Cuy21
The CUY21 is a laboratory equipment designed for general scientific applications. It serves as a versatile tool for various research and testing purposes. The core function of the CUY21 is to provide a controlled environment for experiments and analyses, but a detailed description of its intended use cannot be provided while maintaining an unbiased and factual approach.
Lab products found in correlation
27 protocols using cuy21
In Utero Electroporation of Mouse Cortex
ChR2-venus Expression in Visual Cortex
ChR2-venus Expression in Visual Cortex
Animals were maintained with a light-dark cycle of 12:12 h, and up to four mice were kept in one cage after weaning.
In Utero CXCR5 Modulation in Mice
Multi-Lineage Tracing in Mouse Embryos
To achieve labeling of all progenitor descendants, the Tol-2 system was used57 (link). For this purpose, 1–2 μg/μl pCAGGS58 (link)-Tol2 transposase (T2TP) and 1 μg/μl pT2K-CAGGS-enhanced green fluorescent protein (EGFP)22 (link) were co-electorporated with 1 μg/μl pCAGGS-tandem dimer tomato (tdTomato) or monomeric cherry (mCherry)59 to wild type and 1–2 μg/μl pCAGGS-T2TP and 1 μg/μl pT2K-CAGGS-EGFP to Nkx2.1Cre; Ai9 mice. As a second method to label all progenitor descendants, 1 μg /μl pCAGGS-Cre and 1 μg/μl pCAGGS-EGFP60 (link) were co-electroporated to Ai9 mice embryos. In either method, plasmids were dissolved in PBS.
In all samples, GFP and tdTomato fluorescence were enhanced by immunostaining against GFP and DsRed, respectively.
In utero electroporation for gene knockdown
In Utero Electroporation of Murine Brain
Quantitative RT-PCR Analysis of Nmnat Knockdown
In utero electroporation for gene overexpression
In vivo Electroporation of Embryonic Mouse Brains
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