Beckman au5800 system

Ten assays were evaluated using the manufacturer/analyser combinations routinely used in Peking Union Medical College Hospital (Table 1). All reagents and calibrators for the three analysers were obtained from the original manufacturer except for creatinine (CREA; Maccura Biotechnology Co., Ltd., Chengdu, China) and bilirubin, total (BT; Wako Pure Chemical Industries, Ltd., Osaka, Japan) on the Beckman AU5800 system (Beckman Coulter, Inc., Brea, USA).

Blood samples were collected from each patient between 6:00 a.m. and 8:00 a.m. following an overnight fast, and immediately sent to the Laboratory Center of the First Hospital of Shanxi Medical University before 11:00 a.m. on the same day. Then the biomarkers including total cholesterol (TC), high-density lipoproteins (HDL), triglycerides (TG), low-density lipoproteins (LDL), free tri-iodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), thyroid peroxidase antibody (TPOAb), anti-thyroglobulin antibody (TgAb) and fasting blood glucose (FBG) were measured by a BECKMAN AU5800 system (Beckman Coulter, Brea, California, USA).

On the day when the clinical rating scale was measured, blood samples were collected between 6 am and 7 am after an overnight fast. All samples were sent to the laboratory center of the hospital immediately and measurements were taken before 11 am in the morning of the same day. The plasma TC, TG, HDL-c, and LDL-c levels were measured on the BECKMAN AU5800 system (Beckman Coulter, Brea, California, USA).