Oro staining kit

Manufactured by Solarbio

Sourced in China

The ORO staining kit is a laboratory tool used to stain neutral lipids and lipoproteins. It provides a standard method for the visualization and quantification of lipid content in samples.

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Lab products found in correlation

Gel imager, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Sartorius (1 mentions) Inverted fluorescence microscope, by Zeiss (1 mentions) Surescript first strand cdna synthesis kit, by GeneCopoeia (1 mentions) Uv spectrophotometer, by Allsheng (1 mentions) Endofectin max reagent, by GeneCopoeia (1 mentions) Masson trichrome staining reagent, by Solarbio (1 mentions) Immobilon western hrp substrate luminol reagent, by Affinity Biosciences (1 mentions) Acrylamide bis solution, by Solarbio (1 mentions) Hieff quick exosome isolation kit plus, by Yeasen (1 mentions) Hematoxylin, by Solarbio (1 mentions) Prism 6, by GraphPad (1 mentions) Infinite 200 pro, by Tecan (1 mentions) Light microscopy, by Olympus (1 mentions) Inverted research microscope, by Nikon (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Bca protein assay kit, by Beyotime (1 mentions)

6 protocols using oro staining kit

Adipocyte Differentiation Protocol

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Fetal Bovine Serum (Bio-Channel, BC-SE-FBS07), DMEM/F-12 (BiologicalIndustries, 01-170-1A), EDTA-Trypsin (Biosharp, BL512A), EndoFectin™Max Reagent (GeneCopoeia, EF013), Cell Value Added-Toxicity Assay Kit (Biosharp, BS350B), PA (Aladdin, S161420), OA (Maclean, S817542), ORO Staining Kit (Solarbio, G1262), BlazeTaq™ SYBR®Green Mix (GeneCopoeia, QP031), Dnase QP031), BCA Protein Reagent (P0009-1, P0009-1), Hematoxylin (Solarbio, H8070), Eosin Staining Reagent (Beyotime, C0105-2), Masson Trichrome Staining Reagent (Solarbio, G1340), TRIzol®Reagent (Life, Cat. no. 15596-018), SureScript™ First-Strand cDNA Synthesis Kit (GeneCopoeia, QP056), Immobilon Western HRP Substrate Luminol Reagent (Affinity, KF001), 30% Acrylamide/Bis solution (29:1) (Solarbio, A1010), 24-well plate (VIRYA, 3512409), FBS(Bio-Channel, BC-SE-FBS07), Hieff® Quick exosome isolation kit Plus (YEASON, China).
Tissue embedding kit (Jiangsu Shitai, 20084), Real-timePCR (Thermo Fisher Scientific, TCR0096, USA), UV spectrophotometer (ALLSHENG, USA), benchtop low-speed centrifuge (Weil Ltd., China), inverted fluorescence microscope (Zeiss, Observer.A1, Germany), gel imager (Monad, USA), ice maker (FM150KE, China), 4 °C refrigerator (Haier, China), baking machine (Thermo Scientific, DB-B2, USA), TEM(ZCIBIO, China), NTA(Particle Metrix, China).

Liang C., Gao S., Gao J., Xu Y, & Li Q. (2023). Comparison of effects of HucMSCs, exosomes, and conditioned medium on NASH. Scientific Reports, 13, 18431.

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Evaluating Transdifferentiated Lipid Droplets

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To evaluate the transdifferentiation BSCs, after 96 h of differentiation, the
cells were fixed with Oil Red O (ORO) Fixative for 30 min and stained with
hematoxylin staining solution for 2 min at ambient temperature. The staining
protocol was performed according to the instructions provided by the ORO
staining kit (G1262, Solarbio, Beijing, China). Images were collected using an
inverted microscope (IX-73, Olympus) and 20 different stained areas were
captured. Image analysis followed the protocol of Deutsch et al. [6 (link)] using the ImageJ software 1.43u
(http://rsbweb.nih.gov/ij). The average lipid droplet size was
evaluated using GraphPad Prism 6.07.

