When comparing mass cytometry and flow cytometry data, viability staining was also performed with Fixable Violet Dead Cell Stain (Thermo Fisher Scientific #L34955). Samples were then fixed as described above and separated into two parts for both flow and mass cytometry analysis just prior to storage at −80ºC. Surface staining was performed with anti-CD3ε-APC (clone UCHT1, Biolegend #300412) and anti-CD8α-APC-H7 (clone SK1, BD Biosciences #560273) antibodies. Cells were then resuspended in 250 µL CSM and analyzed on LSRII flow cytometer (BD Biosciences).
Fixable violet dead cell stain
Manufactured by Thermo Fisher Scientific
The Fixable Violet Dead Cell Stain is a laboratory reagent used to identify and distinguish between live and dead cells in a sample. It is designed to bind to dead cells, allowing for their detection and quantification.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fixable violet dead cell stain
1
Viability Staining for Cytometry
2
Viability Staining for Cytometry
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