The human oral squamous cell carcinoma cell lines Hep-2 and human lymphatic endothelial cells (hLECs) were obtained from BeNa Culture Collection (Hangzhou, Zhejiang, China). hLEC were cultured with 85% RPMI-1640 (Thermo Fisher Scientific, Waltham, MA, USA) and 15% FBS (Atlas Biologicals), and Hep-2 were maintained in RPMI-1640 (Thermo Fisher Scientific, Waltham, MA, USA) and 10% FBS at 37 °C in a humidified atmosphere containing 5% CO2.
The hLEC cells were plated in a six-well plate and treated with EBM-2 or Hep-2 conditioned medium for 48 h. Then the cells were collected and used to extract protein and RNA for correlation detection (western blot analysis and qRT-PCR).
The hLEC cells were plated in a six-well plate and treated with EBM-2 or Hep-2 conditioned medium for 48 h. Then the cells were collected and used to extract protein and RNA for correlation detection (western blot analysis and qRT-PCR).