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On targetplus smartpool human sirnas

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ON-TARGETplus SMARTpool human siRNAs are a collection of 4 individual siRNA duplexes designed to target a specific human gene. The siRNAs are provided as a pool to increase the likelihood of effective gene knockdown.

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Lab products found in correlation

On targetplus non targeting sirna, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Bovine tsh, by Merck Group (1 mentions) Gf 109203x gfx, by Enzo Life Sciences (1 mentions) Ly2228820, by Selleck Chemicals (1 mentions) Pertussis toxin ptx, by Thermo Fisher Scientific (1 mentions) Mapk1, by Thermo Fisher Scientific (1 mentions) On targetplus non targeting pool sirna, by Thermo Fisher Scientific (1 mentions) Antibody to β actin, by Merck Group (1 mentions) Forskolin fsk, by Merck Group (1 mentions) U0126, by Selleck Chemicals (1 mentions)

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SiRNAs (258 citations) ON-TARGETplus SMARTpool (112 citations) SMARTpool (46 citations) ON-TARGETplus siRNA (32 citations) ON-TARGETplus (23 citations) SMARTpool siRNAs (19 citations)

6 protocols using on targetplus smartpool human sirnas

1

Nrf2 and AhR Silencing Assay

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The ON-TARGETplus SMARTpool human siRNAs against Nrf2 (L-004018-00-0020), AhR (L-004990-00-0020), and ON-TARGETplus nontargeting siRNA (D-001810-10-05) were provided by Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Cells were transfected with the indicated siRNAs at 50 nM for 24 h using the DharmaFECT transfection agent (Dharmacon Research, Lafayette, CO, USA), according to the manufacturer's protocols.
Lee S.E., Park S.H., Yoo J.A., Kwon K., Kim J.W., Oh S.W., Park S.J., Kim J., Yu E., Han B.S., Cho J.Y, & Lee J. (2019). Antagonizing Effects of Clematis apiifolia DC. Extract against Benzo[a]pyrene-Induced Damage to Human Keratinocytes. Oxidative Medicine and Cellular Longevity, 2019, 2386163.
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2

Targeting HIF-1α and HIF-2α in Cells

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ON-TARGETplus SMARTpool human siRNAs against HIF-1α (L-004018-00-0020), HIF-2α (L-004814-00-0020), and ON-TARGETplus non-targeting siRNA (D-001810-10-05) were synthesized by Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Cells were then transfected with the indicated siRNAs at 50 nM for 48 h using DharmaFECT transfection agent (Dharmacon Research, CO, USA) according to the manufacturer’s instructions.
Jung K., Cho J.Y., Soh Y.J., Lee J., Shin S.W., Jang S., Jung E., Kim M.H, & Lee J. (2015). Antagonizing Effects of Aspartic Acid against Ultraviolet A-Induced Downregulation of the Stemness of Human Adipose Tissue-Derived Mesenchymal Stem Cells. PLoS ONE, 10(4), e0124417.
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3

Signaling Pathway Modulation in Thyroid Cells

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U0126, H-89, and LY2228820 were purchased from Selleckchem; GF109203X (GFX) and phorbol 12, 13-dibutyrate from Enzo Life Sciences. Pertussis toxin (PTX) was obtained from Invitrogen; forskolin (FSK) from Sigma-Aldrich. Bovine TSH was from Sigma-Aldrich. M22 thyroid-stimulating antibodies (TSAbs) were from KRONUS (ID 83669; Star). Thyrostimulin was kindly provided by ZymoGenetics, Inc. Tissue-nonspecific anti-ALPL antibody was from Abcam, Inc (diluted 1:2000). The antibody to β-actin was from Sigma-Aldrich. RDye 680 or 800 secondary antibodies were from Li-Cor. GNAS, GNAQ, GNA11, GNAO, GNAI1–3, MAPK1, MAPK3, and MAPK14 ON-TARGET plus SMART pool human siRNAs or ON-TARGET plus nontargeting pool siRNA (scrambled) were from Thermo Fisher Scientific, Inc.
Boutin A., Neumann S, & Gershengorn M.C. (2016). Multiple Transduction Pathways Mediate Thyrotropin Receptor Signaling in Preosteoblast-Like Cells. Endocrinology, 157(5), 2173-2181.
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4

