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Rnaimax

Manufactured by InvivoGen
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RNAimax is a transfection reagent designed to facilitate the delivery of small interfering RNA (siRNA) and short hairpin RNA (shRNA) into a variety of cell lines. It is optimized to enable efficient gene silencing by RNA interference (RNAi).

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Lab products found in correlation

Close spelling variants of this product

Lipofectamine RNAiMAX (3 citations)

2 protocols using rnaimax

1

Silencing ASFV DP96R Gene Using siRNA

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The specific small interfering RNA (siRNA) for targeting ASFV DP96R was designed and synthesized by Bioneer (Daejeon, Republic of Korea). Primary PAMs (cell number 1 × 106 in 24-well plates) were transfected with negative control (siControl) or DP96R (siDP96R) siRNA by RNAimax (Invivogen). The target sequence of DP96R was 256- GGAUCCCUAAUGCGCUCCA-274. Nontargeting siRNA was used as a negative control (UUCUCCGAACGUGUCACGU). At 6 hpt, the cells were left uninfected or infected with ASFV at a multiplicity of infection (MOI) of 0.5. The mRNA expression levels of the target gene, IFNs, and ISG mRNA were detected by qRT-PCR. All experiments dealing with ASFV were conducted in accordance with the Standard Operating Procedure (SOP) in the biosafety level 3 (BSL-3) laboratory of the NIWDC in Republic of Korea.
Dodantenna N., Cha J.W., Chathuranga K., Chathuranga W.A., Weerawardhana A., Ranathunga L., Kim Y., Jheong W, & Lee J.S. (2024). The African Swine Fever Virus Virulence Determinant DP96R Suppresses Type I IFN Production Targeting IRF3. International Journal of Molecular Sciences, 25(4), 2099.
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2

Silencing RIG-I and MDA5 via siRNA

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Knockdown of RIG-I and MDA5 was achieved using Silencer® Select Pre-designed siRNA and RNAiMAX (Invivogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. The sense sequences for RIG-I #1 and #2 were 5'-GAAGCAGUAUUUAGGGAAATT-3' (antisense: 5'-UUUCCCUAAAUACUGCUUCGT-3') and 5'-CCAGAAUUAUCCCAACCGATT-3' (antisense: 5'-UCGGUUGGGAUAAUUCUGGTT-3'), respectively. The sense sequences for MDA5 #1 and #2 were 5'-GUAACAUUGUUAUCCGUUATT-3' (antisense: 5'-UAACGGAUAACAAUGUUACAT-3') and 5'-GGUGUAAGAGAGCUACUAATT-3' (antisense: 5'-UUAGUAGCUCUCUUACACCTG-3'), respectively. Silencer® Select Negative Control #1 (sequence not available) was used as the negative control. The final concentration of all siRNAs was 10 nM. After 48 h transfection at 37˚C in a humidified atmosphere of 5% CO2/95% air, cells were harvested. The cells were immediately seeded onto collagen I-coated dishes for subsequent experiments.
Sasaki F., Yoshino H., Kusuhara A., Sato K, & Tsuruga E. (2024). Involvement of retinoic acid‑inducible gene‑I in radiation‑induced senescence of human umbilical vein endothelial cells. Biomedical Reports, 20(4), 70.
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