The antibodies used in immunofluorescence and western blot included Ninj2 (R&D systems, Cat# AF5056), Cleaved-Caspase 3 (Cell Signaling Technology, Cat# 9661), phosphorylated MLKL (abcam, Cat# ab196436), Laminin 211 (abcam, Cat# ab7463), Lama2 (Boster, Cat# BA3201-2), Sox10 (abcam, Cat# ab155279), Sox10 (abcam, Cat# ab216020), Mpz (abcam, Cat# ab31851), Neurofilament (abcam, Cat# ab8135), Ki67 (Gene Tex, Cat# GTX16667), phosphorylated FAK (abcam, Cat# ab4792), GAPDH (Proteintech, Cat# 60004-1-lg), HA (Santa Cruz, Cat# sc-7392), and FLAG (sigma, Cat# B3111).
Phosphorylated fak
Manufactured by Abcam
Sourced in United Kingdom
Phosphorylated FAK is a protein that has been phosphorylated on specific tyrosine residues of the Focal Adhesion Kinase (FAK) enzyme. FAK is a key regulator of cell adhesion and signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Sox10, by Abcam (1 mentions)
Phosphorylated mlkl, by Abcam (1 mentions)
Ninj2, by R&D Systems (1 mentions)
Flag tag (flag), by Merck Group (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Gapdh, by Proteintech (1 mentions)
Laminin, by Abcam (1 mentions)
Integrin β4, by Abcam (1 mentions)
Integrin α6, by Abcam (1 mentions)
β actin, by Abcam (1 mentions)
3 protocols using phosphorylated fak
1
Immunodetection of Neural Markers
2
Immunohistochemical Analysis of NSCLC
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The sections of NSCLC tissues were dewaxed, rehydrated, quenched of endogenous peroxidase, blocked by 5% BSA, and incubated with the primary antibody laminin (1:200; Abcam, Cambridge, UK) or phosphorylated FAK (1:200, Abcam, Cambridge, UK) at 4°C overnight, followed by secondary antibody (Thermo, MA, USA) incubation and counter-staining with hematoxylin. The intensity of protein expression was calculated by IPP pro software.
3
Integrin and Hippo Pathway Protein Expression
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A549 and NCI-H1299 cell lysates were separated on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE). The primary antibodies used were against human proteins integrin α6 (1:1000, Abcam, Cambridge, UK), integrin β4 (1:1000, Abcam, Cambridge, UK), YAP (1:1000, Abcam, Cambridge, UK), phosphorylated YAP (1:1000, Abcam, Cambridge, UK), TAZ (1:1000, Abcam, Cambridge, UK), FAK (1:1000, Abcam, Cambridge, UK), phosphorylated FAK (1:1000, Abcam, Cambridge, UK), and β-actin (1:1000, Abcam, Cambridge, UK) was used as control. Each experiment was performed three times independently.
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