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Prl renilla luciferase reporter vector

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The PRL Renilla Luciferase Reporter Vector is a plasmid that expresses the Renilla luciferase reporter gene. Renilla luciferase is an enzyme that catalyzes the oxidation of a substrate, resulting in the emission of light. This vector can be used to monitor gene expression or other cellular processes in a variety of cell types.

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Lab products found in correlation

Dual luciferase reporter assay system, by Promega (5 mentions) Pgl4.10 luc2 vector, by Promega (2 mentions) Turbofect transfection reagent, by Thermo Fisher Scientific (2 mentions) Turbofect kit, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay system kit, by Promega (1 mentions)

Close spelling variants of this product

PRL-SV40 Renilla luciferase reporter vector PRL-TK Renilla luciferase control reporter vector PRL-SV40 Renilla luciferase control reporter vector PRL-TK Renilla luciferase-based reporter control vector PRL-CMV Renilla Luciferase Reporter Vector PRL-TK Renilla luciferase reporter vector PRL Renilla Luciferase Control Reporter Vector PRL-CMV Renilla luciferase control reporter vector PRL-TK vector (Renilla luciferase control reporter vector) PRL Renilla luciferase vector

8 protocols using prl renilla luciferase reporter vector

1

RXR Activation Assay in A549 and MDA-MB-435 Cells

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The A549 and MDA-MB-435 cells were cotransfected with CRBPII-tk-luc (a gift from makishima.makoto, Nihon University School of Medicine, JAPAN), pCMX-hRXR, and pRL Renilla Luciferase Reporter Vector (Promega Corporation). The ratio of these three plasmids is 10: 3: 2. Five hours after transfection, the cells were treated with different concentrations of DW-22 and Bexarotene. After 48 h transfection, the RXR activity was detected with Dual-Luciferase Reporter Assay System (Promega Corporation). RXR activity is presented as the means ± SD of three determinants.
Wang L., Chen G., Chen K., Ren Y., Li H., Jiang X., Jia L., Fu S., Li Y., Liu X., Wang S., Yang J, & Wu C. (2015). Dual targeting of retinoid X receptor and histone deacetylase with DW22 as a novel antitumor approach. Oncotarget, 6(12), 9740-9755.
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2

Hsd17b1 Deletion and Naglu Expression

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To determine whether the deletion of the Hsd17b1 gene affects Naglu expression in vitro, we generated two expression constructs in pACYC177 plasmid backbone (New England Biolabs, Ipswich, MA, USA) of this genomic region with and without the genomic fragment spanning the 1.8 kb coding region of Hsd17b1 (Fig. 1F and G). The constructs were co-transfected with a luciferase-expressing reporter plasmid (pRL Renilla luciferase reporter vector, Promega, Madison, WI, USA) into COS cells using TurboFect kit (Thermo Fisher Scientific, Wilmington, DE, USA) according to the instructions supplied by the manufacturer. Thereafter, the expression of Naglu in the transfected cells was analysed by qRT-PCR and normalised to luciferase expression (n = 6).
Jokela H., Hakkarainen J., Kätkänaho L., Pakarinen P., Ruohonen S.T., Tena-Sempere M., Zhang F.P, & Poutanen M. (2017). Deleting the mouse Hsd17b1 gene results in a hypomorphic Naglu allele and a phenotype mimicking a lysosomal storage disease. Scientific Reports, 7, 16406.
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3

Melanoma Cell Transfection and Luciferase Assay

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1×104 melanoma cells were seeded per well in 96-well plates, and co-transfected using TurboFect Transfection Reagent (Thermo Scientific, Waltham, MA, USA) with pCEACAM1 constructs or an empty pGL4.14 vector, and pcDNA3/SOX9 or an empty pcDNA3 vector and pRL Renilla luciferase reporter vector (Promega, Madison, WI, USA). After 48 hours, cells were lysed and firefly luciferase activity was measured with Dual Luciferase Reporter Assay System (Promega, Madison, WI, USA) and normalized to Renilla.
Ashkenazi S., Ortenberg R., Besser M., Schachter J, & Markel G. (2016). SOX9 indirectly regulates CEACAM1 expression and immune resistance in melanoma cells. Oncotarget, 7(21), 30166-30177.
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4

