The largest database of trusted experimental protocols

Su 8 2005

Manufactured by MicroChem
Sourced in United States

SU-8 2005 is a negative-tone photoresist material developed for microfabrication applications. It is a high-contrast, epoxy-based photoresist designed for use in ultraviolet (UV) photolithography processes. The material is sensitive to near-UV and UV radiation, allowing for the creation of high-aspect-ratio structures. SU-8 2005 is commonly used in the fabrication of microelectromechanical systems (MEMS), microfluidic devices, and other microfabricated components.

Automatically generated - may contain errors

19 protocols using su 8 2005

1

Fabrication of Recessed Microelectrodes

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the electrodes are fabricated using a similar method as previously reported12 (link). We first sputtered a release layer of 70 nm Al on a Si wafer (University Wafer). Followed by photolithography to pattern the bottom SU8: spin coated SU8 2005 (MicroChem) at 5000 rpm for 30 s, resulting in a 4 μm thick layer, then prebaked at 65 °C and 95 °C for 1 min and 4 min, respectively. After exposure, the wafer was post-baked again at 65 °C and 95 °C for 1 min and 4 min, respectively, then developed in SU8 developer for 2 min. Next, LOR 3A was spin-coated at 2000 rpm for 1 min, baked at 100 °C and 150 °C for 2 min and 10 min as lift-off sacrificial layer. Then S1818 was spin-coated at 4000 rpm for 1 min, baked at 115 °C for 1 min. After exposure, the wafer was developed in MF321 for 2 min. Prior to sputtering metal traces, the surface was treated with oxygen plasma at 100 W for 1 min to enhance adhesion. A 100 nm thick Pt layer was sputtered and lifted off with acetone. For gold electrodes, 8 μm-thick Ti was sputtered as an adhesion layer. Next, top insulation SU8 was patterned using the same steps as bottom SU8. For recessed electrodes, a dry etch was applied using Reactive Ion Etcher (Oxford 180) to open up the top SU8 vias for small electrodes. Before releasing, the microelectrodes were hardbaked at 180 °C for 1 hour. The microelectrodes were released using MF 321 for 8 hours.
+ Open protocol
+ Expand
2

Fabrication of Microfluidic Chips with Electrodes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two types of chips were fabricated and used in this work: chips with coplanar electrodes and chips with front facing electrodes (Figure 2B). The fabrication process is similar for the two chip types: Gold electrodes were defined on 4-inch Pyrex wafers by photolithography, e-beam vapor deposition and lift-off, using titanium as an adhesive layer. On top of the electrodes the channels were formed in SU-8 2005 (MicroChem, Berlin, Germany) by negative photolithography as described by Demierre et al. [27 (link),28 (link)]. The channels were sealed using a second Pyrex wafer (lid wafer) with openings for electrode access and fluidic inlet and outlet defined using powder blasting. The lid wafer used for the front facing electrodes had additionally gold electrodes fabricated as those of the bottom wafer. The lid wafer was then thermally bonded to the bottom wafer as described by Serra et al. [29 ]. The microchannels were 10 μm wide and 10 μm high. The front facing electrodes exposed to the channel are 10 μm long and 10 μm wide with a pitch of 16 µm. The dimensions and pitch are the same for the coplanar electrodes.
+ Open protocol
+ Expand
3

Fabrication of PDMS-based Microfluidics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetone, anhydrous ethanol and deionized water were purchased from commercial sources in the highest available purity. The negative photoresist SU-8 2005 and its developer were obtained from MicroChem Corp. Glass slides were used for the substrates. The elastomer PDMS Sylgard 184 was purchased from Dow Corning. The SiO2 coating agent was purchased from Changzhou Nanocoatings Co., Ltd.
+ Open protocol
+ Expand
4

Microfabricated Devices for Multi-Habitat Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols
Microfabricated devices used in this study consist of two inlet holes (1.2mm) on opposite sides with four parallel landscapes, each with 1-dimensional arrays of 85 habitat patches (100 × 100 × 5μm3) connected by corridors with constant lengths (50 μm) and depths (5 μm) but with different widths.
Devices were fabricated using soft lithography techniques (Qin et al., 2010 (link)): A silicon wafer was coated with a thin film (5 μm, height of the device) of the negative photoresist SU-8 (SU-8 2005, MicroChem) and the design of the device was written into the resist with a laser pattern generator (μPG 101, Heidelberg Instruments) to fabricate a master mold on which Polydimethylsiloxane (10:1 PDMS:curing agent, Sylgard 184, Dow Corning) was deposited to yield an elastomeric stamp that was covalently bonded to a glass cover slip by oxygen plasma activation (29.6 W, 400 mTorr, 45 s; PDC-002, Harrick Plasma) of both the PDMS and glass parts.
+ Open protocol
+ Expand
5

