Lentil protein isolate (LPI) used in this study was generously donated by KeyLeaf Corp. (Saskatoon, SK, Canada), and was produced by alkaline extraction (pH 9.5) followed by isoelectric precipitation (pH 4.5), followed by neutralization and spray drying. κ-Carrageenan (κ-C; Lot#: 0001432063) and ι-carrageenan (ι-C; Batch #: 075K1808) were purchased from Sigma-Aldrich Co. (Oakville, ON, Canada). Maltodextrin (MALTRIN M100, dextrose equivalent of 9.0–12.0) was donated by Grain Processing Corporation (Muscatine, IA, USA). Flaxseed oil, which was produced by cold pressing, was provided from Bioriginal Food and Science Crop. (Saskatoon, SK, Canada). All other chemicals used in this study were of reagent grade and purchased through Sigma-Aldrich Co. (Oakville, ON, Canada). Water was filtered through a Milli-Q purification system (Millipore Corporation, Burlington, MA, USA).
κ carrageenan κ c
Manufactured by Merck Group
Sourced in Canada, United Kingdom
κ-Carrageenan (κ-C) is a naturally derived polysaccharide extracted from red seaweeds. It is a linear sulfated polysaccharide composed of alternating 3-linked β-D-galactose and 4-linked α-D-3,6-anhydrogalactose units. κ-C is commonly used as a thickening, gelling, and stabilizing agent in various industries, including food, cosmetics, and pharmaceuticals.
Automatically generated - may contain errors
3 protocols using κ carrageenan κ c
1
Preparation and Characterization of Lentil Protein Isolate
2
Resveratrol-Loaded Carrageenan Hydrogels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were used as received from commercial suppliers without further purification. Tween 80 (Tw80, oleic acid 70%) was purchased from Riedel-de Haën and Span 80 (Sp80, oleic acid ≤ 60%) from Fluka. κ-carrageenan (κ-C) and gelatin (75% protein) from bovine skin were purchased from Sigma-Aldrich. trans-Resveratrol (RSV, > 98%) was purchased from Cayman Chemical Company. Sodium hydroxide, potassium chloride, and potassium dihydrogen phosphate were purchased from Emsure® ISO. 1 N HCl solution was obtained from Sigma Aldrich, absolute EtOH for analysis from Labchem® International, and MeOH for liquid chromatography from Merck. Millipore water purified was used for all the experiments.
3
Potato Protein Isolate Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Potato protein isolate (PoPI) was kindly provided by Solanic B.V. (Veendam, the Netherlands), and the protein, moisture and ash content was 90 wt. %, 6 wt. % and 4 wt. %, respectively. The composition of PoPI was acquired from material specification form from the supplier. Sodium azide and κ-carrageenan (κC) were purchased from Sigma Aldrich (UK). The unadjusted pH of PoPI and κ-C was 3.6 and 3.55, respectively, measured at a temperature of 20 o C and a biopolymer concentration of 1 wt. %. The oil used was commercially available rapeseed oil. All materials were used without further purification.
The water was passed through a double distillation unit (A4000D, Aquatron, UK).
The water was passed through a double distillation unit (A4000D, Aquatron, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!