Poly 1 c potassium salt

Manufactured by Merck Group

Sourced in United States

Poly(I:C) potassium salt is a synthetic double-stranded RNA compound used as a tool in biological research. It functions as a potent inducer of type I interferon response, which can be utilized to study innate immune system activation. The product is supplied as a powder form.

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Poly(I:C) (527 citations) Poly(I) (6 citations) Poly-I-poly-C (5 citations)

4 protocols using poly 1 c potassium salt

Maternal Immune Activation Mouse Model

Cited 2 times

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MIA was induced using the viral mimic poly(I:C) as described in an earlier study (Garay et al, 2013 (link)). Briefly, pregnant mice were injected intraperitoneally (i.p.) on E12.5 with saline or poly(I:C) potassium salt (Sigma Aldrich; St Louis, MO). E12.5 was chosen since this stage of gestation correlates with the late first trimester in humans (Clancy et al, 2007 (link)) the time that infections are most closely linked to increased incidence of SZ and ASD (Atladottir et al, 2010 (link)). Poly(I:C) was freshly dissolved in saline and administered i.p. at 20 mg/kg based on the weight of the poly(I:C) itself, not including the total weight of the potassium salts. Control mice were injected with saline alone. This concentration of poly(I:C) is higher than that used for intravenous injections (Meyer et al, 2006 (link)) and was selected because it is the optimal i.p. dose that causes MIA, while preserving viability of offspring (Ito et al, 2010 (link)). Pups remained with the mother until weaning on postnatal day (P) 21, at which time mice were group housed.

Pendyala G., Chou S., Jung Y., Coiro P., Spartz E., Padmashri R., Li M, & Dunaevsky A. (2017). Maternal Immune Activation Causes Behavioral Impairments and Altered Cerebellar Cytokine and Synaptic Protein Expression. Neuropsychopharmacology, 42(7), 1435-1446.

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Poly(I:C)-induced neuroinflammation in mice

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C57BL/6 mice (7 weeks old, male) were obtained from the Laboratory Animal Center of Huazhong Agricultural University, Wuhan, China. Mice were reared about 1 week for adaptation and randomly divide into control and treatment group (n = 9). Poly(I:C) potassium salt (Sigma-Aldrich, St. Louis, MO, USA) was diluted in normal saline (NS) at the concentration of 1 µg/µl. Mice were then treated with 100 µl NS and 100 µg of poly(I:C)/mouse respectively through intraperitoneal injection for 24 h. The mice were sacrificed after treatment period to collect brain and lung tissues following institutional guideline. The collected tissues of both the control and treated mice were subjected to RNA extraction.

Ali M.I., Li L., Li L., Yao L., Liu J., Gu W., Huang S., Wang B, & Liu G. (2020). The tissue specific regulation of miR22 expression in the lung and brain by ribosomal protein L29. Scientific Reports, 10, 16242.

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Maternal Immune Activation in Mice

Cited 1 time

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All protocols were approved by the University of Nebraska Medical Center Institutional Animal Care and Use Committee. YFP-H C57Bl/6J pregnant females were bred at UNMC facility with a 12:12 h light:dark cycle with food and water available ad libitum. Mice were mated overnight, and the presence of a vaginal plug on the following morning was noted as embryonic day (E)0.5. Pregnant mice were injected intraperitoneally (I.P.) on E12.5 with saline or 20 mg/Kg Poly (I:C) potassium salt (Sigma Aldrich; St. Louis. MO) (Malkova et al., 2012 (link)). Pups were allowed to be born naturally. Offspring of saline injected mice are referred to as control offspring and offspring of Poly(I:C) injected mice are referred to as MIA offspring. 3–6 litters from each condition were used for all experiments. With the exception of the behavioral data (where 2–5 mice were used per litter), in all other experiments no more than 1–3 pups were used from each litter. During data collection and analysis the experimenters were blind to the experimental conditions.

Coiro P., Padmashri R., Suresh A., Spartz E., Pendyala G., Chou S., Jung Y., Meays B., Roy S., Gautam N., Alnouti Y., Li M, & Dunaevsky A. (2015). Impaired synaptic development in a maternal immune activation mouse model of neurodevelopmental disorders. Brain, behavior, and immunity, 50, 249-258.

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Maternal Immune Activation in Mice

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Mice were mated overnight and the presence of a vaginal plug was designated as embryonic day 0.5 (E0.5). Each pregnant dam was weighed and administered 20 mg/kg PolyI:C potassium salt (Sigma Aldrich) or saline by intraperitoneal (i.p.) injections on both E11.5 and E12.5. Pups were weaned from their mothers at post-natal day 21 (P21) and housed with same sex littermates with 2-5 mice per cage.

Lammert C.R., Frost E.L., Bolte A.C., Paysour M.J., Shaw M.E., Bellinger C.E., Weigel T.K., Zunder E.R, & Lukens J.R. (2018). Critical roles for microbiota-mediated regulation of the immune system in a prenatal immune activation model of autism. Journal of immunology (Baltimore, Md. : 1950), 201(3), 845-850.

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