Anti wipi2

Manufactured by Bio-Rad

Anti-WIPI2 is a laboratory product that targets the WIPI2 protein. WIPI2 is involved in cellular processes related to autophagy. This product can be used in research applications to study the WIPI2 protein and its role in cellular functions.

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Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Anti atg13, by Merck Group (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Anti α tubulin, by Proteintech (1 mentions) Anti ulk1 clone d8h5, by Cell Signaling Technology (1 mentions) Anti atpb clone 3d5, by Abcam (1 mentions)

2 protocols using anti wipi2

Autophagy Signaling Pathway Antibodies

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anti-pS757 ULK1, anti-pS555 ULK1 and anti-ULK1 were from CST, anti-pS318 ATG13 from Novus, anti-ATG13 from Sigma–Aldrich, anti-α-Tubulin from Proteintech and sheep anti-LC3b (S400D) from MRC PPU Reagents and Services. All antibodies were used for Western blotting at 1:1000 dilution apart from the LC3b antibody which was used at 1:500. HRP-conjugated secondary anti-sheep, anti-rabbit and anti-mouse antibodies were purchased from Thermo Scientific and used in 1:10 000 dilution. For immunofluorescence, anti-WIPI2 (Bio-Rad), anti-ULK1 (CST) and anti-LC3 (MBL) antibodies were used in 1:500 dilution. Secondary antibodies for immunofluorescence (anti-mouse Alexa Fluor® 488 and anti-rabbit Alexa Fluor® 594) (Thermo Scientific) were used in 1:1000 dilution.

Zachari M., Rainard J.M., Pandarakalam G.C., Robinson L., Gillespie J., Rajamanickam M., Hamon V., Morrison A., Ganley I.G, & McElroy S.P. (2020). The identification and characterisation of autophagy inhibitors from the published kinase inhibitor sets. Biochemical Journal, 477(4), 801-814.

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Immunoblotting and Immunocytochemistry Protocols

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For immunoblotting, the following primary antibodies were used: anti‐ULK1 [clone D8H5] (Cell Signaling Technology, Rabbit MAb, Cat# 8054S, 1:1,000, RRID:AB_11178668), anti‐ATG14 [clone D1A1N] (Cell Signaling Technology, Rabbit MAb, Cat# 96752. 1:1,000, RRID:AB_2737056), pS29 ATG14 [clone D4B8M] (Cell Signaling Technology, Cat# 92340S, 1:1,000, RRID:AB_2800182); anti‐Vinculin [clone EPR 8185], (Abcam, Rabbit MAb, Cat# ab129002; 1:1,000, RRID:AB_2800182. Secondary antibodies for immunoblotting were purchased from Thermo Fisher Scientific. For immunocytochemistry, the following primary antibodies were used: anti‐ATPB [clone 3D5] (Abcam, Cat# ab14730; Mouse mAb, 1:500; RRID:AB_301438); anti‐BNIP3L/Nix [clone D4R4B] (Cell Signaling Technology, Cat# 12396, Rabbit mAb, 1:100; RRID:AB_2688036); anti‐ULK1 [clone D8H5] (Cell Signaling Technology, Rabbit mAb, Cat# 8054S, 1:500, RRID:AB_11178668); anti‐WIPI2 (Bio‐Rad, Cat# MCA5780GA, Mouse mAb, 1:500; RRID:AB_11178668). Secondary antibodies for immunocytochemistry were purchased from Invitrogen (Life Technologies) and used at 1:500. The DNA constructs used (Flag‐ULK1 [DU45617], mCherry‐GFP‐FIS 101‐152 [DU55502]) were generated at the MRC PPU Reagents and Services and are available to purchase online https://mrcppureagents.dundee.ac.uk.

Long M., Sanchez‐Martinez A., Longo M., Suomi F., Stenlund H., Johansson A.I., Ehsan H., Salo V.T., Montava‐Garriga L., Naddafi S., Ikonen E., Ganley I.G., Whitworth A.J, & McWilliams T.G. (2022). DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron depletion. The EMBO Journal, 41(10), e109390.

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