Evaluating CycT1 Mutant Effects on HIV Transcription

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Tat-independent inhibitory effects of HA-CycT1 mutants were monitored by transiently co-transfecting pHAGE-HA-CycT1 mutant (2 μg) and the HIV-LTR-luciferase reporter (0.1 μg). pRL Renilla Luciferase Reporter Vector (Promega) (200 ng/24well) was used to measure transfection efficiencies and luciferase readings were normalized to the Renilla expression.
For analysis of inhibition of Tat-dependent transactivation pHAGE-HA-CycT1 mutants were transfected (2-4 μg) with the LTR-Tat-BFP lentiviral vector (1 μg). pCDNA3-CMV-GFP (0.1 μg) was also transfected into cells, to monitor transfection efficiencies 48 hr. post transfection, cells were harvested and their BFP expression were measured by FACS relatively to cells that expressed wild type HA-CycT1. Data were presented as (−) or (+) representing inhibitory affects on HIV transcription in human cells.
For effects of HA-CycT1 mutants on the NF-kB or CMV promoters, HA-CycT1 mutants were transfected with into human HEK-293T cells (2-4 μg) together with either the NF-kB-Luciferase promoter or the CMV-BFP promoter (1 μg). Cells were harvested 48 hr. post transfection and analyzed for their luciferase activity or BFP expression by FACS. Data are presented relatively to effects of CycT1-wild type –set to 100.
Kuzmina A., Verstraete N., Galker S., Maatook M., Bensaude O, & Taube R. (2014). A single point mutation in cyclin T1 eliminates binding to Hexim1, Cdk9 and RNA but not to AFF4 and enforces repression of HIV transcription. Retrovirology, 11, 51.
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5

Regulation of CEACAM1 Promoter Activity

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To measure the effect of BRAF or MEK inhibitors on CEACAM1 promoter activity, pGL4.14 empty vector, CEACAM1p or CEACAM1p-ΔETS1 constructs were co-transfected with pRL Renilla Luciferase Reporter Vector (Promega, Madison, WI) into melanoma cells in a 50:1 ratio using TurboFect Transfection Reagent (Fermentas, Burlington, Canada) according to manufacturer’s instructions. Cells were incubated with 1 μM of vemurafenib or selumetinib for 48 hours. After 48 hours, cells were lysed, and luciferase activity was measured. To measure the effect of ETS1 on CEACAM1 promoter activity, 293T cells were co-transfected with 10 ng pGL4.14 empty vector or CEACAM1p, together with 100 ng ETS1 or ETS1T38A or mock (pQCXIP), along with 0.4 ng pRL Renilla Luciferase Reporter Vector using TurboFect Transfection Reagent according to manufacturer’s instructions. After 48 hours, cells were lysed and luciferase activity was measured. All assays were measured by Dual Luciferase Reporter Assay System (Promega) using GlowMarx microplate reader (Promega) and normalized to the Renilla signal.
Kfir-Elirachman K., Ortenberg R., Vizel B., Besser M.J., Barshack I., Schachter J., Nemlich Y, & Markel G. (2018). Regulation of CEACAM1 Protein Expression by the Transcription Factor ETS-1 in BRAF-Mutant Human Metastatic Melanoma Cells. Neoplasia (New York, N.Y.), 20(4), 401-409.
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6

Dual Luciferase Reporter Assay for Gene Silencing

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Briefly, 1×106 HeLa cells were co-transfected with 30 pmol siRNAs, 1 μg pGL3 Firefly luciferase plasmids and 100 ng pRL Renilla luciferase reporter vector (Promega, E2261). After transfection for 48 h, cells were lysed with passive lysis buffer on ice for 20 min and the luciferase activity was performed with Dual Luciferase Reporter Assay System Kit (Promega, E1910) according to the manufacturer’s protocol. The Firefly luciferase activities were measured and normalized to Renilla luciferase activities (F/R).
Li X., Li J., Shan G, & Wang X. (2023). Identification of long non-coding RNA and circular RNA associated networks in cellular stress responses. Frontiers in Genetics, 14, 1097571.
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7

