Pe conjugated f4 80

Manufactured by Cytek Biosciences

Sourced in United States

PE-conjugated F4/80 is a fluorescently labeled antibody that binds to the F4/80 antigen, a glycoprotein expressed on the surface of murine macrophages. This product can be used in flow cytometry applications to identify and quantify macrophage populations.

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Lab products found in correlation

2 protocols using pe conjugated f4 80

Immunofluorescence Analysis of Cellular Markers

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HRP-conjugated anti-β-ACTIN antibody, Filipin and 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Sigma-Aldrich/ Merck Millipore. Alexa488-conjugated anti-rabbit IgG, HRP-conjugated anti-rabbit total IgG and light chain specific IgG antibodies were purchased from Jackson ImmunoResearch, USA; PE-conjugated F4/80 was procured from Tonbo Biosciences, USA. Alexa Fluor 660 conjugated CD68 was purchased from ThermoFischer Scientific. Anti-G9a, anti-SIRT6, anti-H3K9me1, anti-H3K9me2, anti-H3K9Ac, anti-Ser33/37/Thr41 phospho-β-CATENIN, anti-Ser9 phospho-GSK-3β, anti-β-CATENIN, anti-NRF2, anti-HO1 and anti-TRXR1 antibodies were obtained from Cell Signaling Technology, USA. Anti-LRP2 antibody was purchased from Santa Cruz Biotechnology, USA; anti-SREBP2 antibody was procured from Abcam, USA; and anti-NQO1 antibody was purchased from Calbiochem, USA.

Prakhar P., Bhatt B., Lohia G.K., Shah A., Mukherjee T., Kolthur-Seetharam U., Sundaresan N.R., Rajmani R.S, & Balaji K.N. (2023). G9a and Sirtuin6 epigenetically modulate host cholesterol accumulation to facilitate mycobacterial survival. PLOS Pathogens, 19(10), e1011731.

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Immunostaining for Cell Signaling

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HRP-conjugated anti-β-ACTIN antibody, Filipin and 4′,6-Diamidino-2phenylindole dihydrochloride (DAPI) were purchased from Sigma-Aldrich/ Merck Millipore. Alexa488-conjugated anti-rabbit IgG, HRP-conjugated anti-rabbit total IgG and light chain specific IgG antibodies were purchased from Jackson ImmunoResearch, USA; PE-conjugated F4/80 was procured from Tonbo Biosciences, USA. Anti-G9a, anti-SIRT6, anti-H3K9me1, anti-H3K9me2, anti-H3K9Ac, anti-Ser33/37/Thr41 phospho-β-CATENIN, anti-Ser9 phospho-GSK-3β, anti-β-CATENIN, anti-NRF2, anti-HO1 and anti-TRXR1 antibodies were obtained from Cell Signaling Technology, USA. Anti-LRP2 antibody was purchased from Santa Cruz Biotechnology, USA; anti-SREBP2 antibody was procured from Abcam, USA; and anti-NQO1 antibody was purchased from Calbiochem, USA.

Prakhar P., Bhatt B., Mukherjee T., Lohia G.K., Kolthur‐Seetharam U., Sundaresan N.R., Rajmani R.S., & Balaji K.N. (2021). G9a and Sirtuin6 epigenetically modulate host cholesterol accumulation to facilitate mycobacterial survival.

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