B16F10 tumour tissues and lung tissues were fixed in 4% paraformaldehyde before being embedded in Optimal Cutting Temperature compound. Immunofluorescence staining was performed using rat anti-mouse CD31 antibody (BD Biosciences), biotinylated anti-rat IgG as a secondary antibody and Texas Red Avidin DCS (Vector Laboratories, Inc.). Cryosections (Leica cryostat) were mounted with Vectashield containing DAPI and were visualized with an ultraviolet fluorescent microscope (Nikon Eclipse E800) with a Retiga camera (QImaging) through IPLab version 3.65a imaging software (Scanalytics).
Texas red avidin dcs
Manufactured by Vector Laboratories
Sourced in United States
Texas Red avidin DCS is a conjugate of the protein avidin and the fluorescent dye Texas Red. Avidin is a glycoprotein that binds strongly to the vitamin biotin. Texas Red is a fluorescent compound that can be excited with light at 595 nm and emits light at 615 nm. The Texas Red avidin DCS conjugate retains the biotin-binding properties of avidin and the fluorescent properties of Texas Red, which allows it to be used as a detection reagent in various bioanalytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Biotinylated anti avidin d, by Vector Laboratories (4 mentions)
Axioplan 2 microscope, by Zeiss (3 mentions)
Biotin 14 dutp, by Thermo Fisher Scientific (3 mentions)
Metavuetm, by Universal Imaging (3 mentions)
Vectashield, by Vector Laboratories (2 mentions)
Rat anti mouse cd31 antibody, by BD (1 mentions)
Dmrxa2 fluorescence microscope, by Leica Microsystems (1 mentions)
Volocity, by PerkinElmer (1 mentions)
Biotin dutp, by Roche (1 mentions)
Alexa 488 5 dutp, by Thermo Fisher Scientific (1 mentions)
5 protocols using texas red avidin dcs
1
Immunofluorescence Analysis of Tumor Angiogenesis
2
Chromosome Identification via BAC-FISH
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Preparation of slides and hybridization using bacterial artificial chromosome—fluorescent in situ hybridization (BAC-FISH) was carried as described in [53 (link)–55 (link)]. A B. distachyon BAC clone, ABR1-63-E6, that hybridizes all chromosomes of B. distachyon, but not those of B. stacei was labelled by random priming with biotin-14-dUTP (Invitrogen, Life Technologies) [56 (link)]. A ribosomal DNA probe, pTa 71,[57 (link)] which contains a 9-kb EcoRI fragment of rDNA repeat unit (18S-5.8S-26S genes and spacers) isolated from Triticum aestivum was labelled with Alexa-488 dUTP by random priming. Biotinylated probe was immunodetected using Texas Red avidin DCS (Vector Laboratories) and the signal was amplified with biotinylated anti-avidin D (Vector Laboratories). The chromosomes were mounted and counterstained in Vectashield (Vector Laboratories) containing 2.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI). Fluorescence images were captured using a CoolSnap HQ camera (Photometrics, Tucson, Ariz) on an Axioplan 2 microscope (Zeiss, Oberkochen, Germany) and analysed using MetaVueTM (Universal Imaging Corporation,Downington, PA).
3
Chromosome Preparation and In Situ Hybridization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chromosome preparations and in situ hybridization were performed as described in D’Hont et al. (2000 ) with modifications. BAC clones (Table S3 ) from accession DH‐Pahang (D’Hont et al., 2012 ; http://banana‐genome.cirad.fr ) were labeled by random priming with biotin‐14‐dUTP (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) or Alexa 488‐5‐dUTP (Life Technologies; Thermo Fisher Scientific). Chromosome preparations were incubated in RNAse A (100 ng µl–1) and pepsin (100 mg ml–1) in 0.01 m HCl and fixed with paraformaldehyde (4%). Biotinylated probes were immunodetected by Texas Red avidin DCS (Vector Laboratories, Burlingame, CA, USA) and the signal was amplified with biotinylated antiavidin D (Vector Laboratories). Fluorescence images were captured using a cooled high‐resolution black and white CCD camera (ORCA; Hamamatsu, Hamamatsu City, Japan) fitted on a DMRXA2 fluorescence microscope (Leica Microsystems, Wetzlar, Germany) and analysed using volocity (Perkin Elmer, Waltham, MA, USA).
4
Fluorescent in-situ Hybridization of Brassica Genomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Preparation of slides and hybridization were carried out according to procedures detailed by Suay et al. (2014) (link). The ribosomal probe used in this study was 35S rDNA (pTa 71 clone) from wheat (Gerlach and Bedbrook, 1979 (link)), IGS-A and IGS-C probes described further below and the BAC clone B. oleracea named Bob014O06 (Howell et al., 2002 (link)). This BAC clone was used as ‘GISH-like’ to distinguish specifically all C-genome chromosomes in B. napus (Suay et al., 2014 (link)). The 35S rDNA and BAC clone were labelled with Alexa-488 dUTP by random priming, the IGS-A with biotin-dUTP (Roche, Mannheim, Germany) using PCR and the IGS-C with biotin-dUTP (Roche) using nick translation (Bionick DNA labelling System, Thermo Fisher Scientific, Waltham, MA, USA). Biotinylated probe was immunodetected by Texas Red avidin DCS (Vector Laboratories, Burlingame, CA, USA) and the signal was amplified with biotinylated anti-avidin D (Vector Laboratories). The chromosomes were mounted and counterstained in Vectashield (Vector Laboratories, Ontario, Canada) containing 2·5 μg mL–1 4′,6-diamidino-2-phenylindole (DAPI). Fluorescence images were captured using a CoolSnap HQ camera (Photometrics, Tucson, AZ, USA) on an Axioplan 2 microscope (Zeiss, Oberkochen, Germany) and analysed using MetaVueTM (Universal Imaging Corporation, Downington, PA, USA).
5
Chromosome-based BAC Localization in Banana
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chromosome preparations were performed as described in D’Hont et al. (2000) . Seven BAC clones (MAMB_34N11, MAMB_17B03, MAMB_51M04, MAMH_47D06, MAMB_01M16, MAMB_51J24, and MAMH_66D03) from both sides of the breakpoints were selected from a BamH1 and HindIII BAC libraries of accession DH-Pahang (D’Hont et al. 2012 (link); http://banana-genome.cirad.fr/ ; last accessed May 29, 2017). BAC clones were labeled by random priming with biotin-14-dUTP (Invitrogen, Life Technologies) or Alexa 488-5-dUTP (Invitrogen, Life Technologies). In situ hybridization was performed as described in D’Hont et al. (1996) (link) with the following modifications. Chromosome preparations were incubated in RNAse A (100 ng/µl), pepsin (100 mg/ml) in 0.01M HCl and fixed with paraformaldehyde (4%). Biotinylated probes were immunodetected by Texas Red avidin DCS (Vector Laboratories) and the signal was amplified with biotinylated antiavidin D (Vector Laboratories). Fluorescence images were captured using a CoolSnap HQ camera (Photometrics, Tucson, Ariz) via an Axioplan 2 microscope (Zeiss, Oberkochen, Germany) and analyzed using MetaVueTM (Universal Imaging Corporation, Downington, PA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!