Log In Sign up
The largest database of trusted experimental protocols

Hyclone antibiotic antimycotic solution

Manufactured by Thermo Fisher Scientific
Visit Supplier

HyClone Antibiotic Antimycotic Solution is a sterile, stable, and ready-to-use solution that contains a mixture of antibiotics and antimycotics. It is designed to prevent bacterial and fungal contamination in cell culture applications.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Stempro nsc sfm, by Thermo Fisher Scientific (2 mentions) Cycloheximide (chx), by Cell Signaling Technology (1 mentions) Laminin, by Merck Group (1 mentions) Bafilomycin a1, by InvivoGen (1 mentions) Stattic, by Selleck Chemicals (1 mentions) Gibco b 27 serum free supplement, by Thermo Fisher Scientific (1 mentions) Horse serum, by Thermo Fisher Scientific (1 mentions) Gelatin coated cell culture plastic, by BD (1 mentions) Amphotericin b, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)

4 protocols using hyclone antibiotic antimycotic solution

1

Glioblastoma Stem Cell Culture and Drug Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
All GBM tumor samples and subsequent cell line derivations were performed with written consents from patients through Quiñones laboratory, observing Institutional Review Board guidelines as previously described. GBM stem cells with tumorsphere forming capabilities, GBM 276 and GBM 612, were cultured in DMEM/F12 (1:1), 1% HyClone Antibiotic Antimycotic Solution (Thermo Scientific), Gibco B-27 serum free Supplement (Thermo Scientific 17504044), 20 ng/ml EGF, and 20 ng/ml FGF. For ligand treatment experiments, Gibco B27 serum-free supplement, minus insulin (Thermo Scientific A1895601) was used. For adherent culture, dishes were coated with Laminin (Sigma-Aldrich L2020). Coating was done in DMEM/F12 basal media for at least 2 h or overnight, and for every 1 cm2 of surface area, 1 μl of Laminin was added. Drugs used in this study are as follows: Cycloheximide (Cell signaling #2112), Bafilomycin A1 (InvivoGen CAS # 88899-55-2), MG-132 (InvivoGen CAS # 133407-82-6), Cryptotanshinone (Selleckchem Catalog No. S2285), Stattic (Selleckchem Catalog No. S7024), and S3I-201 (Selleckchem Catalog No. S1155).
Ko M., Makena M.R., Schiapparelli P., Suarez-Meade P., Mekile A.X., Lal B., Lopez-Bertoni H., Kozielski K.L., Green J.J., Laterra J., Quiñones-Hinojosa A, & Rao R. (2022). The endosomal pH regulator NHE9 is a driver of stemness in glioblastoma. PNAS Nexus, 1(1), pgac013.
+ Open protocol
+ Expand
2

Glioblastoma Tumor Sample Acquisition and Cell Culture

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
All glioblastoma tumor samples were obtained with written informed consent from patients through the Department of Neurosurgery, Johns Hopkins Hospital (Baltimore, MD), following Institutional Review Board (IRB) guidelines and as previously described2 (link),51 (link),52 (link). Patient tumor grade and type was determined by a neuropathologist (Supplementary Table 1). Culture conditions were in a 5% CO2 incubator at 37°C as follows: GBM 253 primary glioblastoma cells were subcultured in DMEM/F12 (1:1) Nutrient Mixture, 10% fetal bovine serum (Invitrogen), and 1% HyClone Antibiotic Antimycotic Solution (Thermo Scientific). GBM 276 and GBM 612 glioblastoma stem cells were cultured in one of two ways: 1) as serum-free cultures on laminin and poly-L-ornithine coated plates53 (link) using StemPro NSC SFM (Invitrogen) without EGF, or 2) as tumorsphere cultures with EGF (20 ng mL-1). EGF was only added to adherent cell cultures during experimental procedures.
Kondapalli K.C., Llongueras J.P., Capilla-González V., Prasad H., Hack A., Smith C., Guerrero-Cázares H., Quiñones-Hinojosa A, & Rao R. (2015). A Leak Pathway for Luminal Protons in Endosomes Drives Oncogenic Signaling in Glioblastoma. Nature communications, 6, 6289.
+ Open protocol
+ Expand
3

Expansion and Differentiation of C2C12 Myoblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse skeletal myoblasts (C2C12) were purchased from ATCC and expanded in DMEM (Gibco) supplemented with 10% FBS, and 1% HyClone Antibiotic Antimycotic solution (Thermo Scientific) on gelatin coated cell-culture plastic (BD Biosciences). Cells were cultured under standard culture conditions (37°C in 5% CO2). Medium changed every o ther day. To avoid myotube formation, cells were passaged with 0.05% trypsin/EDTA (cellgro-25052CI) at 70 – 80% confluency. For C2C12 differentiation, we supplemented DMEM (Gibco) with 2% horse serum (Gibco) and 1% antibiotic antimycotic solution (10,000 units/ml penicillin, 10,000μg/ml streptomycin, and 25μg/ml Amphotericin B; HyClone). C2C12 were expanded until passage 6 – 10.
Jank B.J., Xiong L., Moser P.T., Guyette J.P., Ren X., Leonard D.A., Fernandez L, & Ott H.C. (2015). Engineered Composite Tissue as a Bioartificial Limb Graft. Biomaterials, 61, 246-256.
+ Open protocol
+ Expand
4

Glioblastoma Tumor Sample Acquisition and Cell Culture

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
All glioblastoma tumor samples were obtained with written informed consent from patients through the Department of Neurosurgery, Johns Hopkins Hospital (Baltimore, MD), following Institutional Review Board (IRB) guidelines and as previously described2 (link),51 (link),52 (link). Patient tumor grade and type was determined by a neuropathologist (Supplementary Table 1). Culture conditions were in a 5% CO2 incubator at 37°C as follows: GBM 253 primary glioblastoma cells were subcultured in DMEM/F12 (1:1) Nutrient Mixture, 10% fetal bovine serum (Invitrogen), and 1% HyClone Antibiotic Antimycotic Solution (Thermo Scientific). GBM 276 and GBM 612 glioblastoma stem cells were cultured in one of two ways: 1) as serum-free cultures on laminin and poly-L-ornithine coated plates53 (link) using StemPro NSC SFM (Invitrogen) without EGF, or 2) as tumorsphere cultures with EGF (20 ng mL-1). EGF was only added to adherent cell cultures during experimental procedures.
Kondapalli K.C., Llongueras J.P., Capilla-González V., Prasad H., Hack A., Smith C., Guerrero-Cázares H., Quiñones-Hinojosa A, & Rao R. (2015). A Leak Pathway for Luminal Protons in Endosomes Drives Oncogenic Signaling in Glioblastoma. Nature communications, 6, 6289.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!