Zhang J., Li Q., Yan Y., Sun B., Wang Y., Tang L., Wang E., Yu J., Nogoy K.M., Li X, & Choi S.H. (2021). Effect of ciglitazone on adipogenic transdifferentiation of bovine skeletal muscle satellite cells. Journal of Animal Science and Technology, 63(4), 934-953.

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Oil Red O Staining of Bioprinted Constructs

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Oil Red staining were conducted by the instruction of Oil Red O (ORO) staining kit (G1262, Solarbio, Beijing, China). Briefly, 15 μm sections of bioprinted constructs were freshly cut by freezing microtome. Slices were fixed by ORO Fixative for 10–15 min, and put in ventilated space for another 10 min. Immersed the slices into freshly prepared ORO Stain for 15 min, then washed by isopropanol for 20–30 s, and finally washed by distilled water thoroughly. Nuclear was counterstained with Mayer hematoxylin for 2 min. Slices were processed with ORO Buffer for 1 min and mounted by glycerine/gelatin mounting medium.

Hu T., Cui X., Zhu M., Wu M., Tian Y., Yao B., Song W., Niu Z., Huang S, & Fu X. (2020). 3D-printable supramolecular hydrogels with shear-thinning property: fabricating strength tunable bioink via dual crosslinking. Bioactive Materials, 5(4), 808-818.

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Myocardial Lipid Deposition Analysis

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Myocardial lipid depositions were assessed to investigate the metabolic condition of the hearts using the ORO staining kit (Solarbio; G1261) according to the manufacturer's instructions. ImageJ was used to quantify lipid depositions detected at X40 magnification.

Ndzie Noah M.L., Mprah R., Wowui P.I., Adekunle A.O., Adu-Amankwaah J., Tan R., Gong Z., Li T., Fu L., Machuki J.O., Zhang S, & Sun H. (2024). CD73/adenosine axis exerts cardioprotection against hypobaric hypoxia-induced metabolic shift and myocarditis in a sex-dependent manner. Cell Communication and Signaling : CCS, 22, 166.

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Quantifying Intracellular Lipid Droplets

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Lipid droplets in cells were stained with ORO staining kit (Solarbio, Beijing, China). HepG2 cells were seeded on chamber slides. After treatment for 24 h, cells were fixed with 4% buffered paraformaldehyde for 30 min and washed for three times with PBS. The slides were stained according to the manufacturer’s instructions. Morphological changes were observed under light microscopy (Olympus, Tokyo, Japan). Positive area was measured using Image J software (Version 1.52, National Institutes of Health, United States). The extent of intracellular oil lipids was measured as described by Cui et al. (2010) (link). ORO was extracted by isopropanol. The absorbance was measured at a wavelength of 405 nm by using a microplate reader (Infinite 200 PRO, TECAN, Switzerland).

Li R., Bu Y., Yang C, & Wang J. (2021). Effects of Lipid Deposition on Viscoelastic Response in Human Hepatic Cell Line HepG2. Frontiers in Physiology, 12, 684121.

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Quantitative Lipid Droplet Analysis in Adipocytes

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An ORO staining kit (Solarbio) was used to stain lipid droplets in mature 3T3-L1- and C2C12-derived adipocytes. In brief, cells were washed twice with PBS and fixed with ORO fixative at room temperature for 20 min. Then, cells were rinsed with 60% isopropyl alcohol and stained with ORO dye for 20 min. After the ORO dye was washed away with distilled water, ORO-stained cells were visualized with an inverted research microscope (Nikon). For quantification, intracellular ORO was extracted with 100% isopropanol and quantified by measuring the optical absorbance at 520 nm using a microplate reader (BioTek Instruments). Then, the total protein was detected by a BCA protein assay kit (Beyotime) at 562 nm with a microplate reader (BioTek Instruments). The relative ORO value was calculated as OD_ORO-520 values to total protein level.

Yu W., Chen C.Z., Peng Y., Li Z., Gao Y., Liang S., Yuan B., Kim N.H., Jiang H, & Zhang J.B. (2021). KRAS Affects Adipogenic Differentiation by Regulating Autophagy and MAPK Activation in 3T3-L1 and C2C12 Cells. International Journal of Molecular Sciences, 22(24), 13630.

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