Liver tissues were fixed with 4% paraformaldehyde solution, embedded in paraffin blocks and processed by immunohistochemistry staining. Tissue sections were deparaffinized and rehydrated using a graded ethanol series and distilled water, and then treated with 3% H2O2 in methanol for 30 min to block endogenous peroxidase activity. Tissue sections were then rinsed twice for five minutes in phosphate-buffered saline (PBS) and incubated with 10% normal goat serum for 30 min to block non-specific antibody binding. After washing, the samples were incubated with primary antibodies against CYP7A1 (Abcam, ab234982, 1:500), CYP8B1 (Abcam, ab175843, 1:50), CYP27A1 (Abcam, ab126785, 1:250), and CYP7B1 (Abcam, ab175889, 1:100). Sections were then washed in PBS three times and incubated with secondary antibodies. The sections were stained with DAB according to the manufacturer’s protocol, mounted on slides, and photographed using a digital microscope camera (Nikon, Tokyo, Japan). The immunohistochemistry sample images were quantified using Image-Pro Plus software (Media Cybernetics, MD, USA).
Ab175843
Manufactured by Abcam
Ab175843 is a recombinant antibody that targets the protein of interest. It is designed for use in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab126785, by Abcam (2 mentions)
Image pro plus software, by Media Cybernetics (1 mentions)
Ab234982, by Abcam (1 mentions)
Digital microscope camera, by Nikon (1 mentions)
Sc 5776, by Santa Cruz Biotechnology (1 mentions)
Sc 81178, by Santa Cruz Biotechnology (1 mentions)
Ab179515, by Abcam (1 mentions)
Ab131084, by Abcam (1 mentions)
Ab155421, by Abcam (1 mentions)
Ab16097, by Abcam (1 mentions)
Af 401 na, by R&D Systems (1 mentions)
Sc 10789, by Santa Cruz Biotechnology (1 mentions)
Sc 47724, by Santa Cruz Biotechnology (1 mentions)
Ab108362, by Abcam (1 mentions)
2 protocols using ab175843
1
Immunohistochemical Analysis of Liver Cytochrome Enzymes
2
Western Blot Protocol for Protein Detection
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Whole cell lysates were derived from cells or tissues using radioimmunoprecipitation assay (RIPA) lysis buffer. Proteins (20–40 μg) with SDS-loading buffer were loaded onto gels and run on SDS-PAGE; then, proteins were transferred to the polyvinylidene fluoride (PVDF) membrane. The PVDF membranes were incubated with primary antibodies against Pro-caspase-1 (1:2000, #ab179515, Abcam), caspase-1 (1:2000, #ab108362, Abcam), NLRP3 (1:2000, #ab16097, Abcam), IL-1β (1:1000, #AF-401-NA, R&D), HO-1 (1:500, #sc-10789, Santa Cruz), GAPDH (1:500, #sc-47724, Santa Cruz), β-actin (1:500, #sc-81178, Santa Cruz), Tubulin (1:1000, #556321, BD Pharmingen), FXRa (1:2000, #ab187735, Abcam), Cyp8b1 (1:2000, #ab175843, Abcam), Cyp27a1 (1:1000,#ab126785, Abcam), Sult2a1 (1:500, #sc-376629, Santa Cruz), Bsep (1:2000, #ab155421, Abcam), Mrp3 (1:500, #sc-5776, Santa Cruz), Ntcp (1:2000, #ab131084, Abcam), and BAAT (1:2000, #ab83882, Abcam). Then, appropriate horseradish peroxidase (HRP)-linked secondary antibodies were incubated for further 1 hour at room temperature. Luminal and H2O2 substrates were used for chemiluminescence detection.
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