FFPE tissue specimens were used for IHC analysis. After deparaffinization with xylene and rehydration with an alcohol-graded solution, IHC was performed using a Ventana Discovery XT Automated Slide Stainer (Ventana Medical System, Oro Valley, AZ, USA). Cell conditioning 1 buffer (citrate buffer, pH 6.0; Ventana Medical System) was used for antigen retrieval. Sections were incubated with primary antibodies against PD-L1 (1:50; clone 22C3; DAKO, Agilent Technologies, Santa Clara, CA, USA) and HER2 (1:1500; polyclonal; DAKO). For PD-L1 expression, CPS (1:50; clone 22C3; DAKO) was calculated as previously described (13 (link)). HER2 IHC was assessed according to the American Society of Clinical Oncology/College of American Pathologists guidelines based on the grading system, ranging from 0 to 3+ (14 (link)).
Ventana discovery xt automated slide stainer
Manufactured by Roche
Sourced in United States
The Ventana Discovery XT Automated Slide Stainer is a laboratory equipment used for the automated staining of tissue samples on microscope slides. It is designed to perform various staining protocols, including immunohistochemistry and in situ hybridization, in a consistent and reproducible manner.
Automatically generated - may contain errors
4 protocols using ventana discovery xt automated slide stainer
1
IHC Analysis of FFPE Tissue Samples
2
Immunohistochemical Analysis of Tumor Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
As previously described [21 (link)], 3-μm thick tissue sections were cut from formalin-fixed paraffin-embedded (FFPE) tissue microarray (TMA) blocks. After deparaffinization and rehydration with xylene and alcohol graded solutions, respectively, immunohistochemistry (IHC) was performed by using a Ventana Discovery XT Automated Slide Stainer (Ventana Medical System, Tucson, AZ, USA). Cell Conditioning 1 buffer (citrate buffer, pH 6.0; Ventana Medical System) was used for antigen retrieval. The slices were incubated with primary antibody against p53 (1:300, clone DO-7; Novocastra, Leica Biosystems, Newcastle Upon Tyne, UK), YAP1 (1:200, clone 63.7, Santa Cruz Biotechnology, Dallas, Texas, USA), ER (ER; 1:150, clone 6F11; Novocastra), PR (PR; 1:100; clone 16; Novocastra), HER2 (1:1500, DAKO, Glostrup, Denmark, clone polyclonal). The appropriate positive and negative controls were included.
3
Immunohistochemical Analysis of Endometrium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Endometrium samples from operations, hysterectomy, resectoscope, or dilatation and curettage, were assessed by an expert pathologist. Among 50 patients contributing tissue specimen, 29 patients also underwent IHC profiling using formalin-fixed, paraffin-embedded tissue specimens. After deparaffinization with xylene and rehydration with graded alcohol solution, IHC was performed using Ventana Discovery XT Automated Slide Stainer (Ventana Medical System, Tucson, AZ, USA). Cell Conditioning Buffer 1 (citrate buffer, pH 6.0; Ventana Medical System) was used for antigen retrieval. The slices were incubated with primary antibodies against MLH1 (dilution 1:50, BD Biosciences, San Jose, CA, USA), MSH2 (dilution 1:200, BD Biosciences), MSH6 (dilution 1:100, Cell Marque Corporation, Rocklin, CA, USA), PMS2 (dilution 1:40, Cell Marque), and p53 (dilution 1:50, Dako, CA, USA). IHC stain was scored and interpreted by an expert pathologist (E Park).
4
Immunohistochemistry Protocol for Breast Cancer Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
As described in previous studies [12 (link)], 3 µm thick tissue sections were cut from the TMA block. After deparaffinization and rehydration using xylene and alcohol graded solutions, respectively, immunohistochemistry (IHC) was performed using Ventana Discovery XT Automated Slide Stainer (Ventana Medical System, Tucson, AZ, USA). Cell conditioning 1 buffer (citrate buffer, pH 6.0; Ventana Medical System) was used for antigen retrieval. Appropriate positive and negative controls were included. Staining of all the IHC markers was assessed using light microscopy. A cut-off value of 1% nuclear staining or more was considered positive for ER and PR [13 (link)]. HER2 staining was interpreted based on the 2018 American Society of Clinical Oncology/College of American Pathologists guidelines [14 (link)]. Only strong and circumferential membranous HER2 expression (3+) was considered positive, while 0 and 1+ HER2 staining was considered negative. Cases with equivocal HER2 expression (2+) were further evaluated for HER-2 gene amplification using silver in situ hybridization.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!