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Anti cd90.2 microbeads

Manufactured by Miltenyi Biotec
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Anti-CD90.2 microbeads are a laboratory product designed for cell separation and purification. They consist of magnetic beads coated with antibodies specific to the CD90.2 cell surface antigen. These microbeads can be used to isolate and enrich target cell populations from complex samples, such as tissue or blood, through magnetic separation techniques.

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Lab products found in correlation

Pan t cell isolation kit 2, by Miltenyi Biotec (3 mentions) Mouse pan t cell isolation kit 2, by Miltenyi Biotec (3 mentions) H 2kd, by Thermo Fisher Scientific (2 mentions) Regulatory t cell isolation kit, by Miltenyi Biotec (2 mentions) Pan t isolation kit, by Miltenyi Biotec (2 mentions) Anti mouse cd3, by Thermo Fisher Scientific (2 mentions) Cd16 32, by Thermo Fisher Scientific (2 mentions) H 2kb, by Thermo Fisher Scientific (2 mentions) Automacs system, by Miltenyi Biotec (2 mentions) Mouse regulatory t cell isolation kit, by Miltenyi Biotec (2 mentions) Anti cd45 microbeads, by Miltenyi Biotec (2 mentions) Ls column, by Miltenyi Biotec (2 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) αgalcer, by Avanti Polar Lipids (1 mentions) Cytometric bead array technique, by BD (1 mentions) Isort automated cell sorter, by Thermo Fisher Scientific (1 mentions) Automacs separator, by Miltenyi Biotec (1 mentions) Anti ter119 microbeads, by Miltenyi Biotec (1 mentions) Anti pe microbeads ultrapure, by Miltenyi Biotec (1 mentions) Anti cd4 microbeads, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

CD90.2 microbeads (57 citations) CD90 microbeads (14 citations) Anti-CD90.2 (8 citations) Anti-CD90-MicroBeads (6 citations) Anti-CD90.2 and anti-CD45R(B220) microbeads (2 citations)

27 protocols using anti cd90.2 microbeads

1

Acute GVHD Induction Protocol

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Acute GVHD was induced as described previously.[20b] In brief, WT or Socs1 cKO donor mice were sacrificed the day after the last dose was given. Splenic T cells were isolated by negative selection using a Pan T Cell Isolation Kit II (Miltenyi‐Biotec, Germany), and the obtained cells had a purity of >95%. BM cells from WT mice were T cell‐depleted with anti‐CD90.2 MicroBeads (Miltenyi‐Biotec). BALB/c recipient mice received 8 Gy total body irradiation, and 3 × 106 or 2 × 106 T cells from the spleen of cKO mice or WT mice were transplanted intravenously the following day. 5 × 106 T cell‐depleted bone marrow cells (TCD‐BM) were transplanted as protective cells from the WT group donor mice to all groups of recipient mice.
Guo H., Li R., Wang M., Hou Y., Liu S., Peng T., Zhao X., Lu L., Han Y., Shao Y., Chang Y., Li C, & Huang X. (2022). Multiomics Analysis Identifies SOCS1 as Restraining T Cell Activation and Preventing Graft‐Versus‐Host Disease. Advanced Science, 9(21), 2200978.
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2

Isolation and Enrichment of T Cells from PBMCs

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Cells were isolated from heparinized blood using Ficoll-Paque PLUS (GE Healthcare) according to the manufacturer’s protocol. Briefly, blood was layered above Ficoll-Paque PLUS and centrifuged. The mononuclear cell layer (PBMCs) was centrifuged (200xg) twice to remove platelets. If PBMCs were from mice treated in vivo, RNA was immediately extracted. If PBMCs from naïve mice were to be treated ex vivo, they were separated into CD90+ T cells and CD90− PBMC−Ts. T cell enrichment: PBMCs were incubated with anti-CD90.2 microbeads (Miltenyi). Target cells were separated in a magnetic field with positive (retained-eluted T cells) and negative (fall-through PBMC−Ts) fractions collected. Cd3d expression (T cell marker (Haeryfar and Hoskin, 2004 (link))) was ~700-fold higher in T cells than PBMC−Ts; indicating efficient T cell enrichment and removal from the PBMC−T fraction. Cells were rested for 1.5h, treated for 6h in serum-free medium then stored at −80°C for later extraction.
Brooks A.K., Janda T.M., Lawson M.A., Rytych J.L., Smith R.A., Ocampo-Solis C, & McCusker R.H. (2017). Desipramine decreases expression of human and murine indoleamine-2,3-dioxygenases. Brain, behavior, and immunity, 62, 219-229.
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3

