications in cell biology research and gene expression studies.

Automatically generated - may contain errors

Lab products found in correlation

Modulas microplate luminometer, by Promega (2 mentions) Miridian microrna mimic, by GE Healthcare (2 mentions) Dual luciferase reporter assay, by Promega (2 mentions)

Spelling variants of this product

Transfection reagent (10 citations) TKO transfection reagent (4 citations)

2 protocols using tranist tko transfection reagent

1

Evaluating miR-122-5p binding to ALDOA 3'UTR

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ALDOA 3′UTR fragment was cloned into the psi-CHECK2 dual-luciferase reporter system between XhoI and SpeI restriction sites using the primers found in Supplementary Table 7. A miRIDIAN microRNA mimic for miR-122-5p was obtained for the 22nt isoform or custom synthesized for the 21nt isoform (GE Healthcare). For transfection, 250 ng of psi-CHECK2 reporter plasmids were co-transfected with a final concentration of 25 nM of synthetic miRNA mimics in 24-well plates of HEK293 cells using the Tranist-TKO transfection reagent (Mirus). Cells were lysed in 1× Passive Lysis Buffer and evaluated using a Dual Luciferase Reporter Assay (Promega) 24 hours after transfection, detected on a Modulas Microplate Luminometer (Turner Biosystems).
Valdmanis P.N., Gu S., Chu K., Jin L., Zhang F., Munding E.M., Zhang Y., Huang Y., Kutay H., Ghoshal K., Lisowski L, & Kay M.A. (2016). RNAi induced hepatotoxicity results from loss of the first synthesized isoform of miR-122 in mice. Nature medicine, 22(5), 557-562.
+ Open protocol
+ Expand
2

Evaluating miR-122-5p binding to ALDOA 3'UTR

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ALDOA 3′UTR fragment was cloned into the psi-CHECK2 dual-luciferase reporter system between XhoI and SpeI restriction sites using the primers found in Supplementary Table 7. A miRIDIAN microRNA mimic for miR-122-5p was obtained for the 22nt isoform or custom synthesized for the 21nt isoform (GE Healthcare). For transfection, 250 ng of psi-CHECK2 reporter plasmids were co-transfected with a final concentration of 25 nM of synthetic miRNA mimics in 24-well plates of HEK293 cells using the Tranist-TKO transfection reagent (Mirus). Cells were lysed in 1× Passive Lysis Buffer and evaluated using a Dual Luciferase Reporter Assay (Promega) 24 hours after transfection, detected on a Modulas Microplate Luminometer (Turner Biosystems).
Valdmanis P.N., Gu S., Chu K., Jin L., Zhang F., Munding E.M., Zhang Y., Huang Y., Kutay H., Ghoshal K., Lisowski L, & Kay M.A. (2016). RNAi induced hepatotoxicity results from loss of the first synthesized isoform of miR-122 in mice. Nature medicine, 22(5), 557-562.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!