New brunswick galaxy 170 s

Manufactured by Eppendorf

Sourced in Switzerland

The New Brunswick Galaxy 170 S is a compact and versatile incubator shaker designed for cell culture and microbial applications. It features a temperature range of 4°C to 60°C, an orbital shaking motion, and a capacity of up to 18 liters.

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Galaxy 170S (27 citations) NEW BRUNSWICKTM (4 citations)

5 protocols using new brunswick galaxy 170 s

Chicken Erythroblast Cell Culture Protocol

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6C2 chicken erythroblast cells, transformed by the avian erythroblastosis virus (AEV) carrying a stably integrated mCherry transgene, were maintained in αMinimal Essential Medium (Thermo Fischer Scientific, Basel, Switzerland) complemented with 10% Fetal Bovine Serum (FBS, Life Technologies, Zug, Switzerland), 1% Normal Chicken Serum (Thermo Fischer Scientific, Basel, Switzerland) [32 (link)], 1% penicillin and streptomycin (10,000 U/ml, Thermo Fischer Scientific, Basel, Switzerland), 100 nM β-mercaptoethanol (Sigma-Aldrich, Buchs, Switzerland), and kept at 37°C with 5% CO₂ in an incubator (New Brunswick Galaxy 170 S, Eppendorf, Schönenbuch, Switzerland).
T2EC cells were extracted from the bone marrow of white leghorn chicken embryos (INRA, Tours, France) [33 (link)]. The cells were cultured in αMinimal Essential Medium (Gibco), supplemented with 1 mM HEPES (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS, BioWest), 1% Penicillin-Streptomycin (10,000 U/mL, Gibco), 100 nM β-mercaptoethanol (Sigma-Aldrich), 1 mM dexamethasone (Sigma-Aldrich), 5 ng/mL transforming growth factor-alpha (TGF-α, Peprotech) and 1 ng/mL transforming growth factor-beta (TGF-β, Peprotech), and kept at 37°C with 5% CO₂ in an incubator.

Aslan Kamil M., Fourneaux C., Yilmaz A., Stavros S., Parmentier R., Paldi A., Gonin-Giraud S., deMello A.J, & Gandrillon O. (2023). An image-guided microfluidic system for single-cell lineage tracking. PLOS ONE, 18(8), e0288655.

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hMSC Expansion with Pro293 Medium

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hMSCs (STEMCELL Technology #70071, female) were cultured for 48 h in T75 flasks pre-treated with 0.1% gelatin solution as described by the provider (MilliporeSigma, Burlington, MA, USA) using Pro293 medium (Lonza, Basel, Switzerland) supplemented with 1% GlutaMAX Supplement (Life Technologies, Zug, Switzerland), 1% MesenCult-ACF Plus 500X Supplement (STEMCELL Technologies, Vancouver, Canada) and 1% (v/v) Penicillin-Streptomycin (10,000 U/mL, Life Technologies, Zug, Switzerland) in a CO2 incubator (New Brunswick Galaxy 170 S, Eppendorf, Schönenbuch Switzerland) at 37°C, 5% CO2.

LaPlante E.L., Stürchler A., Fullem R., Chen D., Starner A.C., Esquivel E., Alsop E., Jackson A.R., Ghiran I., Pereira G., Rozowsky J., Chang J., Gerstein M.B., Alexander R.P., Roth M.E., Franklin J.L., Coffey R.J., Raffai R.L., Mansuy I.M., Stavrakis S., deMello A.J., Laurent L.C., Wang Y.T., Tsai C.F., Liu T., Jones J., Van Keuren-Jensen K., Van Nostrand E., Mateescu B, & Milosavljevic A. (2023). exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers. Cell Genomics, 3(5), 100303.

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Cultivating Human Embryonic Kidney Cells

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293T cells (Thermo Fisher Scientific #R78007, female) were cultured for 48 h in CellBIND (Sigma Aldrich, St. Louis, MO, USA) culture dishes using Pro293 medium (Lonza, Basel, Switzerland) supplemented with 1% GlutaMAX Supplement (Life Technologies, Zug, Switzerland) and 1% (v/v) Penicillin-Streptomycin (10,000 U/mL, Life Technologies, Zug, Switzerland) in a CO2 incubator (New Brunswick Galaxy 170 S, Eppendorf, Schönenbuch, Switzerland) at 37°C, 5% CO2.

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EL4 and CT26 Cell Culture Protocol

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EL4, a murine thymoma cell line, was selected as an initial tumor model to test the efficacy of 5-FU loaded PLGA pellets due to the sensitivity of these types of cells to 5-FU.^{[13 (link)]} EL4 cells were obtained from the American Type Culture Collection (ATCC: Manassas, VA) and maintained in RPMI-1640 medium (Gibco, Life technologies, Grand Island, NY). The media was supplemented with 10% v/v fetal calf serum (Atlanta Biologicals, Lawrenceville, GA), 10 mM HEPES (Gibco), 50 μg/ml gentamycin sulfate (Cellgro, Manassas, VA), 1 mM sodium pyruvate (Gibco), 1 mM Glutamax^™ (Gibco) and 0.05 mM 2-mercaptoethanol (Sigma-Aldrich). CT26, a murine colon cancer cell line, was purchased from ATCC. CT26 cells were maintained in RPMI-1640 medium supplemented as described above except that 2-mercaptoethanol was not added. Cells were incubated at 37°C and 5% CO₂ in a humidified incubator (New Brunswick^™ Galaxy^® 170S, Eppendorf, Humburg, Germany).

Leelakanok N., Geary S.M, & Salem A.K. (2017). Antitumor Efficacy and Toxicity of 5-FU-loaded PLGA Pellets. Journal of pharmaceutical sciences, 107(2), 690-697.

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Cultivation of Oral Streptococcal Species

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The strains used in the present study, i.e., S. mutans ATCC 25175 and S. sanguinis ATCC 10556, come from The American Type Culture Collection (ATCC). The strains were inoculated in blood agar plates (OXOID LTD., Basingstoke, Hampshire, UK) and incubated at 37 °C in an incubator with 5% of CO₂ (New Brunswick™ Galaxy® 170S, Eppendorf AG, Hamburg, Deutschland, Germany) to obtain cultures. Subsequently, they were cultivated in Brain Heart Infusion Agar plates (BHA) (OXOID LTD., Basingstoke, Hampshire, UK) under the same conditions mentioned above to refresh colonies.
Both species were routinely grown overnight at 37 °C and 5% CO₂ in TSBs. After this time, each bacterial suspension was adjusted to 82% of transmittance, equivalent to 0.086 of optical density (O.D.), at 492 nm wavelength. A spectrophotometer (Helios epsilon Model, Thermiospectronic, Waltham, MA, USA) was used for the measurement and subsequently, the suspension was diluted 1/100 or 1/10 in TSBs to obtain approximately 10⁶ or 10⁷ CFU/mL, respectively and thus to be used as inocula in the remaining experiments.

Navarro-Pérez M.L., Vadillo-Rodríguez V., Fernández-Babiano I., Pérez-Giraldo C, & Fernández-Calderón M.C. (2021). Antimicrobial activity of a novel Spanish propolis against planktonic and sessile oral Streptococcus spp. Scientific Reports, 11, 23860.

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