Detailed information regarding the high-resolution mass spectrometry analysis is provided in the Supporting Information (Experimental Section ). Briefly, liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS; BRUKER impact II) was used to acquire the mass spectra. And a ZORBAX 300 SB-C18 column (0.5 × 150 mm, 5 μm, Agilent) and Luna NH2 100 Å column (1 × 150 mm, 3 μm, Phenomenex) were used in the separation of metabolites in reverse phase (positive mode) and HILIC analysis (negative mode), respectively.
Luna nh2 100 column
Manufactured by Phenomenex
Sourced in Germany
The Luna NH2 100 Å column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of polar compounds. The column features a spherical silica-based stationary phase with an aminopropyl (NH2) functional group that provides a hydrophilic interaction for the retention of polar analytes. The 100 Å pore size of the column allows for the effective separation of small to medium-sized molecules. This column is suitable for a variety of applications, including the analysis of carbohydrates, amino acids, and other polar compounds.
Automatically generated - may contain errors
4 protocols using luna nh2 100 column
1
High-Resolution Mass Spectrometry Metabolite Analysis
2
HPLC-RID Analysis of Sugars
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Waters high-performance liquid chromatography (HPLC) system (Milford, CT, USA) and a Waters 2414 refractive index detector (RID) were employed for chromatographic analysis of fructose, glucose, and sucrose. The separation of sugar compounds was performed on a Luna® NH2 100 Å column (250 × 4.6 mm; 5 µm particle size) purchased from Phenomenex (Torrance, CA, USA). For the chromatographic analysis of sugars, the following conditions were used. The mobile phase consisted of acetonitrile and water (80:20, v/v), which were degassed before use; the injection volume was 10.0 µL; a flow rate of 1.3 mL/min was used; and a run time of 15 min per sample was maintained. The retention times of glucose, fructose, and sucrose were 5.368 min, 6.255 min, and 8.057 min, respectively. The method used for the HPLC-RID analysis was validated according to international guidelines. The preparation procedure was carried out by measuring 5 g of sample and adding water and methanol (3:1, v/v). After filtering, the sample was introduced into the HPLC-RID autosampler vial. Each sample was assessed in triplicate.
3
Semi-preparative HPLC Purification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The semi-preparative purifications were performed with a commercially HPLC system composed by a Hitachi LaChrom L-7100 pump available from Merck equipped with a Sedex 75 evaporative light scattering detector (ELSD) available from Alfatech. The chromatographic separation of products was performed with a Luna NH2 100 Å column (250 × 10 mm, particle size 5 μm, Phenomenex, Aschaffenburg, Germany) at room temperature. The flow rate was set to 4.7 mL/min. The temperature of detection was set to 52 °C. EZ Chrome Elite software by Agilent was used for data management.
4
HPLC Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The analytical data were collected with a HPLC system composed by a Hitachi LaChrom L-7100 pump available from Merck (DE) equipped with a Sedex 75 evaporative light scattering detector (ELSD) available from Alfatech (FR). The chromatographic separation of products was performed with a Luna NH2 100 Å column (250 × 4.6 mm, particle size 5 μm, Phenomenex, Aschaffenburg, Germany) equipped with the corresponding guard column (4 × 3.0 mm), and kept at 30 °C with a Merck T-6300 column thermostat. The mobile phase was acetonitrile/water (80:20 v/v) at a flow rate of 1.0 mL/min. The temperature of detection was set to 52 °C. EZ Chrome Elite software by Agilent was used for data management.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!