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Distilled water

Manufactured by Tedia
Sourced in United States

Distilled water (DW) is a highly purified form of water that has been subjected to a distillation process to remove impurities. It is a clear, odorless, and tasteless liquid that meets strict purity standards. Distilled water is commonly used as a solvent, diluent, or reagent in various laboratory applications and industrial processes where high-purity water is required.

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7 protocols using distilled water

1

Polymeric Nanoformulations for Drug Delivery

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Soluplus® (Polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer (PCL-PVAc-PEG)), was kindly donated by BASF (Ludwigshafen, Rhineland-Palatinate, Germany). Methoxy poly(ethylene glycol)-b-poly(d,l-lactide) (mPEG [4000]-b-PLA [2200]) was purchased from Advanced Polymer Materials, Inc. (Montreal, QC, Canada). Poly(ethylene oxide-b-ε-caprolactone) (PEO[5000]-b-PCL[10000]) was purchased from Polymer Source, Inc. (Montreal, QC, Canada). Pluronic® F127 was purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Dulbecco’s phosphate-buffered saline (DPBS), and trypsin were purchased from Corning Inc. (Corning, NY, USA). EtOH and acetonitrile (ACN) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Distilled water (DW) was purchased from Tedia (Fairfield, OH, USA). FEN, Thiazolyl blue tetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO), Polysorbate 80 (Tween 80®), DMA, and Cremophor EL® were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). All other reagents were of at least analytical or HPCL grade.
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2

Formulation and Characterization of mPEG-b-PLA Micelles

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Methoxy poly(ethylene glycol)-b-poly(d,l-lactide) (mPEG[4k]-b-PLA[2.2k]) was purchased from Advanced Polymer Materials Inc. (Montreal, QC, Canada). Soluplus® was obtained from BASF (Ludwigshafen, Rhineland-Palatinate, Germany). EtOH and acetonitrile (ACN) were purchased from Fisher Scientific Ltd. (Waltham, MA, USA). Distilled water (DW) was purchased from Tedia (Fairfield, OH, USA). Methanol (MeOH) was purchased from Honeywell Burdick and Jackson (Ulsan, Korea). Genistein was purchased from LC Laboratories (Woburn, MA, USA). Pluronics® F127, ethyl acetate (EtOAc), and Cremophor EL® were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). All other reagents were of analytical grade or better.
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3

Rapamycin Cytotoxicity Assay Protocol

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Acetonitrile (ACN) and Hanks’ Balanced Salt Solution (HBSS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Distilled water (DW) was purchased from Tedia (Fairfield, OH, USA). Rapamycin was purchased from LC Laboratories® (Woburn, MA, USA). Thiazolyl blue tetrazolium bromide (MTT), tert-Butyl alcohol, dimethyl sulfoxide (DMSO), and fenbendazole were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). The mPEG-b-PCL (MW~2000:2000 Da) was purchased from PolySciTech® (West Lafayette, IN, USA). All other reagents were of analytical reagent grade.
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4

Formulation and Characterization of Polymer-Based Drug Delivery Systems

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Methoxy poly(ethylene glycol)-b-poly(D,L-lactide) (mPEG [4000]-b-PLA [2200]) was purchased from Advanced Polymer Materials, Inc. (Montreal, QC, Canada). The mPEG-b-PCL (MW 2000:2000 Da) was purchased from PolySciTech® (West Lafayette, IN, USA). Soluplus® [Polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer (PCL-PVAc-PEG)] was obtained from BASF (Ludwigshafen, Rhineland-Palatinate, Germany). RAPA and genistein were purchased from LC Laboratories® (Woburn, MA, USA). FEN, dimethyl sulfoxide (DMSO), thiazolyl blue tetrazolium bromide (MTT), tert-butanol, cremophor EL®, and dimethylacetamide (DMA) were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Distilled water (DW) was purchased from Tedia (Fairfield, OH, USA). Acetonitrile (ACN) and ethanol (EtOH) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Methanol (MeOH) was purchased from Honeywell, Burdick & Jackson (Ulsan, Korea). All other reagents were used as received without additional purification and were at least HPLC grade.
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5

Biogenic Amine and Amino Acid Analysis

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BA standards (tyramine hydrochloride, 2-phenylethylamine hydrochloride, putrescine dihydrochloride, cadaverine dihydrochloride, histamine dihydrochloride, tryptamine hydrochloride, spermidine trihydrochloride) and amino acid standards (L-Asp, L-Glu, L-Ser, L-Gly, L-His, L-Thr, L-Arg, γ-aminobutyric acid [GABA], L-Ala, L-Pro, L-Tyr, L-Val, L-Met, L-Iso, L-Leu, L-Phe, L-Trp, and L-Lys) were all purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Dansyl chloride, perchloric acid, sodium hydrogen carbonate, potassium dichromate, and silver nitrate were purchased from Daejung Chemical Co. (Siheung, Korea). Distilled water, acetone, and acetonitrile (HPLC grade) were purchased from Tedia Co. (Fairfield, OH, USA).
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6

Formulation and Characterization of Polymeric Drug Delivery Systems

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Soluplus® (polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer; PCL-PVAc-PEG) was donated by BASF (Ludwigshafen, Rhineland-Palatinate, Germany). Methoxy poly (ethylene glycol)-b-poly (D, L-lactide) (mPEG[4000]-b-PLA[2200]) was purchased from Advanced Polymer Materials Inc. (Montreal, QC, Canada). Poly (ethylene oxide-b-ε-caprolactone) (PEO[2000]-b-PCL was from Polyscitech® (West Lafayette, IN, USA), and Pluronic® F-127 was purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Buffered saline (DPBS), trypsin, Cremophor EL®, Matrigel, D–luciferin potassium salt (GoldBio, St. Louis, MO, USA), and Roswell Park Memorial Institute medium (RPMI 1640) were purchased from Corning Inc. (New York, NY, USA). PTX, SRF, and genistein were purchased from LC Laboratories (Woburn, MA, USA). Methanol was purchased from Honeywell Burdick & Jackson (Ulsan, Republic of Korea), and ethanol and acetonitrile (ACN) were purchased from Fisher Scientific Ltd (Waltham, MA, USA). Distilled water was purchased from Tedia (Fairfield, OH, USA) and Tert-Butanol was from Sigma-Aldrich Corp. (St. Louis, MO, USA). All solvents for high-performance liquid chromatography (HPLC) were HPLC grade or higher.
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7

Amino Acid Derivatization for Analysis

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Dansyl chloride, TRY hydrochloride, PHE hydrochloride, PUT dihydrochloride, CAD dihydrochloride, HIS dihydrochloride, TYR hydrochloride, SPD trihydrochloride, and SPM tetrahydrochloride were all purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Perchloric acid, sodium hydrogen carbonate, l-proline, potassium dichromate, and silver nitrate were purchased from Daejung Chemical Co. (Siheung, Korea). Distilled water, acetone, and acetonitrile (HPLC grade) were purchased from Tedia Co. (Fairfield, OH, USA).
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