The levels of TNF-α, IL-8, and IL-6 were determined with the rat TNF-α ELISA kit (Solarbio), rat IL-8 ELISA kit (Solarbio), and rat IL-6 ELISA kit (Solarbio), respectively, according to the manufacturer’s instructions.
Rat tnf α elisa kit
Manufactured by Solarbio
Sourced in China
The Rat TNF-α ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of rat tumor necrosis factor-alpha (TNF-α) in various biological samples.
Automatically generated - may contain errors
4 protocols using rat tnf α elisa kit
1
Cytokine Expression Quantification
2
Cytokine and Cellular Damage Assays
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The TNF-α, IL-8, and IL-6 levels were determined with the rat TNF-α ELISA kit (Solarbio), IL-8 ELISA kit (Solarbio) and IL-6 ELISA kit (Solarbio) according to the manufacturer’s instructions respectively. LDH release was measured using the LDH assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s recommendations. Samples were analyzed at 450 nm wavelength by microplate reader.
3
Evaluating Inflammatory Markers in Rats
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Before the rats were killed under excessive anesthesia, blood was collected through the abdominal aorta, and then centrifuged at 4°C, retained the supernatant and store at −20°C for later use. TNF‐α and IL‐1β levels and in rat was determined using rat TNF‐α ELISA kit and rat IL‐1β ELISA kit according to manufacturer's instructions (Solarbio).
4
Serum Inflammatory Factors post-MI
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The inhibitory effect of BBR@PLGA@PLT NPs on inflammation from the serum inflammatory factor levels was analyzed in rats on day three after MI. The whole blood of rats was collected, and serum was isolated. Serum levels of inflammatory factors were measured by rat IL-6 ELISA kit (Multisciences, Hangzhou, China), rat TNF-α ELISA kit (Solarbio, Beijing, China), and rat IL-10 ELISA kit (ELK biotechnology, Wuhan, China) according to the kit instructions.
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