Immunohistochemistry staining procedures have been previously described. 2 (link) Tissue slides were incubated with antibodies against p- vascular endothelial-cadherin (p-VE-cadherin) (Cell Signaling, Danvers, MA), endothelial marker CD-31 (R&D Systems, Minneapolis, MN) and smooth muscle actin (SMA) (Sigma-aldrich, St Louis, MO). Slides were then incubated with the appropriate alexa fluorconjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA). A Nikon E800 Eclipse microscope (Nikon, Tokyo, Japan) was used to capture images at x20 magnification in five random fields from each animal (4 animals per group). Image J (Image Processing and Analysis in Jave) was used to analyze vascular density. Capillaries were defined as CD31 positive structures between 10-800 pixels and arteries were defined as smooth muscle actin positive structures with lumens greater than 800-infinity pixels.
P ve cadherin
Manufactured by Cell Signaling Technology
Sourced in United States
P-VE-cadherin is a lab equipment product from Cell Signaling Technology. It is a recombinant protein that represents the extracellular domain of human P-VE-cadherin.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)
Eclipse e800 microscope, by Nikon (1 mentions)
P47phox, by Santa Cruz Biotechnology (1 mentions)
P67phox, by Abcam (1 mentions)
Ve cadherin, by Santa Cruz Biotechnology (1 mentions)
Na k atpase, by Santa Cruz Biotechnology (1 mentions)
Nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Phosphor cav 1, by Cell Signaling Technology (1 mentions)
Phosphor src, by Cell Signaling Technology (1 mentions)
Histone 3, by Cell Signaling Technology (1 mentions)
β tubulin, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
2 protocols using p ve cadherin
1
Vascular density analysis by immunohistochemistry
2
Multifunctional Herbal Therapeutic Granules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MHT granules, consisted of HE granule (39.4%), RC granule (26.3%), SAA granule (15.5%) and RG granule (18.8%), were provided by Guangdong Yi Fang Pharmaceutical Co Ltd (Guangzhou, China). Rhodamine 6G was from Fluka Chemie AG (Buchs, Switzerland). Antibodies against Cav-1, phosphor-Cav-1, Src, phosphor-Src, p-VE-cadherin, β-actin, β-tubulin and Histone-3 were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against JAM-1, Claudin-5, ZO-1, NF-κB p65, Na+/K+-ATPase, NADPH oxidase subunit p47phox and p40phox, VE-cadherin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against Occluding, NADPH oxidase subunit p67phox were purchased from Abcam (Cambridge, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!