P47phox

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P47phox is a protein subunit of the NADPH oxidase complex. It plays a critical role in the activation and regulation of the NADPH oxidase enzyme, which is responsible for the production of reactive oxygen species in various cell types.

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P47phox-/- mice (4 citations) P47phox−/− (also called NCF-1−/−) mice (2 citations) P47phox–/–58 (2 citations)

6 protocols using p47phox

Genetic Knockout Mice for Inflammation

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The p47^phox-deficient (p47^phox-/-, #004742), NLRP3^-/- (#017970), Caspase-1^-/- (#016621), IL-18^-/- (#004130), IL-1R1^-/- (#003245) and wild type (WT) mice were on C57BL/6 background and were purchased from the Jackson Laboratory (Bar Harbor, Maine, USA). Mice were maintained in micro-isolator cages, fed with standard laboratory chow diet and water, and housed in the animal colony at the animal center of the Third Military Medical University (TMMU). Mice approximately 12 weeks of age were used for these experiments. All animals received humane care according to the criteria outlined in the "Guide for the Care and Use of Laboratory Animals" prepared by the National Academy of Sciences and published by the National Institutes of Health (NIH publication 86–23 revised 1985).

Guo S., Yang C., Diao B., Huang X., Jin M., Chen L., Yan W., Ning Q., Zheng L., Wu Y, & Chen Y. (2015). The NLRP3 Inflammasome and IL-1β Accelerate Immunologically Mediated Pathology in Experimental Viral Fulminant Hepatitis. PLoS Pathogens, 11(9), e1005155.

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Mice Strain Characterization Protocol

Cited 1 time

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BALB/c mice were purchased from Charles River Laboratory,
p47phox^-/- (39 (link)) and ACTB-ECFP mice (40 (link))
mice from Jackson Laboratory, and C3(1)-Tag mice (21 (link)) from the NIH mouse repository. LysM-EGFP mice
(20 (link)) were provided by Dr. Mark Looney
(UCSF). All procedures were approved by the Cold Spring Harbor Laboratory (CSHL)
Institutional Animal Care and Use Committee and were conducted in accordance
with the NIH Guide for the Care and Use of Laboratory Animals.

Park J., Wysocki R.W., Amoozgar Z., Maiorino L., Fein M.R., Jorns J., Schott A.F., Kinugasa-Katayama Y., Lee Y., Won N.H., Nakasone E.S., Hearn S.A., Küttner V., Qiu J., Almeida A.S., Perurena N., Kessenbrock K., Goldberg M.S, & Egeblad M. (2016). Cancer cells induce metastasis-supporting neutrophil extracellular DNA traps. Science translational medicine, 8(361), 361ra138.

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Murine Genetic Models for Inflammation Research

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Male C57BL/6J, intercellular adhesion molecule-1 (Icam-1)^-/-, and p47^phox-/- mice were purchased from the Jackson Laboratories (Bar Harbor, ME) and housed in a temperature-controlled, 12-hour light/12-hour dark room. Cxcl1^-/- mice on a C57BL/6 background were kind gifts from Dr Sergio Lira (Mount Sinai, NY) and were further back-crossed into a C57BL/6J background for 5 more generations at the National Institute on Alcohol Abuse and Alcoholism animal facility, with C57BL/6J mice being used as wild-type (WT) controls. In some experiments, heterozygous breeding of Cxcl1^+/- mice was performed to obtain Cxcl1^-/- and littermate WT controls. Animal care was in accordance with the guidelines of the National Institutes of Health. The animal experiments were approved by the National Institute on Alcohol Abuse and Alcoholism Animal Care and Use Committee.

Zhou Z., Xu M.J., Cai Y., Wang W., Jiang J.X., Varga Z.V., Feng D., Pacher P., Kunos G., Torok N.J, & Gao B. (2018). Neutrophil–Hepatic Stellate Cell Interactions Promote Fibrosis in Experimental Steatohepatitis. Cellular and Molecular Gastroenterology and Hepatology, 5(3), 399-413.

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Type 2 Diabetes Mouse Vascular Study

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All experiments were performed according to the American Guidelines for the Ethical Care of Animals and were approved by EVMS Animal Care and Use Committee. Type 2 diabetic, male mice (db⁻/db⁻, 8 to 10 week-old) and their homologous controls, as well as knockout mice of the p47phox expression (p47^phox−/−, 8 to 10 weeks-old males) were purchased from Jackson Laboratories (Bar Harbor, ME). All mice were housed in groups of five mice, maintained at a temperature of 23 °C with 12 h light/dark cycles and fed a solid standard diet (Na⁺ content 0.4%) and water. Blood glucose, body weight and SBP were measured and then mice were sacrificed by isoflurane and were not flushed before harvesting the arteries, and then mesenteric resistance arteries (MRA) were harvested immediately and placed in PSS solution (composition in mM: NaCl 118; KCl 4.7; CaCl₂ 2.5; KH₂PO₄ 1.2; MgSO₄×7H₂O 1.2; NaHCO₃ 25 and glucose 11, pH=7.4) and processed appropriately for further studies.

Kassan M., Ait-Aissa K., Ali M., Trebak M, & Matrougui K. (2015). Augmented EGF receptor tyrosine kinase activity impairs vascular function by NADPH oxidase-dependent mechanism in type 2 diabetic mouse. Biochimica et biophysica acta, 1853(10 Pt A), 2404-2410.

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Isolation of Mouse Aortic Endothelial Cells

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Mouse aortic endothelial cells (MAEC) were isolated from the thoracic and abdominal aortas of various control and knockout mouse lines. Wild-type C57Bl/6 and p47^phox knockout mice were purchased from Jackson Laboratories (Bar Harbor, Maine). Caveolin-1 (cav1) knockout mice were kindly provided by Dr. Marek Drab (Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany). eNOS knockout mice were kindly provided by Dr. Mark C. Fishman and Dr. Paul Huang (Cardiovascular Research Center, Harvard Medical School, Charlestown, MA). All animals were maintained according to the approved Institutional Animal Care and Use Committee protocol by Emory University.

Ni C.W., Kumar S., Ankeny C.J, & Jo H. (2014). Development of immortalized mouse aortic endothelial cell lines. Vascular Cell, 6, 7.

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Mouse Model Generation for Immunology

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Wild-type (WT) C57BL/6 and IL-12p35−/− mice were obtained from Jackson Laboratory (24 (link)). Initial breeding pairs of p47phox−/− transgenic mice also from Jackson Laboratory were kindly provided by R. Brandes and subsequently backcrossed to the C57BL/6 background for 10 generations (25 (link)). IL-23p19−/− mice were kindly provided by N. Ghilardi (Genentech) (26 (link)). The animals were bred and fed under pathogen-free conditions in secluded scantainers. All animal experiments were approved by the Institutional Animal Care and Use Committee of Goethe University Medical Faculty and the Animal Protection Agency of the Federal State of Hessen.

Richter C., Herrero San Juan M., Weigmann B., Bergis D., Dauber K., Muders M.H., Baretton G.B., Pfeilschifter J.M., Bonig H., Brenner S, & Radeke H.H. (2017). Defective IL-23/IL-17 Axis Protects p47phox−/− Mice from Colon Cancer. Frontiers in Immunology, 8, 44.

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