Inkjet-Printed Microfluidics Fabrication

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three different inks were involved in fabricating the fully inkjet-printing microfluidics or microfluidics-based sensors. The silver ink was SunTronic silver nanoparticle ink from Sun Chemical Corporation (Parsippany, USA), while PMMA ink and SU-8 ink were home-made. The PMMA ink was made by dissolving PMMA powder into a mixture of anisole and dimethyl sulfoxide (DMSO) with anisole dissolving the PMMA powder and DMSO optimizing the ink drop’s surface tension and viscosity. All these chemicals including PMMA, anisole and DMSO were from Sigma-Aldrich Corporation (St. Louis, USA). The SU-8 ink was a blend of SU-8 2002 and SU-8 2005 (Microchem Corporation, Westborough, USA). The ratio of two different viscosity SU-8 was carefully tuned to enable successful printing. All inks were pre-filtered through a 0.2 μm syringe filter to prevent potential nozzle clog. Ultrasonic cleaning bath (Branson 1510 ultrasonic cleaner, Danbury, USA) was used in both blending the inks and etching the channels.
+ Open protocol
+ Expand
6

Fabrication of PDMS Microfluidic Device

Check if the same lab product or an alternative is used in the 5 most similar protocols
Briefly, SU-8 2005 (MicroChem, USA) was used to develop the DLD device negative mould on a 4-inch silicon wafer at a height of 3 µm. The SU-8 was patterned using a hard chrome glass mask (Infinite Graphics, Singapore) on a SUSS-MA8 lithography mask aligner. The final device was fabricated using PDMS cured on the silicon SU-8 mould. The input and output holes were punched and the PDMS device was bonded onto a glass slide using a oxygen plasma treatment for 2 min in the March PX-250 plasma machine. All devices used in this work were fabricated from the same mould to ensure consistency in results.
+ Open protocol
+ Expand
7

SU8 Microchannel Master Fabrication

Check if the same lab product or an alternative is used in the 5 most similar protocols
SU8 microchannel masters were fabricated by photolithography by spincoating SU8 2005 (5.0 μm, MicroChem) onto a silicon wafer and exposing it with UV-light. Replicas of the masters were created using soft lithography. PDMS (Sylgard 184) was mixed (10:1 ratio of pre-polymer to Platinum catalyst) for 15 minutes with a hand held mixer (Kitchen Aid 9 speed mixer), poured onto the silicon master, and placed in a 65°C oven overnight. The PDMS replica was removed from the master and then made hydrophilic with oxygen plasma treatment (Diener Zepto System, 36s O2 plasma, 0.50 mbar pressure, 15% power) and stored in distilled water until ready to use.
+ Open protocol
+ Expand
8

Liquid Sample Encapsulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The liquid sample was made by diluting black Indian ink (Majuscule®) 10× in water to yield a ~2 wt% carbon concentration. The liquid was enclosed between two 300 µm-thick, 1 inch-diameter, glass substrates (Schott D263®). A photoresist (SU-8 2005, MicroChem®) was coated and developed on one of the substrate to form a ring-patterned spacer. The spacer had an outer diameter of 22 mm, an inner diameter of 19 mm, and a thickness of 10 µm to ensure separation between the substrates.
+ Open protocol
+ Expand
9

Fabrication of PDMS Microfluidic Devices

Check if the same lab product or an alternative is used in the 5 most similar protocols
PDMS devices were fabricated using standard multi-layer soft lithography techniques37 (link). Three different masks were drawn with AutoCAD: the barrier flow paths, the remaining chambers and the herringbone mixer structures. SU8-2005, SU8-2050 and SU8-2025 (MicroChem) were spin-coated on the same wafer to the heights of 5 μm, 75 μm, and 25 μm, respectively. Transparency mask features were transferred to the SU-8 coated wafer through standard UV photolithography. The wafers were then treated with tridecafluoro-1,1,2,2-tetrahydrooctyl-1-trichlorosilane (UCT Specialties, LLC) to facilitate the release of PDMS from the mold. PDMS (Sylgard 184, Dow Corning) was prepared by thoroughly mixing 10:1 ratio of base polymer to cross-linker ratio and degassing the mixture in a vacuum chamber for an hour. PDMS was poured onto the master and cured overnight at 90°C. After curing, the PDMS was removed from the master, cut, and shaped into its final form. Inlet and outlet access channels were punched with 18 gauge needles. The PDMS devices were plasma treated (PDC-32G plasma cleaner) and bonded onto glass coverslips.
+ Open protocol
+ Expand
10

Fabrication of Microfluidic Chambers

Check if the same lab product or an alternative is used in the 5 most similar protocols
We followed established protocols to fabricate microfluidic chambers (Cui et al., 2007 (link); Li et al., 2014 (link)). Briefly, masks were designed with AutoCAD (Autodesk). Masters consisting of 2 layers of photosensitive epoxy SU-8 (SU-8 2005 and SU-8 2010, respectively, from MicroChem) patterns were prepared by standard photolithography with a mask aligner (MJB4; Süss MicroTec) in a clean facility. The first layer of SU-8 (5 μm in depth) contained the microgrooves (5 μm in height; 5 μm in width), fabricated with a high-resolution chromium mask, whereas the second layer of SU-8 (100 μm in depth) contained the compartments and central flow channels, fabricated with a printed transparency mask. Replica molding of polydimethylsiloxane (PDMS; Dow Corning) was performed to obtain the elastic microfluidic chambers. The cell body and growth cone compartments were made using punchers (Harris Uni-Core; Ted Pella, Inc.) of 5 mm in diameter, and the inlet and outlet wells of the central flow channels were made using punchers of 3 mm in diameter.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!