Characterizing c-MYB Transcriptional Activity

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Luciferase reporter constructs containing a consensus DNA-binding sequence for c-MYB were generated. The reporter construct was designed using the core MYB recognition element (MRE) consensus sequence PyAAC(G/T)G which is present in the mim-1 gene promoter, a previously described MYB target21 . Double stranded oligos were generated by annealing primers mim-1 forward and mim-1 reverse (supplementary Table 9). The annealed oligo was ligated into pGL4.10[luc2] vector (Promega) digested with XhoI and HindIII. The pRL Renilla Luciferase Reporter Vector (Promega E2261) served as an internal control in all assays. The mim-1 reporter construct and pRL renilla vector (ratio 30:1) were co-transfected into HEK-293 along with indicated fusions or controls via Lipofectamine 2000. Luciferase activity was quantified 24 hours post transfection using the Dual-Luciferase Reporter Assay System (Promega) according to manufacturer’s protocol.
Bandopadhayay P., Ramkissoon L.A., Jain P., Bergthold G., Wala J., Zeid R., Schumacher S.E., Urbanski L., O’Rourke R., Gibson W.J., Pelton K., Ramkissoon S.H., Han H.J., Zhu Y., Choudhari N., Silva A., Boucher K., Henn R.E., Kang Y.J., Knoff D., Paolella B.R., Gladden-Young A., Varlet P., Pages M., Horowitz P.M., Federation A., Malkin H., Tracy A., Seepo S., Ducar M., Hummelen P.V., Santi M., Buccoliero A.M., Scagnet M., Bowers D.C., Giannini C., Puget S., Hawkins C., Tabori U., Klekner A., Bognar L., Burger P.C., Eberhart C., Rodriguez F.J., Hill D.A., Mueller S., Haas-Kogan D.A., Phillips J.J., Santagata S., Stiles C.D., Bradner J.E., Jabado N., Goren A., Grill J., Ligon A.H., Goumnerova L., Waanders A.J., Storm P.B., Kieran M.W., Ligon K.L., Beroukhim R, & Resnick A.C. (2016). MYB-QKI rearrangements in Angiocentric Glioma drive tumorigenicity through a tripartite mechanism. Nature genetics, 48(3), 273-282.
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8

Characterizing c-MYB Transcriptional Activity

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Luciferase reporter constructs containing a consensus DNA-binding sequence for c-MYB were generated. The reporter construct was designed using the core MYB recognition element (MRE) consensus sequence PyAAC(G/T)G which is present in the mim-1 gene promoter, a previously described MYB target21 . Double stranded oligos were generated by annealing primers mim-1 forward and mim-1 reverse (supplementary Table 9). The annealed oligo was ligated into pGL4.10[luc2] vector (Promega) digested with XhoI and HindIII. The pRL Renilla Luciferase Reporter Vector (Promega E2261) served as an internal control in all assays. The mim-1 reporter construct and pRL renilla vector (ratio 30:1) were co-transfected into HEK-293 along with indicated fusions or controls via Lipofectamine 2000. Luciferase activity was quantified 24 hours post transfection using the Dual-Luciferase Reporter Assay System (Promega) according to manufacturer’s protocol.
Bandopadhayay P., Ramkissoon L.A., Jain P., Bergthold G., Wala J., Zeid R., Schumacher S.E., Urbanski L., O’Rourke R., Gibson W.J., Pelton K., Ramkissoon S.H., Han H.J., Zhu Y., Choudhari N., Silva A., Boucher K., Henn R.E., Kang Y.J., Knoff D., Paolella B.R., Gladden-Young A., Varlet P., Pages M., Horowitz P.M., Federation A., Malkin H., Tracy A., Seepo S., Ducar M., Hummelen P.V., Santi M., Buccoliero A.M., Scagnet M., Bowers D.C., Giannini C., Puget S., Hawkins C., Tabori U., Klekner A., Bognar L., Burger P.C., Eberhart C., Rodriguez F.J., Hill D.A., Mueller S., Haas-Kogan D.A., Phillips J.J., Santagata S., Stiles C.D., Bradner J.E., Jabado N., Goren A., Grill J., Ligon A.H., Goumnerova L., Waanders A.J., Storm P.B., Kieran M.W., Ligon K.L., Beroukhim R, & Resnick A.C. (2016). MYB-QKI rearrangements in Angiocentric Glioma drive tumorigenicity through a tripartite mechanism. Nature genetics, 48(3), 273-282.
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