Ethylenediamine, methyl acrylate, methanol, ethanol, toluene, pyridine, acetic anhydride, chloroform, hexanes, hydrochloric acid, magnesium sulfate, α-Cyano-4-hydroxycinnamic acid, Lugol’s iodine, glacial acetic acid, N-acetyl-D-penicillamine, and Ellman’s reagent were all purchased from Sigma Aldrich (St. Louis, MO, USA). ATTO-TAGTM FQ reagent was purchased from Invitrogen (Grand Island, NY, USA). Tert-butyl nitrite (90% technical grade, Acros Organics) was purchased from Fisher Scientific. Polyvinyl chloride (PVC) (MW 233,000, Mn = 99,000) was purchased from Sigma Aldrich (St. Louis, MO, USA).
Tert butyl nitrite
Manufactured by Thermo Fisher Scientific
Tert-butyl nitrite is a chemical compound used as a reagent in various laboratory applications. It is a colorless liquid with a characteristic odor. The core function of tert-butyl nitrite is to serve as a nitrosating agent, which is used in organic synthesis reactions.
Automatically generated - may contain errors
Lab products found in correlation
Ethylenediamine, by Merck Group (2 mentions)
Acetic anhydride, by Merck Group (2 mentions)
N acetyl d penicillamine, by Merck Group (2 mentions)
L penicillamine, by Merck Group (2 mentions)
Ethidium bromide, by Thermo Fisher Scientific (2 mentions)
Calcein am, by Merck Group (2 mentions)
Gelatin, by Bio-Rad (2 mentions)
Cyclam, by Merck Group (2 mentions)
Ellman s reagent, by Merck Group (1 mentions)
α cyano 4 hydroxycinnamic acid, by Merck Group (1 mentions)
Methyl acrylate, by Merck Group (1 mentions)
Polyvinyl chloride pvc, by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
Pyridine, by Merck Group (1 mentions)
4 protocols using tert butyl nitrite
1
Synthesis and Characterization of Peptide Derivatives
2
Fluorescent Labeling of Thiol-Containing Molecules
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Polyvinyl chloride (average Mw = 233,000, Mn = 99,000) (PVC), ethylenediamine, 5,5’-dithiobis (2-nitrobenzoic acid), triethylamine, 1,4,8,11-tetraazacyclotetradecane (cyclam), N-acetyl-d -penicillamine (Fluka), and concentrated hydrochloric acid were obtained from Sigma-Aldrich (St. Louis, MO, USA). ATTO-TAGTM FQ Amine-Derivatization Kit was purchased from Invitrogen (Grand Island, NY, USA). Tert-butyl nitrite (90% technical grade, Acros Organics) was purchased from Fisher Scientific. Magnesium sulfate (MgSO4), copper (II) bromide (CuBr2), L-ascorbic acid sodium salt, and acetic anhydride (Alfa Aesar) were purchased from VWR (West Chester, PA, USA).
3
Sylgard 184 Elastomer Characterization
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Silicone elastomer base & curing agent (Sylgard® 184) were ordered from Dow Corning Co. (Midland, MI). Penicillin-streptomycin (pen/strep), G418 disulfate, collagen I, N-acetyl-D,L-penicillamine, acetic anhydride, calcein-AM, hydrogen peroxide, di-tert-butyl peroxide, and cyclam were obtained from Sigma-Aldrich (St. Louis, MO). Tert-butyl nitrite was purchased from Acros Organics, (Pittsburgh, PA). Gelatin was obtained from Bio-rad (Hercules, CA). Pyridine was purchased from EMD Chemical Inc. (Darmstadt, Germany). Ethidium bromide, Click-iT® EdU assay kit and Hoechst dye were purchased from Invitrogen (Grand Island, NY). Smooth muscle cell line MOVAS, Dulbecco's modified eagle medium (DMEM), fetal bovine serum (FBS), were all purchased from ATCC (Manassas, VA).
4
Cellular and Material Characterization
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RAW264.7 cell, ARPE-19 cell, MOVAS cell, SVEC, and fetal bovine serum (FBS), phosphate buffered saline (PBS), Dulbecco's modified eagle medium (DMEM) were all purchased from ATCC (Manassas, VA). L-929 cell and mouse primary tenocyte (kindly provided by Dr. Rupak Rajachar, Houghton, MI). Silanol-terminated PDMS, (3-aminopropyl) trimethoxylsilane, dibutyltin dilaurate were purchased from Gelest Inc. (Morrisville, PA). Room temperature vulcanized PDMS (RTV-3140) and silicone elastomer base and curing agent (Sylgard® 184) were acquired from Dow Corning Co. (Midland, MI). Interferon-γ mouse was obtained from BD Biosciences (San Diego, CA), lipopolysaccharine, L-arginine, N-acetyl-D, L-penicillamine, acetyl anhydrate, calcein-AM, and 4-amino-5-methylamino-2′,7′-difluorescein (DAF-FM), and cyclam were obtained from Sigma-Aldrich (St. Louis, MO). Tertbutyl nitrite was purchased from Acros Organics, (Pittsburgh, PA). Toluene was purchased from Mallinckrodt Chemicals (Phillipsburg, NJ). Nω-hydroxy-nor-Arginine (nor-NOHA), L-NG-Nitroarginine methyl ester, and nitrate/nitrite colorimetric assay kit were obtained from Cayman Chemical (Ann Arbor, MI). Gelatin was obtained from Bio-rad (Hercules, CA). Pyridine was purchased from EMD Chemical Inc. (Darmstadt, Germany). Ethidium bromide was obtained from Invitrogen (Grand Island, NY).
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