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9 10 anthracenediyl bis methylene dimalonic acid

Manufactured by Merck Group
Sourced in United States

9,10-anthracenediyl-bis(methylene)dimalonic acid is a compound used as a reagent in laboratory settings. It serves as a core functional substance for various applications, without interpretation of its intended use.

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12 protocols using 9 10 anthracenediyl bis methylene dimalonic acid

1

Fluorescent Probes for Oxidative Stress

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Tetraethyl orthosilicate (TEOS), triethanolamine (TEAH), coumarin (C9H6O2, 99%), 9,10-anthracenediyl-bis(methylene) dimalonic acid (ABDA), sodium dithionite (SDT) and decahydronaphthalene (98%) were purchased from Sigma-Aldrich. Potassium permanganate (97%) was obtained from Shanghai Chemical Co. Dichloromethane (99.99%) was purchased from Aladdin Industrial Inc. Dodecacarbonyltriiron (Fe3(CO)12, 96%) was purchased from Macklin Industrial Inc. All chemicals were used as received without any further purification. Hemoglobin (Hb) of bovine red blood cells was purchased from MP Biomedicals. DAPI, Lyso-Tracker Green, 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) and 2-[6-(4,-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (HPF) were purchased from Beyotime Biotechnology.
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2

Synthesis and Characterization of PEG-DSPE Nanoparticles

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Dimethylsulfoxide (DMSO) and tetrahydrofuran (THF) were purchased from China National Medicines Co. Ltd. 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethylene glycol)-2000] (PEG-DSPE) was purchased from Xi’an Ruixi Biological Technology Co. Ltd. Phosphate buffered saline (PBS), fetal bovine serum (FBS), trypsin, Dulbecco’s Modified Eagle’s Medium (DMEM) and penicillin−streptomycin were purchased from Gibco Invitrogen Corporation. 9.10-Anthracenediyl-bis(methylene)dim alonic acid (ABDA) was provided by Sigma-Aldrich. 2.7-dichlorodihydrofluorescein diacetate (DCFH-DA), 5-chloromethyl fluorescein diacetate (CMFDA) and propidium iodide (PI) were provided by Yeasen Co., Ltd. HeLa cells were purchased from Procell Life Science&Technology Co., Ltd.
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3

Multifunctional Nanoparticle Drug Delivery System

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All materials and solvents were used as received without further purification. Dulbecco’s modified Eagle’s medium (DMEM) (high glucose with L-glutamine sterile-filtered) and fetal bovine serum (FBS) were purchased from Sigma-Aldrich. Hexadecyltrimethylammonium bromide (CTAB), L-ascorbic acid (AA) (99.9%), silver nitrate (99%), gold(III) chloride trihydrate (99.9%), sodium oleate (NaOL, >95%), TiCl3 (12%), poly(ethylene glycol) (PEG)-SH (2000), 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), phenazine methosulfate (PMS), N-hydroxysuccinimidyl–dibenzocyclooctyne (NHS–DBCO), dopamine, and 9,10-anthracenediyl-bis(methylene) dimalonic acid (ABDA) were purchased from Sigma-Aldrich. Sodium bicarbonate (NaHCO3, 99%), sodium borohydride (NaBH4, 99%), and hydrochloric acid (HCl) (36.5–38% in water) were purchased from Fisher Scientific. The azide-ICG was bought from Intrace-medical. All of the chemicals were used as received without further purification. The DNA was purchased from Integrated DNA Technologies Inc. DOX hydrochloride was bought from Tokyo Chemical Industry Co. Ltd. NHS–mPEG was bought from Laysan Bio, Inc. Deionized water was used throughout the experiments.
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4

Synthesis and Characterization of Photosensitizers

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Trihexyltetradecylphosphonium chloride (P66614Cl) was purchased from Sigma-Aldrich (St. Louis, MO), and porphyrin (TCPP) was purchased from Tokyo Chemical Industry (Tokyo, Japan). FA was purchased from Calbiochem (San Diego, CA). 1,3-Diphenylisobenzofuran (DPBF) and 9,10-anthracenediylbis(methylene)dimalonic acid (ABDA) were purchased from Sigma-Aldrich. Sodium hydroxide and methylene blue (MB) were purchased from VWR (Radnor, PA). All chemicals were used as received, and only TCPP was modified prior to the IMs synthesis. Triply deionized water (18.2 MΩ cm) was obtained using a Purelab Ultrapure water purification system (ELGA, Woodridge, IL). Dichloromethane (DCM) and ethanol were of ACS grade and purchased from Thermo Fisher (Waltham, MA). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and PBS buffer (pH 7.4) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich. DMSO was filtered through a 0.2 μm polytetrafluoroethylene (PTFE) filter prior to use.
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5

Fabrication of Hyaluronic Acid-Based Hydrogel

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Metronidazole (Pharmaceutical Secondary Standard), vancomycin (Van, >90%), 1-(3-dimethylaminopropyl)−3-ethylcarbodiimide hydrochloride (EDC, >99%), N-hydroxysulfosuccinimide sodium salt (NHS, >98%), hyaluronidase (Hyal, 400 units/mg), fibrinogen, thrombin (~120 units/mg), dimethyl sulfoxide (DMSO, >99.9%), dimethylformamide (DMF, >99.9%), 9, 10-anthracenediyl-bis(methylene)-dimalonic acid (ABDA, >90%), and ethylenediamine (>99.5%) were obtained from Sigma-Aldrich; sodium hyaluronate (200 kDa) was purchased from SunlidaBio; and Ce6 (>99%) was bought from JenKem Technology.
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6

