Human (#407306) and mouse (#407320) IFN-γ, human IL-6 (#407652) were purchased from Merck Millipore, human IFN-β (#11415–1) was from PBL Assay Science. Human IL-6Rα was from R&D Systems (#227-58-025), human IL-27 from Peprotech (#200–38). Other treatments used in this work include LPS (#L7895, Sigma) and ratjadone (#553590, Merck Millipore). Unless stated otherwise, IFN treatment was for one hour with 50 U/ml IFN-γ or 500 U/ml IFN-β in growth medium. Similarly, unless stated otherwise, IL-27 treatment was performed for 40 min at a concentration of 100 ng/ml. IL-6/IL-6R cotreatments also lasted for 40 min at concentrations of 200 and 250 ng/ml, respectively.
Human il 6rα
Manufactured by R&D Systems
The Human IL-6Rα is a recombinant protein that corresponds to the extracellular domain of the human interleukin-6 receptor alpha subunit. It functions as a receptor component involved in the signaling of the cytokine interleukin-6.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using human il 6rα
1
Cytokine signaling in immune cells
2
Breast Cancer Cytokine Stimulation Assay
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