The serum testosterone was measured by using the commercially available testosterone ELISA kit (Biocheck, USA). About 20 μl of calibrator and serum was added into appropriate wells of strips. About 200 μl of horseradish peroxidase testosterone conjugate was added. The mixture was incubated for 2 h at 37oC. The wells were rinsed and immediately 100 μl of chromogen substrate mixture and 0.01% hydroperoxidase in citrate buffer was added. Absorbance was immediately read at 450 nm in ELISA reader (Arfat et al., 2014 ).
Testosterone elisa kit
Manufactured by Biocheck
Sourced in United States
The Testosterone ELISA kit is a laboratory equipment used for the quantitative determination of testosterone levels in human serum or plasma samples. It employs the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of testosterone.
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Lab products found in correlation
3 protocols using testosterone elisa kit
1
Serum Testosterone Quantification by ELISA
2
Testosterone and DHT Measurement Protocol
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Serum testosterone and DHT levels were determined by using testosterone ELISA kit (Biocheck, USA) and DHT ELISA kit (Abnova, USA) following the manufacturer’s instructions.
3
Serum Testosterone and PSA Measurement
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After the 5 weeks of the experiment, the rats were fasted overnight and then overdosed with Ketamine and Xylazine before they were sacrificed. To obtain the serum, we took blood samples directly from the abdominal aorta and centrifuged them for 15 minutes at 3000 g. A testosterone ELISA kit (Biocheck, USA) and DHT ELISA kit (Abnova, USA) were used to measure TE and prostate specific antigen (PSA) in serum using an enzyme-linked immunosorbent assay (ELISA) [34, (link)46] (link).
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