Darunavir

We purchased econazole nitrate, duloxetine hydrochloride, doxapram hydrochloride monohydrate, clemastine fumarate salt, sertaconazole nitrate, isavuconazole, rupintrivir, and zopiclone from Sigma-Aldrich; pimavanserin, trihexyphenidyl hydrochloride, reboxetine mesylate, sertindole, bepridil hydrochloride, darunavir, nelfinavir mesylate, indinavir sulfate, lopinavir, tipranavir, saquinavir, pirenzepine hydrochloride, oxiconazole nitrate, pimozide, and rimonabant from Cayman Chemicals; ebastine and itraconazole from Alfa Aesar; metixene hydrochloride hydrate and lemborexant from MedChemExpress; fexofenadine hydrochloride from TCI Chemicals; ketoconazole from Acros Organics; clotiapine from Enzo Life Sciences; and oxyphencyclimine from Boc Sciences. We acquired Sub3 with the sequence as DABCYL−Lys−Thr−Ser−Ala−Val−Leu−Gln−Ser−Gly−Phe−Arg−Lys−Met−Glu−EDANS from Bachem Inc. DABCYL is the abbreviated form of 4-(dimethylaminoazo)benzene-4-carboxylic acid and EDANS is the abbreviated form of 5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid.

Molecular biology grade DMSO was purchased from Sigma Aldrich (St. Louis, MO, USA), Sterile PBS was from ThermoFisher Scientific (Waltham, MA, USA). 3CLpro inhibitor screening enzymatic assay kits (Catalog #79955-1) were from BPS Biosciences (San Diego, CA, USA). Amprenavir, Atazanavir sulfate, Candicin, Chloroquine Phosphate, Hydroychloroquine Sulfate, and Lopinavir were purchased from Sigma Aldrich (Saint Louis, MO). Beclabuvir, Temsavir were from Medchem Express (Monmouth Junction, NJ). Abacavir (sulfate), Arbidol hydrochloride, Asunaprevir, Atrovastatin, Boceprevir, Daclatasvir, Danoprevir, Darunavir, Delavirdine (mesylate), Edoxudine, Elbasvir, Elvitegravir, Etravirine, Favipiravir, Fumagillin, Glecaprevir, Grazoprevir, Indinavir sulfate, Itraconazole, Ivermectin, Ledipasvir (G-5885), Maraviroc, Methylprednisolone, micafungin sodium, ombitasvir, Oseltamivir phosphate, Paritaprevir, Peramivir, Pibrentasvir, Pimodivir, Pleconaril, Posaconazole, Quinine, Raltegravir (potassium salt), Remdesivir, Ribavirin 5’-monophosphate (lithium salt), Ribostamycin sulfate, Rilpivirine, Saquinavir, Telaprevir, Tenofovir diphosphate (sodium salt), and Velpatasvir were purchased from Cayman Chemicals (Ann Arbor, MI).