Isolation of T Cell Subsets from Murine Bone Marrow and Spleen

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Donor BM cells were isolated from the femurs of B6 WT mice. TCD-BM was prepared using anti-CD90.2 microbeads (purity >92%) (Miltenyi). The percentage of T cells in TCD-BM after sorting was less than 2% (Figure S6). Donor T cells were purified from the spleens of B6 WT or Gal-3−/− mice by negative selection using mouse pan T cell isolation kit II (Miltenyi) (purity >93%) (Figure S6). CD4+ T cells were purified using a pan T cell isolation kit II plus biotinylated anti-CD8 antibody or CD 8+ biotin plus CD25+ biotin antibodies, respectively (purity >90%). CD8+ T cells were isolated using the CD8+ T cell isolation kit.
Mohammadpour H., Tsuji T., MacDonald C.R., Sarow J.L., Rosenheck H., Daneshmandi S., Choi J.E., Qiu J., Matsuzaki J., Witkiewicz A.K., Attwood K., Blazar B.R., Odunsi K., Repasky E.A, & McCarthy P.L. (2023). Galectin-3 expression in donor T cells reduces GvHD severity and lethality after allogeneic hematopoietic cell transplantation. Cell reports, 42(3), 112250.
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4

Allogenic Bone Marrow Transplant in Mice

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All donor BM cells were isolated from C57BL/6 (H-2b) mice. T cells depletion of bone marrow was performed with auto-MACS by using anti-CD90.2 microbeads (Miltenyi Biotec). Donor CD4+ T cells (purity ∼95%) were purified from the spleens of C57BL/6 or GM-CSF-/- mice (H-2b) by using the CD4 beads (Miltenyi Biotec). The BALB/c (H-2d) hosts were irradiated with 9 Gy from [137Cs] source. One day later, the hosts were injected i.v. with 2 × 106 BM cells only or combined with 0.3 × 106 CD4+ T cells isolated from C57BL/6 or GM-CSF KO mice. The hosts were weighed every two days and monitored for survival. The H-2Kb+ myeloid cells were gated and analyzed for CD11b+Gr1+ cells by FACS in spleen and bone marrow in host at 11 days and 14 days after donor bone marrow cells transfer.
Paschall A.V., Zhang R., Qi C.F., Bardhan K., Peng L., Lu G., Yang J., Merad M., McGaha T., Zhou G., Mellor A., Abrams S.I., Morse HC I.I.I., Ozato K., Xiong H, & Liu K. (2015). IFN Regulatory Factor 8 Represses GM-CSF Expression in T cells to Affect Myeloid Cell Lineage Differentiation. Journal of immunology (Baltimore, Md. : 1950), 194(5), 2369-2379.
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5

Regulatory T Cell Isolation Protocol

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Pan T isolation kits, regulatory T cell isolation kits, and anti-CD90.2 microbeads were purchased from Miltenyi Biotec. Antibodies including anti-mouse CD3, TCRβ, CD4, CD8, CD25, H-2Kb, H-2Kd, and CD16/32 were purchased from eBioscience.
Du W., Leigh N.D., Bian G., Alqassim E., O'Neill R.E., Mei L., Qiu J., Liu H., McCarthy P.L, & Cao X. (2016). Granzyme B Contributes to the Optimal Graft-Versus-Tumor Effect Mediated by Conventional CD4+ T Cells. Journal of immunology research and therapy, 1(1), 22-28.
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6

Isolation and Activation of iNKT Cells in Mice

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Double-positive thymocytes were isolated from WT, VavCreGCSf/f, and CD1d–/– thymi after depleting cells reactive with PBS57-loaded CD1d tetramers. 0.5 × 106 DP thymocytes per well were placed in 96-well-plate and incubated with αGalCer (Avanti Polar Lipids, Alabaster, AL, USA) at indicated concentrations. iNKT cells were enriched from livers of TCRVα14-Jα281 transgenic mice using anti-CD5 micro beads (Miltenyi Biotec) and applied at 50,000/well. Activation of T cells in vitro was performed as described in Ref. (60 (link)). Briefly, splenic T-cells were enriched by anti-CD90.2 micro beads (Miltenyi Biotec) and incubated with 0.5 mg/ml calcium ionophore A23187 and 10 ng/mL phorbol 12-myristate 13-acetate (PMA, both Sigma). Supernatants were collected after 18 h and analyzed for IFNγ and IL4 concentrations by cytometric bead array technique (BD). For the in vivo testing of iNKT cells function, mice were injected i.p. with 0.2 or 3 µg αGalCer and sacrificed 8 h later.
Popovic Z.V., Rabionet M., Jennemann R., Krunic D., Sandhoff R., Gröne H.J, & Porubsky S. (2017). Glucosylceramide Synthase Is Involved in Development of Invariant Natural Killer T Cells. Frontiers in Immunology, 8, 848.
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7

