Bis acrylamide

PC12 cells were obtained from Pasteur Institute (Tehran, Iran), DMEM (Gibco, Grand Island, NY,USA), p-Akt1/2/3 Antibody (Ser 473): sc-7985 (SANTA CRUZ, CA, USA), p-p38 Antibody (Tyr 182): sc-101759 (SANTA CRUZ, CA, USA), p38α Antibody (N-20): sc-728 (SANTA CRUZ, CA, USA), p-JNK Antibody (14.Thr 183/Tyr 185): sc-293136 (SANTA CRUZ, CA, USA), JNK1/3 Antibody (C-17): sc-474, RIPA Buffer (Sigma-Aldrich, New York, NY, USA), Anti-Protease Cocktail (Sigma-Aldrich, New York, NY, USA), Acrylamide and bisAcrylamide (Sigma-Aldrich, New York, NY, USA).

The mung bean seeds were obtained from Sri Venkateswara Agricultural University, Tirupati. Soybean LOX, ETYA, Linoleic acid, Linolenic acid, Arachidonic acid, Sephadex G-100, DE-52, Poly Buffer Exchangers 94 (PBE-94), Phenyl methyl sulfonyl fluoride (PMSF), EDTA, Acrylamide, Bis-Acrylamide, Coomassie brilliant blue, Lauryl sulphate (SDS) and protein size markers were procured from Sigma Chemicals Co (St. Louis, MO, USA). NDGA was a generous gift from department of chemistry, S.V. University. All other chemicals were reagent grade procured from Merck, Mumbai, India.

Jatropha curcas seed cake was obtained from Instituto Fazenda Tamanduá (Paraíba, Brazil), grounded in a coffee grinder and passed through a 1-mm-mesh screen. The resulting flour was treated with n-hexane (1:5, m/v) to remove the remaining oil left after biodiesel extraction. Defatted flour was stored in air-tight containers at 4°C until analysis. The bacteria Salmonella enterica subspecies enterica serovar choleraesuis (ATCC 10708), Bacillus subtilis subspecies spizizenii (ATCC 6633), Pseudomonas aeruginosa (ATCC 25619), and Staphylococcus aureus (ATCC 25923) were obtained from the Department of Biology (UFC), Fortaleza, Brazil. Swiss mice (Mus musculus), 20–25 g, were from the animal house at UFC. Azocasein, bovine pancreatic trypsin, bovine pancreatic chymotrypsin, bovine serum albumin (BSA), soybean trypsin inhibitor (SBTI), Nα-benzoyl-D,L-arginina-p-naftilamida (BANA), 4-(dimethylamino)cinnamaldehyde (DMACA), ethylenediaminetetraacetic acid (EDTA), phenylmethylsulfonyl fluoride (PMSF), L-cysteine and Nα-benzoyl-D,L-arginine 4-nitroanilide hydrochloride (BAPNA), sodium dodecyl sulfate (SDS), molar mass markers, acrylamide, bis-acrylamide, dithiothreitol (DTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chromatographic matrixes were from GE Healthcare. All other chemicals and reagents used were of analytical grade.

Components for culture media, tryptone, and yeast extract were purchased from Difco Laboratories, India. Glycine, phenylmethylsulfonyl fluoride (PMSF), isopropyl β-D-1-thiogalactopyranoside (IPTG), Tris buffer and sodium dodecyl sulfate (SDS) were from Amresco, United States. Glucose and NaCl were purchased from Qualigen, India. Low molecular weight marker for SDS-PAGE was from GE Healthcare, United States. Dithiothreitol (DTT), acrylamide, bis-acrylamide, urea, ammonium persulfate (APS) and Proteinase K were purchased from Sigma-Aldrich, United States. Bromophenol blue, Tetramethylethylenediamine (TEMED), and Ethylenediaminetetraacetic acid (EDTA) were procured from BIO-RAD, United States.

Chemicals required to synthesise photolabile monomer included 4,5-dimethoxy-2-nitrobenzyl chloroformate (NVOC), methacrylamide poly(ethylene glycol) amine hydrochloride (M_n: 400), triethylamine (TEA), anhydrous tetrahydrofuran (THF), ethyl acetate, hydrochloric acid (HCl), and sodium sulphate (Na₂SO₄). These chemicals were bought from Sigma-Aldrich.
Ethanol, chloro(dimethyl)vinyl silane (CDVS), toluene, 40% (w/v) solution of 29 : 1 (w/w) acrylamide : bis-acrylamide, ammonium persulphate (APS), N,N,N′,N′-tetramethylethylenediamine (TEMED), sodium phosphate monobasic monohydrate, sodium phosphate dibasic dodecahydrate, sodium phosphate tribasic dodecahydrate, 1 M sodium hydroxide, hydroxylamine (NH₂OH) hydrochloride, and fluorescein isothiocyanate (FITC) were bought from Sigma-Aldrich. Microscope glass slides were bought from VWR. These materials and chemicals were used to fabricate photofunctionalisable hydrogel films on glass substrates.
Materials used to make fluidic flow cells consisted of 3 mm thick transparent poly(methyl methacrylate) (PMMA) sheets, bootlace ferrules (211-4252), and nitrile O-rings (01-08-01801), and were purchased from Fred Aldous, RS Components, and Ashton Seals, respectively.

Low molecular weight chitosan (50-190 kDa, 75-85% deacetylated) (SC), sildenafil citrate (SF), 5,5′-dithiobis nitrobenzoic acid (DTNB), nicotinamide adenine dinucleotide phosphate (NADPH), bovine serum albumin (BSA), reduced glutathione (GSH), 1-chloro-2,4-dinitrobenzene (CDNB), epinephrine, acrylamide, bisacrylamide, tetramethylethylenediamine (TEMED), cumene hydroperoxide, and tris-HCl were purchased from Sigma Aldrich, Germany. Sodium tripolyphosphate (TPP) was purchased from Loba Chemie, India. Folin-Cioclateu phenol reagent was purchased from Oxford Lab Chem, India. Tween 80, potassium phosphate, trichloroacetic acid (TCA), thiobarbituric acid (TBA), sodium phosphate, hydrogen peroxide (H₂O₂), sodium carbonate, magnesium chloride, acetone, sodium hydroxide (NaOH), sodium borate, sodium carbonate (Na₂CO₃), copper sulfate (CuSO₄), Na-K tartrate, sulphosalicylic acid, ammonium persulphate (APS), and sodium dodecyl sulfate (SDS) were purchased from El-Nasr Pharmaceutical Company, Egypt. Primary anti-mouse antibodies for GPx and GST were obtained from Santa Cruz Co., USA.