HeLa and hTERT-RPE1 cells were grown as previously described.10 (link) A2780, TOM-1 and MOLT-4 cells were grown in RPMI (Sigma-Aldrich, St. Louis, MO, USA) with 2 mM l -glutamine and 10% fetal bovine serum (FBS; GE Healthcare Hyclone, Little Chalfont, Buckinghamshire, UK), MCF-7 cells in Eagle's Minimum Essential Medium (Sigma-Aldrich) with 10% FBS, 2 mM l -glutamine and 1% nonessential amino acids. Phase contrast photographs were performed with a Leica DFC 320 microscope (Leica Microsystems GmbH, Wetzlar, Germany).
Dfc320 microscope
Manufactured by Leica
Sourced in Germany
The Leica DFC320 is a digital microscope camera designed for a wide range of microscopy applications. It features a high-resolution sensor, allowing for the capture of detailed images. The camera is compatible with various Leica microscope models and can be used for documentation, analysis, and imaging purposes.
Automatically generated - may contain errors
Lab products found in correlation
Roswell park memorial institute (rpmi), by Merck Group (1 mentions)
Eagle s minimum essential medium, by Merck Group (1 mentions)
Bovine collagen 1, by Thermo Fisher Scientific (1 mentions)
Hank s balanced salt solution, by Merck Group (1 mentions)
Fd rapid golgistain kit, by FD NeuroTechnologies (1 mentions)
Mz 12.5 stereomicroscope, by Leica (1 mentions)
Application suite version 3, by Leica (1 mentions)
Dm1000 microscope, by Leica (1 mentions)
Dmire2, by Leica (1 mentions)
Qwin standard image analysis, by Leica (1 mentions)
7 protocols using dfc320 microscope
1
Cell Culture Conditions and Imaging
2
Collagen Gel Contraction Assay
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Into 6 well plates 1 × 10 5 cells/well were mixed with a solution of Bovine Collagen I (4 mg/ml; Invitrogen) and Hank's Balanced Salt Solution (1×, pH 7.2; Sigma-Aldrich), seeded and allowed to set at 37°C in a stainless steel washer (135 mm), which was subsequently removed. Baseline 0 h images and day 8 end-point images were acquired using the Leica DFC320 microscope (Leica Microsystems). Gel contraction was measured as gel area at day 8 normalised to the initial gel area of 0 h images using the Fiji distribution of ImageJ.
3
Dendritic Spine Analysis via Golgi Staining
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Golgi staining was performed to detect the dendritic spines of neurons using the FD Rapid Golgi Stain™ Kit (FD Neuro Technologies, Ellicott City, MD) following the manufacturer's instructions. Brain tissue sections of 100-μm thickness were cut, and dehydrated in sequential rinses of 50%, 75%, 95% and 100% ethanol and cleared in xylene. The staining was viewed under a Leica DFC320 microscope. ImageJ software was used to analyze the number of spines and the total dendritic length. Distal dendrites at > 50 µm away from the soma were selected for measurement of spine density.
4
Dissection and Imaging of Aedeagi and Abdominal Sternites
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The aedeagi and abdominal sternites VIII of females were dissected under a stereoscopic microscope, cleared in 10% KOH solution for several minutes, then placed in a droplet of glycerol and examined under a compound light microscope. Photographs of the type specimens were taken with a Leica DFC320 microscope, multiple layers were stacked using CombineZM software. Line drawings were made with the aid of camera lucida attached to a Leica MZ12.5 stereomicroscope. Body length is measured from the anterior margin of the clypeus to the elytral apex, body width is measured across the humeral part of elytra.
Complete label data are listed for type specimens, using square brackets “[ ]” for our remarks and comments, [p] indicating that the following data are printed and [h] that they are handwritten. Quotation marks are used to separate data from different labels and a backslash “\” to separate data from different lines of the same label.
The material is preserved in the following collections:
CAS ;
IZAS ;
MHBU ;
NHMB ;
NMPC ;
SKCR .
Complete label data are listed for type specimens, using square brackets “[ ]” for our remarks and comments, [p] indicating that the following data are printed and [h] that they are handwritten. Quotation marks are used to separate data from different labels and a backslash “\” to separate data from different lines of the same label.
The material is preserved in the following collections:
California Academy of Sciences, San Francisco, USA
Institute of Zoology, Chinese Academy of Sciences, Beijing, China
Museum of Hebei University, Baoding, China
Naturhistorisches Museum Basel, Switzerland
Národní muzeum, Praha, Czech Republic
Sergey V. Kazantsev private collection, Moscow, Russia
5
Fungal Morphological Characterization Protocol
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Morphological characterization of fungal cultures was performed using a Leica DM1000 microscope and fungal tissue was slide mounted for microscopy in distilled water. All measurements were performed in Leica Application Suite version 3 using digital micrographs taken with a Leica DFC320 microscope camera.
6
Histochemical GUS Staining for Seedling Analysis
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Histochemical GUS staining was carried out on seeds and seedlings of pPM19L1:UidA lines, grown on agar plate media as described by 33 . After incubation, the pigments were removed by repeated washing in 70% (v/v) ethanol. Images of GUS-stained seedlings were recorded with a Leica DFC320 microscope. Imaging of GFP-expressing plants was done using a Leica DMIRE2 confocal microscope with an HCV PL APO CS 40X 1.25 oil immersion lens. GFP excitation wavelength was 488 nm, emission 500-550 nm. FM4-64 excitation wavelength was 561 nm and emission 650-740 nm.
7
Ultrastructural Analysis of Cell Samples
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Samples were fixed using 3% (v/v) glutaraldehyde in PBS for 2 hours at RT. Cells were pipetted and rinsed in the fixative during the fixation period. They were then washed three times with phosphate-buffered saline (PBS), 10 min each, dehydrated in an ethanol series, treated for the methylation-acetylation procedure (Testillano, González-Melendi, Ahmadian & Risueño, 1995) and finally embedded in LR White resin (London Resin, EMA, UK). From resin-embedded materials, semi-thin 2 µm thick sections were obtained from the LR White blocks, visualized by phase contrast in a Leica DFC320 microscope equipped with a Leica DM2500 CCD digital camera. Ultrathin sections were stained for 30 min with 5% (w/v) uranyl acetate and for 90 seconds with 0.3% (w/v) lead citrate, separated by a wash in distilled water. Samples were observed using a JEOL 1230 transmission electron microscope operating at 100 kV acceleration voltage. The images obtained were processed for quantitative studies using QWin Standard image analysis (Leica Microsystems) and Image J 2.0 (imagejdev.org) software.
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