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Rcm monosaccharide ca2 column

Manufactured by Phenomenex
Sourced in United States

The RCM-Monosaccharide Ca2+ column is a chromatographic column designed for the analysis of monosaccharides. It utilizes a calcium-based stationary phase to facilitate the separation of various monosaccharide compounds.

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2 protocols using rcm monosaccharide ca2 column

1

HPLC Quantification of Glucose and Glucosone

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An Agilent 1200 series HPLC instrument equipped with a Phenomenex Rezex (Torrance, CA) RCM-Monosaccharide Ca2+ column at 85 °C, a water mobile phase (0.3 ml/min), and a refractive index detector (RID) was used to quantify glucose and glucosone. An equivalent response factor for glucose and glucosone was used for quantitation [50 (link)].
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2

Extraction and Analysis of Organic Acids and Sugars in Rosehips

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The extraction of organic acids and sugars was performed and analyzed according to the method described by Mikulic-Petkovsek et al. [55 (link)]. Frozen rosehips (0.5 g), from which the seeds had been previously removed, were finely crushed, and 1.5 mL of bidistilled water was added. The samples in plastic centrifuge tubes were placed on a shaker (Unimax 1010, Heidolph Instruments, Hamburg, Germany) for half an hour. The extracts were centrifuged at 10,000× g for 7 min at 4 °C (Eppendorf Centrifuge 5810 R, Hamburg, Germany). The supernatant was filtered into a labeled vial through a syringe filter (Chromafil Xtra MV-20/25, Macherey Nagel, Dueren, Germany). Until further analysis, the samples were stored in a freezer at −20 °C. The extraction was performed in triplicate. We used a UV detector set to 210 nm for separating organic acids, a Rezex ROA column (Phenomenex, Torrance, CA, USA) heated to 65 °C, and a mobile phase containing 4 mM sulfuric acid at a flow rate of 0.6 mL/min. Sugars were also separated at 65 °C on a Rezex (Phenomenex, Torrance, CA, USA) RCM monosaccharide Ca + (2%) column (300 mm × 7.8 mm). The flow rate was 0.6 mL/min continuously, 30 min total run time, and bidistilled water as the mobile phase. Carbohydrates were detected using a refractive index (RI) detector.
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