Granzyme b

Manufactured by Bioss Antibodies

Granzyme B is a serine protease enzyme that is involved in the process of apoptosis, or programmed cell death. It plays a key role in the immune system's cytotoxic response, mediating the destruction of target cells by cytotoxic T lymphocytes and natural killer cells.

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Lab products found in correlation

Anti ifn γ, by ABclonal (1 mentions) Glypican 3, by Abcam (1 mentions) Ifn β, by Bioss Antibodies (1 mentions) Tunel detection kit, by Roche (1 mentions)

2 protocols using granzyme b

Immunohistochemical Analysis of Immune Markers

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Immunohistochemistry
(IHC) staining was carried out based on the previously described studies.^{31 (link),32 (link)} The primary antibody included anti IFN-γ (catalog number A12450,
1:100 dilution, ABclonal), Granzyme B (catalog number bs-1351R, 1:500
dilution, Bioss), and Ki-67 (catalog number GB13030-2, 1:1000 dilution,
Servicebio). After diaminobenzidine (#ZLI9107, ZSGB-BIO, Beijing,
China) staining and hematoxylin counterstaining, the tissue photos
were captured with an Olympus microscope (Olympus, Tokyo, Japan).

Guo W., Gao H., Li H., Ge S., Zhang F., Wang L., Shi H, & Han A. (2022). Self-Assembly of a Multifunction DNA Tetrahedron for Effective Delivery of Aptamer PL1 and Pcsk9 siRNA Potentiate Immune Checkpoint Therapy for Colorectal Cancer. ACS Applied Materials & Interfaces, 14(28), 31634-31644.

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Immunohistochemical Analysis of Tumor Specimens

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Individual tumor specimens were fixed in 10% of formalin and paraffin‐embedded. The tumor tissue sections (4 μm) were dewaxed, rehydrated, and subjected to antigen retrieval. The sections were incubated with specific primary antibodies against CD8 (Protintech, 1:800), granzyme B (Bioss, 1:100), IFN‐β (Bioss, 1:100) and glypican‐3 (Abcam, 1:200) at 4°C overnight. After being washed, the sections were reacted with HRP‐conjugated second antibodies and visualized with DAB. IHC signals were photoimaged (200 × magnification) under a light microscope (BX43 type, OLYMPUS). In addition, the frequency of apoptotic cells in tumor sections was measured by TUNEL assay using the TUNEL Detection Kit (ROCHE), according to the manufacture instructions.

Zhao J., Qin L., He G., Xie T., Hu G., Wang F., Zhong H., Zhu J, & Xu Y. (2023). Administration of a glypican‐3 peptide increases the infiltration and cytotoxicity of CD8 + T cells against testicular yolk sac tumor, associated with enhancing the intratumoral cGAS/STING signaling. Cancer Medicine, 12(23), 21293-21307.

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