Silencing HIF-1α and HIF-2α in Cells

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The ON-TARGETplus SMARTpool human siRNAs against HIF-1α (L-004018-00-0020), HIF-2α (L-004814-00-0020), and ON-TARGETplus non-targeting siRNA (D-001810-10-05) were synthesized by Thermo Fisher Scientific, Inc. (Waltham, MA, USA). The expression plasmid for HIF-1α was purchased from OriGene (Rockville, MD, USA). The cells were then transfected with the indicated siRNAs at 50 nM, or along with the HIF-1α-expression plasmid for 48 h using the DharmaFECT transfection agent (Dharmacon Research, CO, USA), according to the manufacturer’s instructions.
Hwang Y.S., Park S.H., Kang M., Oh S.W., Jung K., Park Y.S, & Lee J. (2017). Stemness and differentiation potential-recovery effects of sinapic acid against ultraviolet-A-induced damage through the regulation of p38 MAPK and NF-κB. Scientific Reports, 7, 909.
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5

Transient and Stable Knockdown of Lipid Kinases

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For transient knockdown experiments, cells were seeded in 6-well plates and transfected with 100 nM ON-TARGETplus Human SMARTpool siRNAs (Thermo Scientific) targeting PIKFYVE (Cat.# L-005058-00-0005), PIP5K1C (Cat.# L-004782-00-0005), PIK3CA (Cat.# L-003018-00-0005), or non-targeting control pool (Cat.# D-001810-10-50) using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer’s instructions. For stable doxycycline-inducible shPikfyve Myc-CaP cells, a SMARTvector lentiviral shRNA construct encoding a Pikfyve targeting sequence (TGGTGTCTGCGCCTAAATG) was used to infect Myc-CaP cells, and positively-infected cells were selected by puromycin.
Qiao Y., Choi J.E., Tien J.C., Simko S.A., Rajendiran T., Vo J.N., Delekta A.D., Wang L., Xiao L., Hodge N.B., Desai P., Mendoza S., Juckette K., Xu A., Soni T., Su F., Wang R., Cao X., Yu J., Kryczek I., Wang X.M., Wang X., Siddiqui J., Wang Z., Bernard A., Fernandez-Salas E., Navone N.M., Ellison S.J., Ding K., Eskelinen E.L., Heath E.I., Klionsky D.J., Zou W, & Chinnaiyan A.M. (2021). Autophagy Inhibition by Targeting PIKfyve Potentiates Response to Immune Checkpoint Blockade in Prostate Cancer. Nature cancer, 2, 978-993.
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6

Transient and Stable Knockdown of Lipid Kinases

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For transient knockdown experiments, cells were seeded in 6-well plates and transfected with 100 nM ON-TARGETplus Human SMARTpool siRNAs (Thermo Scientific) targeting PIKFYVE (Cat.# L-005058-00-0005), PIP5K1C (Cat.# L-004782-00-0005), PIK3CA (Cat.# L-003018-00-0005), or non-targeting control pool (Cat.# D-001810-10-50) using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer’s instructions. For stable doxycycline-inducible shPikfyve Myc-CaP cells, a SMARTvector lentiviral shRNA construct encoding a Pikfyve targeting sequence (TGGTGTCTGCGCCTAAATG) was used to infect Myc-CaP cells, and positively-infected cells were selected by puromycin.
Qiao Y., Choi J.E., Tien J.C., Simko S.A., Rajendiran T., Vo J.N., Delekta A.D., Wang L., Xiao L., Hodge N.B., Desai P., Mendoza S., Juckette K., Xu A., Soni T., Su F., Wang R., Cao X., Yu J., Kryczek I., Wang X.M., Wang X., Siddiqui J., Wang Z., Bernard A., Fernandez-Salas E., Navone N.M., Ellison S.J., Ding K., Eskelinen E.L., Heath E.I., Klionsky D.J., Zou W, & Chinnaiyan A.M. (2021). Autophagy Inhibition by Targeting PIKfyve Potentiates Response to Immune Checkpoint Blockade in Prostate Cancer. Nature cancer, 2, 978-993.
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