Photosensitizer-based Oxidative Stress Assay

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Rose Bengal sodium salt (RB, MW: 1017.64 g/mol, ≥85%, Sigma, Madrid, Spain), tetrabutylammonium iodide (TBAI, MW: 369.37 g/mol, 98%, Aldrich Chemistry, Madrid, Spain), tetrabutylammonium chloride (TBACl, MW: 277.92 g/mol, ≥97%, Aldrich Chemistry, Madrid, Spain), Sodium iodide (NaI, Fisher Chemical, Madrid, Spain), 2-hydroxyethyl methacrylate (HEMA, MW: 130.14 g/mol, 97%, Acros Organics, Madrid, Spain), poly(ethylene glycol) dimethacrylate average Mn 550 (PEGDMA, Aldrich Chemistry, Madrid, Spain), 2,2′-azo-bis-isobutironitrile (AIBN, 98%, Merck, Madrid, Spain), 9,10-anthracenediyl-bis(methylene) dimalonic acid (ABDA, ≥90% (HPLC)), sodium phosphate monobasic dihydrate (≥99%, Sigma-Aldrich, Madrid, Spain), sodium phosphate dibasic anhydrous (≥99%, Fluka, Madrid, Spain), catalase from bovine liver (aqueous suspension, 10.000–40.000 units/mg protein, Sigma-Aldrich, Madrid, Spain), ammonium iron (II) sulfate hexahydrate (for analysis, Thermo Scientific, Madrid, Spain), xylenol orange sodium salt (Thermo Scientific, Madrid, Spain), sorbitol (≥97%, Fisher, Madrid, Spain), sulphuric acid (96%, Merck, Madrid, Spain) and hydrogen peroxide (for analysis, 35 wt.% solution in water, stabilized, Thermo Scientific, Madrid, Spain). All reagents were used as received.
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7

Synthesis and Characterization of Inorganic Nanoparticles

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Indium (III) nitrate hydrate (In(NO3)3 ·xH2O, 99.99%), glutathione (GSH, 98%), silver nitrate (AgNO3, 99%), sodium sulfide nanohydrate (Na2S·9H2O, 99.99%), zinc acetate dehydrate (Zn(Ac)2·2H2O, 98%) and 9, 10-anthracenediyl-bis (methylene) dimalonic acid (ABMDMA) were purchased from Sigma Aldrich (USA). Rhodamine 6G (99%) was purchased from CDH, India. All these were used as received.
Media chemicals (Yeast extract, Peptone, Glucose and Agar) for culture of the microbial cells were procured from HI-Media (Mumbai, India) and Fisher Scientific (Mumbai, India). Analytical grade Sodium chloride, Potassium hydroxide, Disodium hydrogen orthophosphate and Sodium dihydrogen phosphate obtained from Qualigens, India were used for preparing buffers. Fluorescent probe 2, 7, DichlorofluorescinDiacetate (DCFDA) was obtained from Sigma (USA). All the procedures were performed at room temperature 25 °C, unless synthesis otherwise stated.
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8

Synthesis and Characterization of Ce6-ABDA Conjugate

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Bovine Serum Albumin (BSA) and 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) were purchased from Sigma Aldrich Co., Ltd. Ce6, dopamine hydrochloride, tetrahydrofuran (THF), and dimethyl sulfoxide (DMSO) were supplied by Shanghai Maclin Biochemical Technology Co., Ltd. Tris-buffer was purchased from Shanghai Sangon Biotech Co., Ltd. All chemicals were used without further purification.
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9

Barium Titanate Nanoparticles for Photodynamic Therapy

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Barium titanate nanoparticles, rose Bengal, poly(allylamine hydrochloride) (PAH, MW: 15 000), 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA), 1,3-diphenylisobenzofuran (DPBF), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) and Cell Counting Kit-8 (CCK-8) assay were purchased from Sigma-Aldrich (Shanghai, China), Dulbecco's Modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were bought from Gibco. Annexin V and propidium iodide (PI) were purchased from BioLegend. Phosphate buffer saline (PBS), dimethyl sulfoxide and deionized water were obtained from the Chemical Reagent Department of Zhejiang University.
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10

Synthesis and Characterization of Supramolecular Complexes

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Poly-l-lysine (PLL was purchased from Peptide Institute, Inc. (Osaka, Japan). PGA, γ-Cyclodextrin (γ-CDx), 2,3,6-tri-O-methyl-β-cyclodextrin (TMe-β-CDx), and pyrene (Py) were purchased from FUJIFILM Wako Pure Chemical Industries Ltd (Tokyo, Japan). Col was purchased from Nacalai Tesque (Kyoto, Japan). Pullulan (Pul), tetraphenylporphyrin (TPP), 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin (THPP), 5,10,15,20-tetrakis(4-aminophenyl)porphyrin (TAPP), tetraphenylethylene (TPE), α-quinquethiophene (5T), carbamazepin (CBZ), and phthalocyanine (Pc) were purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). [C60]fullerene (C60) was purchased from Frontier Carbon (Tokyo, Japan). Coumarin 6 (C6) and 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) were purchased from Sigma-Aldrich (St Louis, MO, USA). Colon26 cells were maintained in Dulbecco's Modified Eagle Medium containing 10% fetal bovine serum and 1% penicillin–streptomycin.
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