Isolation of T, Ter119+, and CD71+ Cells

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T cells were purified from spleens by magnetic bead separation using anti-CD90.2 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) on an autoMACS separator (Miltenyi Biotec) according to the manufacturer's instructions. Similarly, Ter119+ cells were purified using the same system and anti-Ter119 microbeads (Miltenyi Biotec). CD71+ cells were isolated by jet-in-air cell sorting using an iSort Automated Cell Sorter (ThermoFischer) after staining with AF488-labeled anti-CD71 (clone RI7217, BioLegend).
Beckmann N., Huber F., Hanschen M., St. Pierre Schneider B., Nomellini V, & Caldwell C.C. (2020). Scald Injury-Induced T Cell Dysfunction Can Be Mitigated by Gr1+ Cell Depletion and Blockage of CD47/CD172a Signaling. Frontiers in Immunology, 11, 876.
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8

Allogeneic Bone Marrow Transplant in Mice

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On day 0, female BDF1 mice were conditioned by lethal irradiation divided into 2 doses (5 Gy each), 6 hours apart. Recipient mice were injected with 5 × 106 splenocytes from Ly 5.1 B6 mice and 5 × 106 TCD-BM cells from B6 mice on day 0 (allogeneic group). T cell depletion from donor bone marrow cells was conducted using anti-CD90.2 MicroBeads and an AutoMACS system (Miltenyi Biotec), according to the manufacturer’s instructions. The syngeneic group was administered the same amount of splenocytes and TCD-BM cells from the BDF1 mice.
Sumii Y., Kondo T., Ikegawa S., Fukumi T., Iwamoto M., Nishimura M.F., Sugiura H., Sando Y., Nakamura M., Meguri Y., Matsushita T., Tanimine N., Kimura M., Asada N., Ennishi D., Maeda Y, & Matsuoka K.I. (2023). Hematopoietic stem cell–derived Tregs are essential for maintaining favorable B cell lymphopoiesis following posttransplant cyclophosphamide. JCI Insight, 8(8), e162180.
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9

Purification of T Cell Subsets from Mouse Spleens

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The donor mice were in the C57BL/6J strain or the 129/SvJ strain (both H-2b). All donor bone marrow (BM) cells were isolated from WT mice. T cell depletion (TCD) was performed with auto-MACS by using anti-CD90.2 microbeads (Miltenyi Biotec). Donor CD4+CD25− T cells (purity > 90%) were purified from the spleens by using Pan T isolation kit II combined with biotin-conjugated anti-CD8 and anti-CD25 antibodies. Donor CD4+CD25+ regulatory T cells (purity > 90%) were purified from the spleens by using mouse regulatory T cell isolation kit (Miltenyi Biotec). Donor CD8+ T cells (purity > 90%) were purified from the spleens by using Pan T isolation kit II combined with biotin-conjugated anti-CD4 antibody. Donor total T cells (purity > 90%) were purified from the spleens by using Pan T isolation kit II.
Du W., Leigh N.D., Bian G., Alqassim E., O'Neill R.E., Mei L., Qiu J., Liu H., McCarthy P.L, & Cao X. (2016). Granzyme B Contributes to the Optimal Graft-Versus-Tumor Effect Mediated by Conventional CD4+ T Cells. Journal of immunology research and therapy, 1(1), 22-28.
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10

Regulatory T Cell Isolation Protocol

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Pan T isolation kits, regulatory T cell isolation kits, and anti-CD90.2 microbeads were purchased from Miltenyi Biotec. Antibodies including anti-mouse CD3, TCRβ, CD4, CD8, CD25, H-2Kb, H-2Kd, and CD16/32 were purchased from eBioscience.
Du W., Leigh N.D., Bian G., Alqassim E., O'Neill R.E., Mei L., Qiu J., Liu H., McCarthy P.L, & Cao X. (2016). Granzyme B Contributes to the Optimal Graft-Versus-Tumor Effect Mediated by Conventional CD4+ T Cells. Journal of immunology research and therapy, 1(1), 22-28.
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