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Shc003 turbo gfp

Manufactured by Merck Group
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The SHC003-Turbo-GFP is a laboratory equipment product developed by Merck Group. It is a high-performance turbine-based instrument designed for the detection and analysis of green fluorescent protein (GFP) in various research applications.

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Lab products found in correlation

Scramble shrna shc002, by Merck Group (2 mentions) Shc003, by Merck Group (2 mentions) Plko 1 backbone, by Merck Group (2 mentions)

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SHC003 (8 citations)

2 protocols using shc003 turbo gfp

1

Cloning FAT1 and ZNF750 ORFs

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The open reading frame (ORF) of human FAT1 and ZNF750 transcripts were generously provided by Timothy Chan's group from Memorial Sloan-Kettering Cancer Center27 (link) and Paul Khavari's group from Veterans Affairs Palo Alto Healthcare System24 (link), respectively. Both ORFs were sub-cloned into lentiviral based expression vector SHC003 (Sigma-Aldrich) using Nhe I and Fse I cloning sites. SHC003-Turbo-GFP was used as control (Sigma-Aldrich). The lentiviral based shRNA vectors (shXPO1 and shFAT2 sequences are listed in Supplementary Table 13b) were generated with PLKO.1 backbone (Sigma-Aldrich) using Age I and EcoR I cloning sites. SHC002-Scramble shRNA was used as control (Sigma-Aldrich). The cloning primers are listed in Supplementary Table 13c.
Lin D.C., Hao J.J., Nagata Y., Xu L., Shang L., Meng X., Sato Y., Okuno Y., Varela A.M., Ding L.W., Garg M., Liu L.Z., Yang H., Yin D., Shi Z.Z., Jiang Y.Y., Gu W.Y., Gong T., Zhang Y., Xu X., Kalid O., Shacham S., Ogawa S., Wang M.R, & Koeffler H.P. (2014). Genomic and molecular characterization of esophageal squamous cell carcinoma. Nature genetics, 46(5), 467-473.
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2

Cloning FAT1 and ZNF750 ORFs

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The open reading frame (ORF) of human FAT1 and ZNF750 transcripts were generously provided by Timothy Chan's group from Memorial Sloan-Kettering Cancer Center27 (link) and Paul Khavari's group from Veterans Affairs Palo Alto Healthcare System24 (link), respectively. Both ORFs were sub-cloned into lentiviral based expression vector SHC003 (Sigma-Aldrich) using Nhe I and Fse I cloning sites. SHC003-Turbo-GFP was used as control (Sigma-Aldrich). The lentiviral based shRNA vectors (shXPO1 and shFAT2 sequences are listed in Supplementary Table 13b) were generated with PLKO.1 backbone (Sigma-Aldrich) using Age I and EcoR I cloning sites. SHC002-Scramble shRNA was used as control (Sigma-Aldrich). The cloning primers are listed in Supplementary Table 13c.
Lin D.C., Hao J.J., Nagata Y., Xu L., Shang L., Meng X., Sato Y., Okuno Y., Varela A.M., Ding L.W., Garg M., Liu L.Z., Yang H., Yin D., Shi Z.Z., Jiang Y.Y., Gu W.Y., Gong T., Zhang Y., Xu X., Kalid O., Shacham S., Ogawa S., Wang M.R, & Koeffler H.P. (2014). Genomic and molecular characterization of esophageal squamous cell carcinoma. Nature genetics, 46(5), 467-473.
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