Murine recombinant il1β

Manufactured by Jena Biosciences

Murine recombinant IL1β is a cytokine produced in Escherichia coli. It has a molecular weight of approximately 17.5 kDa.

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Lab products found in correlation

Dmem ham s f12, by Harvard Bioscience (2 mentions)

2 protocols using murine recombinant il1β

Cell Stimulation with IFNα and IL1β

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Cells were seeded with a density of 3.5×10⁵ cells per cavity in a collagen-coated 6-well-plate. For the experiments cells were cultivated under FCS-free conditions in DMEM/Ham’s F-12 (Biochrom) supplemented with 2 mM glutamine and 100 U/ml penicillin/0.1 mg/ml streptomycin (Cytogen). Cells were stimulated with 500 U/ml of murine recombinant IFNα4 (Fig 8A) or IFNαA (S4C Fig) (PBL) with or without 10 ng/ml of murine recombinant IL1β (JenaBioscience) for the time points indicated in the respective figure.

Robichon K., Maiwald T., Schilling M., Schneider A., Willemsen J., Salopiata F., Teusel M., Kreutz C., Ehlting C., Huang J., Chakraborty S., Huang X., Damm G., Seehofer D., Lang P.A., Bode J.G., Binder M., Bartenschlager R., Timmer J, & Klingmüller U. (2020). Identification of Interleukin1β as an Amplifier of Interferon alpha-induced Antiviral Responses. PLoS Pathogens, 16(10), e1008461.

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Murine IFNα and IL1β Stimulation

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Cells were seeded with a density of 3.510 5 cells per cavity in a collagen-coated 6-wellplate. For the experiments cells were cultivated under FCS-free conditions in DMEM/Ham's F-12 (Biochrom) supplemented with 2 mM glutamine and 100 U/ml penicillin/0.1 mg/ml streptomycin (Cytogen). Cells were stimulated with 500 U/ml of murine recombinant IFNα (PBL) with or without 10 ng/ml of murine recombinant IL1β (JenaBioscience) for the time points indicated in the respective figure.

Robichon K., Maiwald T., Schilling M., Schneider A., Willemsen J., Salopiata F., Teusel M., Salopiata, Kreutz C., Ehlting C., Huang J., Chakraborty S., Huang X., Damm G., Seehofer D., Lang P.A., Bode J.G., Binder M., Bartenschlager R., Timmer J., & Klingmüller U. (2020). Identification of Interleukin1β as an Amplifier of Interferon alpha-induced Antiviral Responses.

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