Monkey kidney MA104 and human intestinal Caco-2 cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA) and grown in alpha minimal essential medium (α-MEM) and Dulbecco’s modified Eagle’s medium (DMEM; Welgene, Daegu, South Korea), respectively, supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. Bovine RVA NCDV (G6P6[1]) and human RVA DS-1 (G2P1B[4]) strains were obtained from the ATCC and propagated in MA104 cells after preactivation with 10 μg/mL porcine trypsin (Gibco, Fort Worth, TX) as described previously (84 (link)). Viral titers were determined via immunofluorescence (IF) of infected cells using a monoclonal antibody (MAb) against the VP6 protein of RVA and were expressed as fluorescence focus units (FFU) per milliliter. In all experiments, a multiplicity of infection (MOI) of 1 was used.
Dulbecco s modified
Manufactured by Welgene
Sourced in United States
Dulbecco's Modified Eagle's Medium (DMEM) is a commonly used cell culture medium formulation that provides essential nutrients and growth factors required for the maintenance and growth of a wide variety of cell types. The medium is designed to support the optimal growth and proliferation of cells in vitro.
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Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Welgene (2 mentions)
Caco 2, by American Type Culture Collection (1 mentions)
Porcine trypsin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Genistein, by Merck Group (1 mentions)
Penicillin and streptomycin, by Welgene (1 mentions)
T24 cell line, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Ez cytox cell viability assay kit, by Daeil Lab Service (1 mentions)
Horse serum, by Merck Group (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Lsm 710, by Zeiss (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Alexa flour 488, by Thermo Fisher Scientific (1 mentions)
Confocal dishes, by SPL Life Sciences (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Dld 1 cells, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
1640 medium, by Welgene (1 mentions)
Colo320dm, by American Type Culture Collection (1 mentions)
7 protocols using dulbecco s modified
1
Propagation of Bovine and Human Rotaviruses
2
Cell Culture Conditions for Genistein Treatment
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The T24 cell line was purchased from the American Type Culture Collection (ATCC, Manassas, MD, USA) and grown in RPMI 1640 medium containing 10% heat-inactivated fetal bovine serum (FBS), and 1% penicillin and streptomycin (WelGENE Inc., Daegu, Korea). Normal non carcinoma cell lines, including myoblast C2C12 cells and lung fibroblast V79-4 cells were also obtained from the ATCC. C2C12 and V79-4 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, WelGENE Inc.) supplemented with 10% FBS, and 1% penicillin and streptomycin. All cell lines were grown at 37 °C in 5% CO2 humidified incubator. Genistein (Sigma-Aldrich Chemical Co., St. Louis, MO, USA) was dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich Chemical Co.) and then diluted to the appropriate concentration using culture medium before treatment to the cells.
3
Senescence Induction in Auditory Cells
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Low-dose H2O2 has been used to induce premature senescence in auditory cells34 (link),35 (link). HEI‐OC1 cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Welgene, Daegu, Korea) supplemented with 10% fetal bovine serum (FBS; Lonza Walkersville, MD, USA) at 33 °C under a humidified atmosphere with 5% CO2. The cells were treated with 2 mM H2O2 (Daejung Chemicals, Siheung, Korea) for 1 h to induce a senescent phenotype30 (link). Cell viability was detected using the EZ-Cytox cell viability assay kit (Daeil Lab Service, Seoul, Korea), according to the manufacturer’s protocol. The cells were pre-treated with 30 µM UA (Sigma, MO, USA) for 2 h, and then incubated with 2 mM H2O2 for 1 h. After washing with PBS, the cells were incubated in culture medium for 5 days.
4
Skeletal Muscle Cell Differentiation
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Mouse myoblast C2C12 skeletal muscle cells were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; WelGENE Inc., Korea) supplemented with 10% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco, Paisley, Scotland) contains 10,000 units/ml of penicillin, 10,000 μg/ml of streptomycin, and 25 μg/ml of Gibco Amphotericin B. The cells were maintained at 37°C in a humidified atmosphere containing 5%CO2. C2C12 myotubes were induced from C2C12 myoblasts using a differentiation medium (DM) composed of high-glucose DMEM supplemented with 2% horse serum (Sigma-Aldrich, USA) and 1% antibiotics. When the myoblast density reached 90% confluence, the growth medium was changed to DM. All differentiation experiments were performed for 6 days, and the DM was changed every 2 days.
5
Cellular Penetration Assessment via Immunofluorescence
6
Culturing Human CRC Cell Lines
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Human CRC cells (COLO320DM and DLD-1 cells) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were authenticated at Cosmogenetech (Seoul, Korea) based on the ATCC cellular information and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Welgene, Gyeongsangbuk-do, Korea) or Dulbecco’s modified Eagle’s medium (DMEM, Welgene) supplemented with 10% fetal bovine serum (FBS) (Welgene) and 100 units/mL penicillin–streptomycin (Gibco, Grand Island, NY, USA), followed by incubation at 37 °C in a 5% CO2 incubator.
7
Adipocyte Differentiation in 3T3-L1 Cells
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3T3−L1 preadipocytes were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Welgene, Daegu, Republic of Korea) supplemented with 10% bovine calf serum and 1% penicillin−streptomycin (Hyclone, Logan, UT, USA) at 37 °C in a humidified atmosphere of 5% carbon dioxide until confluence. To induce differentiation, two days after 100% confluence, the medium was replaced with a differentiation medium containing 10% fetal bovine serum (FBS; Hyclone), 0.5 mM isobutylmethylxanthine (Sigma−Aldrich, St. Louis, MO, USA), 1 μM dexamethasone (Sigma−Aldrich), and 10 μg/mL insulin (Sigma−Aldrich). After two days, the cells were cultured in DMEM supplemented with 10% FBS and 10 μg/mL insulin for an additional two days. Subsequently, the medium was replaced with DMEM containing 10% FBS every 48 h until day 8. Confluent pre−adipocytes were treated with rabbit meat extract and differentiation medium to induce differentiation for eight days.
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