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Dsp lomants reagent

Manufactured by Thermo Fisher Scientific
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DSP-Lomants reagent is a laboratory chemical used in the analysis and quantification of certain compounds. It functions as a colorimetric reagent, allowing for the detection and measurement of specific analytes through color change reactions. The core purpose of this product is to facilitate analytical procedures in research and diagnostic settings, but a detailed description cannot be provided while maintaining an unbiased and strictly factual approach.

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Lab products found in correlation

Vivaspin centrifugal concentrator, by Sartorius (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions) Gfp trap beads, by Merck Group (1 mentions)

2 protocols using dsp lomants reagent

1

Purified Mutant RVFV N Protein Analysis

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The purified mutant RVFV N proteins were concentrated to 0.2 μg/μl in 25 μl PBS/glycerol using Vivaspin centrifugal concentrators (Sartorius). Dithiobis(succinimidyl propionate)(DSP)-Lomants reagent (Thermo Scientific) was added to a final concentration of 1 mM and gently mixed, then incubated at 20°C for 40 min. The reaction was stopped by directly adding protein loading buffer under non-reducing conditions and then analysed on a 4–12% Bis-Tris plus (Novex) gradient gel.
Mottram T.J., Li P., Dietrich I., Shi X., Brennan B., Varjak M, & Kohl A. (2017). Mutational analysis of Rift Valley fever phlebovirus nucleocapsid protein indicates novel conserved, functional amino acids. PLoS Neglected Tropical Diseases, 11(12), e0006155.
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2

Endogenous Protein Immunoprecipitation

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Endogenous immunoprecipitation was performed using DSP (Lomant’s Reagent) (Thermo Scientific) on intact cells at the indicated concentrations for 30 min on ice. Tagged proteins were immunoprecipitated with either GFP-Trap beads or anti-FLAG M2 affinity gel (Sigma). See Supplemental Experimental Procedures for more information.
Dooley H.C., Razi M., Polson H.E., Girardin S.E., Wilson M.I, & Tooze S.A. (2014). WIPI2 Links LC3 Conjugation with PI3P, Autophagosome Formation, and Pathogen Clearance by Recruiting Atg12–5-16L1. Molecular Cell, 55(2), 238-252.
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