Annexin a1 anxa1

Manufactured by Cell Signaling Technology

Annexin-A1 (ANXA1) is a calcium-binding protein that plays a role in cellular processes, including inflammation, cell signaling, and apoptosis. It is a member of the annexin family of proteins.

Automatically generated - may contain errors

Lab products found in correlation

Criterion tgx precast protein gel, by Bio-Rad (2 mentions) Immun blot pvdf membrane, by Bio-Rad (2 mentions) Ripa buffer, by Merck Group (2 mentions) Anti gapdh, by Cell Signaling Technology (2 mentions) Halt protease inhibitors cocktail, by Thermo Fisher Scientific (2 mentions) Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (2 mentions) Prism 8, by GraphPad (2 mentions) Chemidoc mp imaging system, by Bio-Rad (2 mentions) Nlrp3, by LSBio (1 mentions) Il 1β, by LSBio (1 mentions)

2 protocols using annexin a1 anxa1

Hippocampal Protein and ATP Measurement

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Mouse hippocampi were lysed in radioimmunoprecipitation assay (RIPA) buffer (R0278; Sigma) containing Halt Protease Inhibitors Cocktail (78430; Thermo Scientific). Proteins were separated on 4%-15% or 4%-20% Criterion TGX precast protein gels (64134751; Bio-Rad), and transferred to an Immun-Blot PVDF membrane (1620177; Bio-Rad). After blocking with 5% nonfat dry milk (170-6404; Bio-Rad), the blot was probed with the following antibodies (1:1000): NLRP3 (LS-C374964; LSBio), ASC (67824S; Cell Signaling), Caspase1 (Cleaved Asp210) (LS-C380449; LSBio), IL-1β (LS-C104813; LSBio), and Annexin-A1 (ANXA1) (3299S; Cell Signaling) or (71-3400; Invitrogen). The proteins were visualized using SuperSignal West Dura Extended Duration Substrate (34076; Thermo Scientific) on a ChemiDoc MP Imaging System (Bio-Rad). Protein loading was assessed using anti-GAPDH (2118S; Cell Signaling). Bands were quantified with Fiji software, and analyzed with Prism 8 (GraphPad Software). Hippocampal levels of ATP were determined using a commercially available assay kit (ab83355, Colorimetric/Fluorometric) following the manufacturer’s instructions.

Zhang Z., Ma Q., Velagapudi R., Barclay W.E., Rodriguiz R.M., Wetsel W.C., Yang T., Shinohara M.L, & Terrando N. (2022). Annexin-A1 Tripeptide Attenuates Surgery-Induced Neuroinflammation and Memory Deficits Through Regulation the NLRP3 Inflammasome. Frontiers in Immunology, 13, 856254.

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Hippocampal Protein Expression and ATP

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse hippocampi were lysed in radioimmunoprecipitation assay (RIPA) buffer (R0278; Sigma) containing Halt Protease Inhibitors Cocktail (78430; Thermo Scientific). Proteins were separated on 4%-15% or 4%-20% Criterion TGX precast protein gels (64134751; Bio-Rad), and transferred to an Immun-Blot PVDF membrane (1620177; Bio-Rad). After blocking with 5% nonfat dry milk (170-6404; Bio-Rad), the blot was probed with the following antibodies: NLRP3 (LS-C374964; LSBio), ASC (67824S; Cell Signaling), Caspase1 (Cleaved Asp210) (LS-C380449; LSBio), IL-1β (LS-C104813; LSBio), and Annexin-A1 (ANXA1) (3299S; Cell Signaling) or (71-3400; Invitrogen). The proteins were visualized using SuperSignal West Dura Extended Duration Substrate (34076; Thermo Scientific) on a ChemiDoc MP Imaging System (Bio-Rad). Protein loading was assessed using anti-GAPDH (2118S; Cell Signaling). Bands were quantified with Fiji software, and analyzed with Prism 8 (GraphPad Software).
Hippocampal levels of ATP were determined using a commercially available assay kit (ab83355, Colorimetric/Fluorometric) following the manufacturer's instructions.

Zhang Z., Ma Q., Velagapudi R., Barclay W.E., Rodriguiz R.M., Wetsel W.C., Shinohara M.L., & Terrando N. (2020). Annexin-A1 tripeptide attenuates surgery-induced neuroinflammation and memory deficits through regulation of the NLRP3 inflammasome.

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