Recombinant human granzyme B (Enzo Life Sciences) was mixed with BioPorter Reagent (MilliporeSigma) at 25°C for 5 minutes. The tumor cells were mixed with BioPorter–granzyme B complexes for 4 hours at 37°C. Next, the cells were stained for active caspase-3 as an index of apoptosis and examined by flow cytometry.
Recombinant human granzyme b
Manufactured by Enzo Life Sciences
Sourced in United States
Recombinant human granzyme B is a serine protease enzyme that is expressed in the cytotoxic granules of natural killer cells and cytotoxic T lymphocytes. It plays a key role in the induction of apoptosis in target cells during the immune response.
Automatically generated - may contain errors
3 protocols using recombinant human granzyme b
1
Granzyme B-Induced Apoptosis Assay
2
Granzyme B-Mediated Apoptosis Induction
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Recombinant human granzyme B (Enzo Life Sciences, New York, NY, USA) was mixed with BioPORTER QuikEase Protein Delivery Kit (Sigma-Aldrich, St. Louis, MO, USA). 1 × 105 cells per well were plated in 12-well plates and cultured overnight at 37 °C. Cells were washed, and 100 ng of granzyme B in Opti-MEM (Thermo Fisher Scientific) was added to each well. After incubation for 4 h at 37 °C, the frequency of apoptotic cells was determined by staining with active caspase-3 antibody (BD Biosciences) and examined by flow cytometry as shown gating strategy in Supplementary Figure S1 .
3
Granzyme B-Induced Apoptosis in Tumor Cells
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Recombinant human granzyme B (Enzo Life Sciences) was mixed with BioPorter Reagent (Sigma-Aldrich) at 25 °C for 5 min. Tumor cells were mixed with BioPorter–granzyme B complexes for 4 h at 37 °C. Cells were stained for active caspase-3 as an index of apoptosis and examined by flow cytometry.
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